Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe

Local interneurons (LNs) mediate complex interactions within the antennal lobe, the primary olfactory system of insects, and the functional analog of the vertebrate olfactory bulb. In the cockroach Periplaneta americana, as in other insects, several types of LNs with distinctive physiological and mo...

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Autores principales:	Debora Fusca, Peter Kloppenburg
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	antennal lobe calcium imaging local interneurons olfaction Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fdff8d6475f84ac3a8c65382d5eef31e
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spelling	oai:doaj.org-article:fdff8d6475f84ac3a8c65382d5eef31e2021-11-15T07:00:26ZTask-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe10.7554/eLife.652172050-084Xe65217https://doaj.org/article/fdff8d6475f84ac3a8c65382d5eef31e2021-09-01T00:00:00Zhttps://elifesciences.org/articles/65217https://doaj.org/toc/2050-084XLocal interneurons (LNs) mediate complex interactions within the antennal lobe, the primary olfactory system of insects, and the functional analog of the vertebrate olfactory bulb. In the cockroach Periplaneta americana, as in other insects, several types of LNs with distinctive physiological and morphological properties can be defined. Here, we combined whole-cell patch-clamp recordings and Ca2+ imaging of individual LNs to analyze the role of spiking and nonspiking LNs in inter- and intraglomerular signaling during olfactory information processing. Spiking GABAergic LNs reacted to odorant stimulation with a uniform rise in [Ca2+]i in the ramifications of all innervated glomeruli. In contrast, in nonspiking LNs, glomerular Ca2+ signals were odorant specific and varied between glomeruli, resulting in distinct, glomerulus-specific tuning curves. The cell type-specific differences in Ca2+ dynamics support the idea that spiking LNs play a primary role in interglomerular signaling, while they assign nonspiking LNs an essential role in intraglomerular signaling.Debora FuscaPeter KloppenburgeLife Sciences Publications Ltdarticleantennal lobecalcium imaginglocal interneuronsolfactionMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antennal lobe calcium imaging local interneurons olfaction Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	antennal lobe calcium imaging local interneurons olfaction Medicine R Science Q Biology (General) QH301-705.5 Debora Fusca Peter Kloppenburg Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
description	Local interneurons (LNs) mediate complex interactions within the antennal lobe, the primary olfactory system of insects, and the functional analog of the vertebrate olfactory bulb. In the cockroach Periplaneta americana, as in other insects, several types of LNs with distinctive physiological and morphological properties can be defined. Here, we combined whole-cell patch-clamp recordings and Ca2+ imaging of individual LNs to analyze the role of spiking and nonspiking LNs in inter- and intraglomerular signaling during olfactory information processing. Spiking GABAergic LNs reacted to odorant stimulation with a uniform rise in [Ca2+]i in the ramifications of all innervated glomeruli. In contrast, in nonspiking LNs, glomerular Ca2+ signals were odorant specific and varied between glomeruli, resulting in distinct, glomerulus-specific tuning curves. The cell type-specific differences in Ca2+ dynamics support the idea that spiking LNs play a primary role in interglomerular signaling, while they assign nonspiking LNs an essential role in intraglomerular signaling.
format	article
author	Debora Fusca Peter Kloppenburg
author_facet	Debora Fusca Peter Kloppenburg
author_sort	Debora Fusca
title	Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
title_short	Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
title_full	Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
title_fullStr	Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
title_full_unstemmed	Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
title_sort	task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/fdff8d6475f84ac3a8c65382d5eef31e
work_keys_str_mv	AT deborafusca taskspecificrolesoflocalinterneuronsforinterandintraglomerularsignalingintheinsectantennallobe AT peterkloppenburg taskspecificrolesoflocalinterneuronsforinterandintraglomerularsignalingintheinsectantennallobe
_version_	1718428533200519168

Task-specific roles of local interneurons for inter- and intraglomerular signaling in the insect antennal lobe

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