GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic

Abstract GABA counteracts wide range of stresses through regulation of GABA shunt pathway in plants. Although, GABA assisted tolerance against As toxicity in plants is still unexplored. We have examined GABA induced tolerance in rice seedlings with two exposure periods of GABA i.e., short term and l...

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Autores principales:	Navin Kumar, Arvind Kumar Dubey, Atul Kumar Upadhyay, Ambedkar Gautam, Ruma Ranjan, Saripella Srikishna, Nayan Sahu, Soumit Kumar Behera, Shekhar Mallick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdf7eef6c526494783dfcdbd4b1d0009
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spelling	oai:doaj.org-article:fdf7eef6c526494783dfcdbd4b1d00092021-12-02T15:04:51ZGABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic10.1038/s41598-017-09428-22045-2322https://doaj.org/article/fdf7eef6c526494783dfcdbd4b1d00092017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09428-2https://doaj.org/toc/2045-2322Abstract GABA counteracts wide range of stresses through regulation of GABA shunt pathway in plants. Although, GABA assisted tolerance against As toxicity in plants is still unexplored. We have examined GABA induced tolerance in rice seedlings with two exposure periods of GABA i.e., short term and long term. Results showed that accumulation of GABA reduced the expressions of Lsi-1 and Lsi-2 transporter genes, which ultimately decreased the accumulation of As in rice seedlings. The accumulation of GABA also modulated the gene expression of GABA shunt pathway and activity of antioxidant enzymes, which strongly induced the tolerance in plants. Antioxidant enzymes such as CAT, POD, GPX and SOD showed maximum alteration in activity with GABA accretion. In both exposure periods, long term accumulation of GABA was highly efficient to provide tolerance to plants against As(III), while higher level of GABA at short term was toxic. Tolerance responses of GABA towards As(III) was reflected by minimal changes in various physiological (WUE, A, gs, PhiPS2, qp, NPQ, ETR and Trmmol) and growth parameters with concomitant accumulation. Oxidative stress marker such as TBARS and H2O2 contents were reduced with GABA accumulation. These results suggested that GABA sturdily inhibits As accumulation and provides tolerance towards As(III).Navin KumarArvind Kumar DubeyAtul Kumar UpadhyayAmbedkar GautamRuma RanjanSaripella SrikishnaNayan SahuSoumit Kumar BeheraShekhar MallickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Navin Kumar Arvind Kumar Dubey Atul Kumar Upadhyay Ambedkar Gautam Ruma Ranjan Saripella Srikishna Nayan Sahu Soumit Kumar Behera Shekhar Mallick GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
description	Abstract GABA counteracts wide range of stresses through regulation of GABA shunt pathway in plants. Although, GABA assisted tolerance against As toxicity in plants is still unexplored. We have examined GABA induced tolerance in rice seedlings with two exposure periods of GABA i.e., short term and long term. Results showed that accumulation of GABA reduced the expressions of Lsi-1 and Lsi-2 transporter genes, which ultimately decreased the accumulation of As in rice seedlings. The accumulation of GABA also modulated the gene expression of GABA shunt pathway and activity of antioxidant enzymes, which strongly induced the tolerance in plants. Antioxidant enzymes such as CAT, POD, GPX and SOD showed maximum alteration in activity with GABA accretion. In both exposure periods, long term accumulation of GABA was highly efficient to provide tolerance to plants against As(III), while higher level of GABA at short term was toxic. Tolerance responses of GABA towards As(III) was reflected by minimal changes in various physiological (WUE, A, gs, PhiPS2, qp, NPQ, ETR and Trmmol) and growth parameters with concomitant accumulation. Oxidative stress marker such as TBARS and H2O2 contents were reduced with GABA accumulation. These results suggested that GABA sturdily inhibits As accumulation and provides tolerance towards As(III).
format	article
author	Navin Kumar Arvind Kumar Dubey Atul Kumar Upadhyay Ambedkar Gautam Ruma Ranjan Saripella Srikishna Nayan Sahu Soumit Kumar Behera Shekhar Mallick
author_facet	Navin Kumar Arvind Kumar Dubey Atul Kumar Upadhyay Ambedkar Gautam Ruma Ranjan Saripella Srikishna Nayan Sahu Soumit Kumar Behera Shekhar Mallick
author_sort	Navin Kumar
title	GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
title_short	GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
title_full	GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
title_fullStr	GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
title_full_unstemmed	GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic
title_sort	gaba accretion reduces lsi-1 and lsi-2 gene expressions and modulates physiological responses in oryza sativa to provide tolerance towards arsenic
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fdf7eef6c526494783dfcdbd4b1d0009
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GABA accretion reduces Lsi-1 and Lsi-2 gene expressions and modulates physiological responses in Oryza sativa to provide tolerance towards arsenic

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