Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody

The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CP...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jiajia Sun, Liqiang Liu, Shanshan Song, Gang Cui, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	capsaicinoids immunochromatographic strip monoclonal antibody waste oil Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fdf573e8518646c0876a151ba63917fd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fdf573e8518646c0876a151ba63917fd
record_format	dspace
spelling	oai:doaj.org-article:fdf573e8518646c0876a151ba63917fd2021-11-26T11:19:46ZDevelopment of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody0954-01051465-344310.1080/09540105.2018.1490394https://doaj.org/article/fdf573e8518646c0876a151ba63917fd2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1490394https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.Jiajia SunLiqiang LiuShanshan SongGang CuiHua KuangTaylor & Francis Grouparticlecapsaicinoidsimmunochromatographic stripmonoclonal antibodywaste oilAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 930-940 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	capsaicinoids immunochromatographic strip monoclonal antibody waste oil Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	capsaicinoids immunochromatographic strip monoclonal antibody waste oil Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Jiajia Sun Liqiang Liu Shanshan Song Gang Cui Hua Kuang Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
description	The problem of waste oil is a food safety issue that requires urgent resolution. In this study, a novel hapten was designed for the rapid detection of capsaicinoids, a marker of waste oil, and a monoclonal antibody (MAb) 5D7 was generated that is specific for three major capsaicinoids: capsaicin (CPC), dihydrocapsaicin (DCPC), and N-vanillylnonanamide (N-V). The half maximal inhibitory concentrations of MAb 5D7 for CPC, DCPC, and N-V were 1.12, 0.8, and 0.87 ng/mL, respectively. An immunochromatographic strip test based on MAb 5D7 was established for these three capsaicinoids, with visual inspection limits of <1.0 ng/mL, which satisfies the requirement for the rapid on-site inspection of waste oil.
format	article
author	Jiajia Sun Liqiang Liu Shanshan Song Gang Cui Hua Kuang
author_facet	Jiajia Sun Liqiang Liu Shanshan Song Gang Cui Hua Kuang
author_sort	Jiajia Sun
title	Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
title_short	Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
title_full	Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
title_fullStr	Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
title_full_unstemmed	Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
title_sort	development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/fdf573e8518646c0876a151ba63917fd
work_keys_str_mv	AT jiajiasun developmentofanimmunochromatographicstripassayforthreemajorcapsaicinoidsbasedonanultrasensitivemonoclonalantibody AT liqiangliu developmentofanimmunochromatographicstripassayforthreemajorcapsaicinoidsbasedonanultrasensitivemonoclonalantibody AT shanshansong developmentofanimmunochromatographicstripassayforthreemajorcapsaicinoidsbasedonanultrasensitivemonoclonalantibody AT gangcui developmentofanimmunochromatographicstripassayforthreemajorcapsaicinoidsbasedonanultrasensitivemonoclonalantibody AT huakuang developmentofanimmunochromatographicstripassayforthreemajorcapsaicinoidsbasedonanultrasensitivemonoclonalantibody
_version_	1718409574001672192

Development of an immunochromatographic strip assay for three major capsaicinoids based on an ultrasensitive monoclonal antibody

Ejemplares similares