Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.

<h4>Background</h4>Campylobacter jejuni is a common cause of acute gastroenteritis and is also associated with the post-infectious neuropathies, Guillain-Barré and Miller Fisher syndromes. In the Cape Town area of South Africa, C. jejuni strains with Penner heat-stable (HS) serotype HS:4...

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Autores principales:	Beatriz Quiñones, Magalie R Guilhabert, William G Miller, Robert E Mandrell, Albert J Lastovica, Craig T Parker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fdfa888a70ff49e5976f0932e8a89bf6
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spelling	oai:doaj.org-article:fdfa888a70ff49e5976f0932e8a89bf62021-11-25T06:12:43ZComparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.1932-620310.1371/journal.pone.0002015https://doaj.org/article/fdfa888a70ff49e5976f0932e8a89bf62008-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18431496/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Campylobacter jejuni is a common cause of acute gastroenteritis and is also associated with the post-infectious neuropathies, Guillain-Barré and Miller Fisher syndromes. In the Cape Town area of South Africa, C. jejuni strains with Penner heat-stable (HS) serotype HS:41 have been observed to be overrepresented among cases of Guillain-Barré syndrome. The present study examined the genetic content of a collection of 32 South African C. jejuni strains with different serotypes, including 13 HS:41 strains, that were recovered from patients with enteritis, Guillain-Barré or Miller Fisher syndromes. The sequence-based typing methods, multilocus sequence typing and DNA microarrays, were employed to potentially identify distinguishing features within the genomes of these C. jejuni strains with various disease outcomes.<h4>Methodology/principal findings</h4>Comparative genomic analyses demonstrated that the HS:41 South African strains were clearly distinct from the other South African strains. Further DNA microarray analysis demonstrated that the HS:41 strains from South African patients with the Guillain-Barré syndrome or enteritis were highly similar in gene content. Interestingly, the South African HS:41 strains were distinct in gene content when compared to HS:41 strains from other geographical locations due to the presence of genomic islands, referred to as Campylobacter jejuni integrated elements (CJIEs). Only the integrated element CJIE1, a Campylobacter Mu-like prophage, was present in the South African HS:41 strains whereas this element was absent in two closely-related HS:41 strains from Mexico. A more distantly-related HS:41 strain from Canada possessed both integrated elements CJIE1 and CJIE2.<h4>Conclusion/significance</h4>These findings demonstrate that CJIEs may contribute to the differentiation of closely-related C. jejuni strains. In addition, the presence of bacteriophage-related genes in CJIE1 may contribute to the genomic diversity of C. jejuni strains. This comparative genomic analysis of C. jejuni provides fundamental information that potentially could lead to improved methods for analyzing the epidemiology of disease outbreaks.Beatriz QuiñonesMagalie R GuilhabertWilliam G MillerRobert E MandrellAlbert J LastovicaCraig T ParkerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 4, p e2015 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Beatriz Quiñones Magalie R Guilhabert William G Miller Robert E Mandrell Albert J Lastovica Craig T Parker Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
description	<h4>Background</h4>Campylobacter jejuni is a common cause of acute gastroenteritis and is also associated with the post-infectious neuropathies, Guillain-Barré and Miller Fisher syndromes. In the Cape Town area of South Africa, C. jejuni strains with Penner heat-stable (HS) serotype HS:41 have been observed to be overrepresented among cases of Guillain-Barré syndrome. The present study examined the genetic content of a collection of 32 South African C. jejuni strains with different serotypes, including 13 HS:41 strains, that were recovered from patients with enteritis, Guillain-Barré or Miller Fisher syndromes. The sequence-based typing methods, multilocus sequence typing and DNA microarrays, were employed to potentially identify distinguishing features within the genomes of these C. jejuni strains with various disease outcomes.<h4>Methodology/principal findings</h4>Comparative genomic analyses demonstrated that the HS:41 South African strains were clearly distinct from the other South African strains. Further DNA microarray analysis demonstrated that the HS:41 strains from South African patients with the Guillain-Barré syndrome or enteritis were highly similar in gene content. Interestingly, the South African HS:41 strains were distinct in gene content when compared to HS:41 strains from other geographical locations due to the presence of genomic islands, referred to as Campylobacter jejuni integrated elements (CJIEs). Only the integrated element CJIE1, a Campylobacter Mu-like prophage, was present in the South African HS:41 strains whereas this element was absent in two closely-related HS:41 strains from Mexico. A more distantly-related HS:41 strain from Canada possessed both integrated elements CJIE1 and CJIE2.<h4>Conclusion/significance</h4>These findings demonstrate that CJIEs may contribute to the differentiation of closely-related C. jejuni strains. In addition, the presence of bacteriophage-related genes in CJIE1 may contribute to the genomic diversity of C. jejuni strains. This comparative genomic analysis of C. jejuni provides fundamental information that potentially could lead to improved methods for analyzing the epidemiology of disease outbreaks.
format	article
author	Beatriz Quiñones Magalie R Guilhabert William G Miller Robert E Mandrell Albert J Lastovica Craig T Parker
author_facet	Beatriz Quiñones Magalie R Guilhabert William G Miller Robert E Mandrell Albert J Lastovica Craig T Parker
author_sort	Beatriz Quiñones
title	Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
title_short	Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
title_full	Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
title_fullStr	Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
title_full_unstemmed	Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.
title_sort	comparative genomic analysis of clinical strains of campylobacter jejuni from south africa.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/fdfa888a70ff49e5976f0932e8a89bf6
work_keys_str_mv	AT beatrizquinones comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica AT magalierguilhabert comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica AT williamgmiller comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica AT robertemandrell comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica AT albertjlastovica comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica AT craigtparker comparativegenomicanalysisofclinicalstrainsofcampylobacterjejunifromsouthafrica
_version_	1718414082055340032

Comparative genomic analysis of clinical strains of Campylobacter jejuni from South Africa.

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