Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk

The current study was performed to develop a simple, safe, and cost-effective technique for the biosynthesis of selenium nanoparticles (SeNPs) from lactic acid bacteria (LAB) isolated from human breast milk with antifungal activity against animal pathogenic fungi. The LAB was selected based on their...

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Autores principales:	Mohamed T. El-Saadony, Ahmed M. Saad, Taha F. Taha, Azhar A. Najjar, Nidal M. Zabermawi, Maha M. Nader, Synan F. AbuQamar, Khaled A. El-Tarabily, Ali Salama
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Animals' pathogenic fungi Antifungal metabolites Biosynthesis Lactobacillus paracasei Selenium nanoparticles Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fe02f99a5cc34a709bf700396cc2d415
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spelling	oai:doaj.org-article:fe02f99a5cc34a709bf700396cc2d4152021-11-20T04:56:28ZSelenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk1319-562X10.1016/j.sjbs.2021.07.059https://doaj.org/article/fe02f99a5cc34a709bf700396cc2d4152021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006422https://doaj.org/toc/1319-562XThe current study was performed to develop a simple, safe, and cost-effective technique for the biosynthesis of selenium nanoparticles (SeNPs) from lactic acid bacteria (LAB) isolated from human breast milk with antifungal activity against animal pathogenic fungi. The LAB was selected based on their speed of transforming sodium selenite (Na2SeO3) to SeNPs. Out of the four identified LAB isolates, only one strain produced dark red color within 32 h of incubation, indicating that this isolate was the fastest in transforming Na2SeO3 to SeNPs; and was chosen for the biosynthesis of LAB-SeNPs. The superior isolate was further identified as Lactobacillus paracasei HM1 (MW390875) based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and phylogenetic tree analysis of 16S rRNA sequence alignments. The optimum experimental conditions for the biosynthesis of SeNPs by L. paracasei HM1 were found to be pH (6.0), temperature (35˚C), Na2SeO3 (4.0 mM), reaction time (32 h), and agitation speed (160 rpm). The ultraviolet absorbance of L. paracasei-SeNPs was detected at 300 nm, and the transmission electron microscopy (TEM) captured a diameter range between 3.0 and 50.0 nm. The energy-dispersive X-ray spectroscopy (EDX) and the Fourier-transform infrared spectroscopy (FTIR) provided a clear image of the active groups associated with the stability of L. paracasei-SeNPs. The size of L. paracasei-SeNPs using dynamic light scattering technique was 56.91 ± 1.8 nm, and zeta potential value was −20.1 ± 0.6 mV in one peak. The data also revealed that L. paracasei-SeNPs effectively inhibited the growth of Candida and Fusarium species, and this was further confirmed by scanning electron microscopy (SEM). The current study concluded that the SeNPs obtained from L. paracasei HM1 could be used to prepare biological antifungal formulations effective against major animal pathogenic fungi. The antifungal activity of the biologically synthesized SeNPs using L. paracasei HM1 outperforms the chemically produced SeNPs. In vivo studies showing the antagonistic effect of SeNPs on pathogenic fungi are underway to demonstrate the potential of a therapeutic agent to treat animals against major infectious fungal diseases.Mohamed T. El-SaadonyAhmed M. SaadTaha F. TahaAzhar A. NajjarNidal M. ZabermawiMaha M. NaderSynan F. AbuQamarKhaled A. El-TarabilyAli SalamaElsevierarticleAnimals' pathogenic fungiAntifungal metabolitesBiosynthesisLactobacillus paracaseiSelenium nanoparticlesBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6782-6794 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Animals' pathogenic fungi Antifungal metabolites Biosynthesis Lactobacillus paracasei Selenium nanoparticles Biology (General) QH301-705.5
spellingShingle	Animals' pathogenic fungi Antifungal metabolites Biosynthesis Lactobacillus paracasei Selenium nanoparticles Biology (General) QH301-705.5 Mohamed T. El-Saadony Ahmed M. Saad Taha F. Taha Azhar A. Najjar Nidal M. Zabermawi Maha M. Nader Synan F. AbuQamar Khaled A. El-Tarabily Ali Salama Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
description	The current study was performed to develop a simple, safe, and cost-effective technique for the biosynthesis of selenium nanoparticles (SeNPs) from lactic acid bacteria (LAB) isolated from human breast milk with antifungal activity against animal pathogenic fungi. The LAB was selected based on their speed of transforming sodium selenite (Na2SeO3) to SeNPs. Out of the four identified LAB isolates, only one strain produced dark red color within 32 h of incubation, indicating that this isolate was the fastest in transforming Na2SeO3 to SeNPs; and was chosen for the biosynthesis of LAB-SeNPs. The superior isolate was further identified as Lactobacillus paracasei HM1 (MW390875) based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and phylogenetic tree analysis of 16S rRNA sequence alignments. The optimum experimental conditions for the biosynthesis of SeNPs by L. paracasei HM1 were found to be pH (6.0), temperature (35˚C), Na2SeO3 (4.0 mM), reaction time (32 h), and agitation speed (160 rpm). The ultraviolet absorbance of L. paracasei-SeNPs was detected at 300 nm, and the transmission electron microscopy (TEM) captured a diameter range between 3.0 and 50.0 nm. The energy-dispersive X-ray spectroscopy (EDX) and the Fourier-transform infrared spectroscopy (FTIR) provided a clear image of the active groups associated with the stability of L. paracasei-SeNPs. The size of L. paracasei-SeNPs using dynamic light scattering technique was 56.91 ± 1.8 nm, and zeta potential value was −20.1 ± 0.6 mV in one peak. The data also revealed that L. paracasei-SeNPs effectively inhibited the growth of Candida and Fusarium species, and this was further confirmed by scanning electron microscopy (SEM). The current study concluded that the SeNPs obtained from L. paracasei HM1 could be used to prepare biological antifungal formulations effective against major animal pathogenic fungi. The antifungal activity of the biologically synthesized SeNPs using L. paracasei HM1 outperforms the chemically produced SeNPs. In vivo studies showing the antagonistic effect of SeNPs on pathogenic fungi are underway to demonstrate the potential of a therapeutic agent to treat animals against major infectious fungal diseases.
format	article
author	Mohamed T. El-Saadony Ahmed M. Saad Taha F. Taha Azhar A. Najjar Nidal M. Zabermawi Maha M. Nader Synan F. AbuQamar Khaled A. El-Tarabily Ali Salama
author_facet	Mohamed T. El-Saadony Ahmed M. Saad Taha F. Taha Azhar A. Najjar Nidal M. Zabermawi Maha M. Nader Synan F. AbuQamar Khaled A. El-Tarabily Ali Salama
author_sort	Mohamed T. El-Saadony
title	Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
title_short	Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
title_full	Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
title_fullStr	Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
title_full_unstemmed	Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
title_sort	selenium nanoparticles from lactobacillus paracasei hm1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/fe02f99a5cc34a709bf700396cc2d415
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Selenium nanoparticles from Lactobacillus paracasei HM1 capable of antagonizing animal pathogenic fungi as a new source from human breast milk

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