Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.

ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medi...

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Autores principales:	Yan Hui Yang, Chao Jie Wang, Rui Fang Li, Yan Jie Yi, Lei Zeng, Heng Yang, Chang Fu Zhang, Kai Yi Song, Si Jiao Guo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdf9418df1d54fa8b37a1a56e30a40fd
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spelling	oai:doaj.org-article:fdf9418df1d54fa8b37a1a56e30a40fd2021-12-02T20:10:02ZTranscriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.1932-620310.1371/journal.pone.0253188https://doaj.org/article/fdf9418df1d54fa8b37a1a56e30a40fd2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253188https://doaj.org/toc/1932-6203ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medicinal plant. Here, we scanned R. glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R. glutinosa.Yan Hui YangChao Jie WangRui Fang LiYan Jie YiLei ZengHeng YangChang Fu ZhangKai Yi SongSi Jiao GuoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253188 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Hui Yang Chao Jie Wang Rui Fang Li Yan Jie Yi Lei Zeng Heng Yang Chang Fu Zhang Kai Yi Song Si Jiao Guo Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
description	ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medicinal plant. Here, we scanned R. glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R. glutinosa.
format	article
author	Yan Hui Yang Chao Jie Wang Rui Fang Li Yan Jie Yi Lei Zeng Heng Yang Chang Fu Zhang Kai Yi Song Si Jiao Guo
author_facet	Yan Hui Yang Chao Jie Wang Rui Fang Li Yan Jie Yi Lei Zeng Heng Yang Chang Fu Zhang Kai Yi Song Si Jiao Guo
author_sort	Yan Hui Yang
title	Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
title_short	Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
title_full	Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
title_fullStr	Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
title_full_unstemmed	Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.
title_sort	transcriptome-based identification and expression characterization of rgabcc transporters in rehmannia glutinosa.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fdf9418df1d54fa8b37a1a56e30a40fd
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Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa.

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