Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—

Detection methods for small biological molecules are needed to facilitate analysis of physiological and pathological mechanisms. We aimed to construct a 2-mercaptobenzimidazole modified gold nanoparticle electrode for detection of uremia-related substances, e.g. uric acid (UA) and indoxyl sulfate (I...

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Autores principales:	Kyoko FUJITA, Misaki SEKIDO, Kohei KANNO, Kio HATAE, Kimiyoshi ICHIDA
Formato:	article
Lenguaje:	EN JA
Publicado:	The Electrochemical Society of Japan 2021
Materias:	modified gold nanoparticle electrode indoxyl sulfate biomolecular detection transport protein Technology T Physical and theoretical chemistry QD450-801
Acceso en línea:	https://doaj.org/article/fdfaa5868b1e49fa8812ae52d809b125
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spelling	oai:doaj.org-article:fdfaa5868b1e49fa8812ae52d809b1252021-11-05T00:17:27ZDevelopment of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—2186-245110.5796/electrochemistry.21-00072https://doaj.org/article/fdfaa5868b1e49fa8812ae52d809b1252021-11-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/electrochemistry/89/6/89_21-00072/_html/-char/enhttps://doaj.org/toc/2186-2451Detection methods for small biological molecules are needed to facilitate analysis of physiological and pathological mechanisms. We aimed to construct a 2-mercaptobenzimidazole modified gold nanoparticle electrode for detection of uremia-related substances, e.g. uric acid (UA) and indoxyl sulfate (IS), excreted via transporters expressed on cultured cells. This electrode detected the current changes in phosphate buffer at different potentials as the concentrations of ascorbic acid, UA, dopamine, and IS were linearly increased in 1 µg/mL increments. Real-time detection of IS excretion via ATP-binding cassette transporter G2 (ABCG2) expression was performed in a transcellular transport model with amperometric measurement. The electrode was highly sensitive to the current changes with IS even in a serum-free culture medium. We observed an increase in current of approximately 0.10 µA per mm2 of polycrystalline electrode surface area with each 1 µg/mL increase in IS concentration. The current increased with time when the electrode was exposed to cells transfected with ABCG2 plasmid in tissue culture insert, indicating that IS excretion occurred via the transporter encoded by ABCG2. In conclusion, the electrode could be successfully used for the real-time detection of IS excreted via ABCG2 expressed on cultured cells.Kyoko FUJITAMisaki SEKIDOKohei KANNOKio HATAEKimiyoshi ICHIDAThe Electrochemical Society of Japanarticlemodified gold nanoparticle electrodeindoxyl sulfatebiomolecular detectiontransport proteinTechnologyTPhysical and theoretical chemistryQD450-801ENJAElectrochemistry, Vol 89, Iss 6, Pp 552-556 (2021)
institution	DOAJ
collection	DOAJ
language	EN JA
topic	modified gold nanoparticle electrode indoxyl sulfate biomolecular detection transport protein Technology T Physical and theoretical chemistry QD450-801
spellingShingle	modified gold nanoparticle electrode indoxyl sulfate biomolecular detection transport protein Technology T Physical and theoretical chemistry QD450-801 Kyoko FUJITA Misaki SEKIDO Kohei KANNO Kio HATAE Kimiyoshi ICHIDA Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
description	Detection methods for small biological molecules are needed to facilitate analysis of physiological and pathological mechanisms. We aimed to construct a 2-mercaptobenzimidazole modified gold nanoparticle electrode for detection of uremia-related substances, e.g. uric acid (UA) and indoxyl sulfate (IS), excreted via transporters expressed on cultured cells. This electrode detected the current changes in phosphate buffer at different potentials as the concentrations of ascorbic acid, UA, dopamine, and IS were linearly increased in 1 µg/mL increments. Real-time detection of IS excretion via ATP-binding cassette transporter G2 (ABCG2) expression was performed in a transcellular transport model with amperometric measurement. The electrode was highly sensitive to the current changes with IS even in a serum-free culture medium. We observed an increase in current of approximately 0.10 µA per mm2 of polycrystalline electrode surface area with each 1 µg/mL increase in IS concentration. The current increased with time when the electrode was exposed to cells transfected with ABCG2 plasmid in tissue culture insert, indicating that IS excretion occurred via the transporter encoded by ABCG2. In conclusion, the electrode could be successfully used for the real-time detection of IS excreted via ABCG2 expressed on cultured cells.
format	article
author	Kyoko FUJITA Misaki SEKIDO Kohei KANNO Kio HATAE Kimiyoshi ICHIDA
author_facet	Kyoko FUJITA Misaki SEKIDO Kohei KANNO Kio HATAE Kimiyoshi ICHIDA
author_sort	Kyoko FUJITA
title	Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
title_short	Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
title_full	Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
title_fullStr	Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
title_full_unstemmed	Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—
title_sort	development of a molecular recognition electrode and investigation of a biomolecular application in non-aqueous media —electrochemical detection of uremia-related substances excreted via atp-binding cassette transporter g2—
publisher	The Electrochemical Society of Japan
publishDate	2021
url	https://doaj.org/article/fdfaa5868b1e49fa8812ae52d809b125
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Development of a Molecular Recognition Electrode and Investigation of a Biomolecular Application in Non-Aqueous Media —Electrochemical Detection of Uremia-Related Substances Excreted via ATP-Binding Cassette Transporter G2—

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