Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales

ABSTRACT Hydraulic fracturing of black shale formations has greatly increased United States oil and natural gas recovery. However, the accumulation of biomass in subsurface reservoirs and pipelines is detrimental because of possible well souring, microbially induced corrosion, and pore clogging. Tem...

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Autores principales:	Anne E. Booker, Mikayla A. Borton, Rebecca A. Daly, Susan A. Welch, Carrie D. Nicora, David W. Hoyt, Travis Wilson, Samuel O. Purvine, Richard A. Wolfe, Shikha Sharma, Paula J. Mouser, David R. Cole, Mary S. Lipton, Kelly C. Wrighton, Michael J. Wilkins
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Halanaerobium shale thiosulfate Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fe24008a7b814a13a6576bfc18578b1d
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spelling	oai:doaj.org-article:fe24008a7b814a13a6576bfc18578b1d2021-11-15T15:22:05ZSulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales10.1128/mSphereDirect.00257-172379-5042https://doaj.org/article/fe24008a7b814a13a6576bfc18578b1d2017-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphereDirect.00257-17https://doaj.org/toc/2379-5042ABSTRACT Hydraulic fracturing of black shale formations has greatly increased United States oil and natural gas recovery. However, the accumulation of biomass in subsurface reservoirs and pipelines is detrimental because of possible well souring, microbially induced corrosion, and pore clogging. Temporal sampling of produced fluids from a well in the Utica Shale revealed the dominance of Halanaerobium strains within the in situ microbial community and the potential for these microorganisms to catalyze thiosulfate-dependent sulfidogenesis. From these field data, we investigated biogenic sulfide production catalyzed by a Halanaerobium strain isolated from the produced fluids using proteogenomics and laboratory growth experiments. Analysis of Halanaerobium isolate genomes and reconstructed genomes from metagenomic data sets revealed the conserved presence of rhodanese-like proteins and anaerobic sulfite reductase complexes capable of converting thiosulfate to sulfide. Shotgun proteomics measurements using a Halanaerobium isolate verified that these proteins were more abundant when thiosulfate was present in the growth medium, and culture-based assays identified thiosulfate-dependent sulfide production by the same isolate. Increased production of sulfide and organic acids during the stationary growth phase suggests that fermentative Halanaerobium uses thiosulfate to remove excess reductant. These findings emphasize the potential detrimental effects that could arise from thiosulfate-reducing microorganisms in hydraulically fractured shales, which are undetected by current industry-wide corrosion diagnostics. IMPORTANCE Although thousands of wells in deep shale formations across the United States have been hydraulically fractured for oil and gas recovery, the impact of microbial metabolism within these environments is poorly understood. Our research demonstrates that dominant microbial populations in these subsurface ecosystems contain the conserved capacity for the reduction of thiosulfate to sulfide and that this process is likely occurring in the environment. Sulfide generation (also known as “souring”) is considered deleterious in the oil and gas industry because of both toxicity issues and impacts on corrosion of the subsurface infrastructure. Critically, the capacity for sulfide generation via reduction of sulfate was not detected in our data sets. Given that current industry wellhead tests for sulfidogenesis target canonical sulfate-reducing microorganisms, these data suggest that new approaches to the detection of sulfide-producing microorganisms may be necessary.Anne E. BookerMikayla A. BortonRebecca A. DalySusan A. WelchCarrie D. NicoraDavid W. HoytTravis WilsonSamuel O. PurvineRichard A. WolfeShikha SharmaPaula J. MouserDavid R. ColeMary S. LiptonKelly C. WrightonMichael J. WilkinsAmerican Society for MicrobiologyarticleHalanaerobiumshalethiosulfateMicrobiologyQR1-502ENmSphere, Vol 2, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Halanaerobium shale thiosulfate Microbiology QR1-502
spellingShingle	Halanaerobium shale thiosulfate Microbiology QR1-502 Anne E. Booker Mikayla A. Borton Rebecca A. Daly Susan A. Welch Carrie D. Nicora David W. Hoyt Travis Wilson Samuel O. Purvine Richard A. Wolfe Shikha Sharma Paula J. Mouser David R. Cole Mary S. Lipton Kelly C. Wrighton Michael J. Wilkins Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
description	ABSTRACT Hydraulic fracturing of black shale formations has greatly increased United States oil and natural gas recovery. However, the accumulation of biomass in subsurface reservoirs and pipelines is detrimental because of possible well souring, microbially induced corrosion, and pore clogging. Temporal sampling of produced fluids from a well in the Utica Shale revealed the dominance of Halanaerobium strains within the in situ microbial community and the potential for these microorganisms to catalyze thiosulfate-dependent sulfidogenesis. From these field data, we investigated biogenic sulfide production catalyzed by a Halanaerobium strain isolated from the produced fluids using proteogenomics and laboratory growth experiments. Analysis of Halanaerobium isolate genomes and reconstructed genomes from metagenomic data sets revealed the conserved presence of rhodanese-like proteins and anaerobic sulfite reductase complexes capable of converting thiosulfate to sulfide. Shotgun proteomics measurements using a Halanaerobium isolate verified that these proteins were more abundant when thiosulfate was present in the growth medium, and culture-based assays identified thiosulfate-dependent sulfide production by the same isolate. Increased production of sulfide and organic acids during the stationary growth phase suggests that fermentative Halanaerobium uses thiosulfate to remove excess reductant. These findings emphasize the potential detrimental effects that could arise from thiosulfate-reducing microorganisms in hydraulically fractured shales, which are undetected by current industry-wide corrosion diagnostics. IMPORTANCE Although thousands of wells in deep shale formations across the United States have been hydraulically fractured for oil and gas recovery, the impact of microbial metabolism within these environments is poorly understood. Our research demonstrates that dominant microbial populations in these subsurface ecosystems contain the conserved capacity for the reduction of thiosulfate to sulfide and that this process is likely occurring in the environment. Sulfide generation (also known as “souring”) is considered deleterious in the oil and gas industry because of both toxicity issues and impacts on corrosion of the subsurface infrastructure. Critically, the capacity for sulfide generation via reduction of sulfate was not detected in our data sets. Given that current industry wellhead tests for sulfidogenesis target canonical sulfate-reducing microorganisms, these data suggest that new approaches to the detection of sulfide-producing microorganisms may be necessary.
format	article
author	Anne E. Booker Mikayla A. Borton Rebecca A. Daly Susan A. Welch Carrie D. Nicora David W. Hoyt Travis Wilson Samuel O. Purvine Richard A. Wolfe Shikha Sharma Paula J. Mouser David R. Cole Mary S. Lipton Kelly C. Wrighton Michael J. Wilkins
author_facet	Anne E. Booker Mikayla A. Borton Rebecca A. Daly Susan A. Welch Carrie D. Nicora David W. Hoyt Travis Wilson Samuel O. Purvine Richard A. Wolfe Shikha Sharma Paula J. Mouser David R. Cole Mary S. Lipton Kelly C. Wrighton Michael J. Wilkins
author_sort	Anne E. Booker
title	Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
title_short	Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
title_full	Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
title_fullStr	Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
title_full_unstemmed	Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales
title_sort	sulfide generation by dominant <italic toggle="yes">halanaerobium</italic> microorganisms in hydraulically fractured shales
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/fe24008a7b814a13a6576bfc18578b1d
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Sulfide Generation by Dominant <italic toggle="yes">Halanaerobium</italic> Microorganisms in Hydraulically Fractured Shales

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