Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as <i>Penicillium oxalicum</i> M1816. The genome was...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/fe2416f3f2f0450fa1277a41a4e6e995 |
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| Sumario: | The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as <i>Penicillium oxalicum</i> M1816. The genome was sequenced and assembled into 30.5 Mb containing 8301 predicted protein-coding genes. In total, 553 genes were associated with carbohydrate metabolism. Genomic CAZymes analysis indicated that <i>P. oxalicum</i> M1816, comprising 39 cellulolytic enzymes and 111 hemicellulases (including 5 feruloyl esterase genes), may play a vital role in wheat bran degradation and FA production. The crude enzyme of strain M1816 could release 1.85 ± 0.08 mg·g<sup>−1</sup> FA from de-starched wheat bran (DSWB) at 12 h, which was significantly higher than other commercial enzymes. Meanwhile, when the strain M1816 was cultured in medium supplemented with DSWB, up to 92.89% of the total alkali-extractable FA was released. The process parameters of solid-state fermentation were optimized to enhance enzyme production. The optimized wheat bran <i>Qu</i> of <i>P. oxalicum</i> M1816 was applied to <i>huangjiu</i> fermentation, and the FA content was increased 12.4-fold compared to the control group. These results suggest that <i>P. oxalicum</i> M1816 is a good candidate for the development of fermented foods bio-fortified with FA. |
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