Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production

The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as <i>Penicillium oxalicum</i> M1816. The genome was...

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Autores principales:	Jing Zhang, Shuangping Liu, Hailong Sun, Zhengfei Jiang, Zhilei Zhou, Xiao Han, Yongxiang Zhou, Honggen Sun, Weibiao Zhou, Jian Mao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ferulic acid <i>Penicillium oxalicum</i> wheat bran <i>huangjiu</i> fermentation whole genome Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/fe2416f3f2f0450fa1277a41a4e6e995
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spelling	oai:doaj.org-article:fe2416f3f2f0450fa1277a41a4e6e9952021-11-25T17:32:50ZEnzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production10.3390/foods101125772304-8158https://doaj.org/article/fe2416f3f2f0450fa1277a41a4e6e9952021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2577https://doaj.org/toc/2304-8158The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as <i>Penicillium oxalicum</i> M1816. The genome was sequenced and assembled into 30.5 Mb containing 8301 predicted protein-coding genes. In total, 553 genes were associated with carbohydrate metabolism. Genomic CAZymes analysis indicated that <i>P. oxalicum</i> M1816, comprising 39 cellulolytic enzymes and 111 hemicellulases (including 5 feruloyl esterase genes), may play a vital role in wheat bran degradation and FA production. The crude enzyme of strain M1816 could release 1.85 ± 0.08 mg·g<sup>−1</sup> FA from de-starched wheat bran (DSWB) at 12 h, which was significantly higher than other commercial enzymes. Meanwhile, when the strain M1816 was cultured in medium supplemented with DSWB, up to 92.89% of the total alkali-extractable FA was released. The process parameters of solid-state fermentation were optimized to enhance enzyme production. The optimized wheat bran <i>Qu</i> of <i>P. oxalicum</i> M1816 was applied to <i>huangjiu</i> fermentation, and the FA content was increased 12.4-fold compared to the control group. These results suggest that <i>P. oxalicum</i> M1816 is a good candidate for the development of fermented foods bio-fortified with FA.Jing ZhangShuangping LiuHailong SunZhengfei JiangZhilei ZhouXiao HanYongxiang ZhouHonggen SunWeibiao ZhouJian MaoMDPI AGarticleferulic acid<i>Penicillium oxalicum</i>wheat bran<i>huangjiu</i> fermentationwhole genomeChemical technologyTP1-1185ENFoods, Vol 10, Iss 2577, p 2577 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ferulic acid <i>Penicillium oxalicum</i> wheat bran <i>huangjiu</i> fermentation whole genome Chemical technology TP1-1185
spellingShingle	ferulic acid <i>Penicillium oxalicum</i> wheat bran <i>huangjiu</i> fermentation whole genome Chemical technology TP1-1185 Jing Zhang Shuangping Liu Hailong Sun Zhengfei Jiang Zhilei Zhou Xiao Han Yongxiang Zhou Honggen Sun Weibiao Zhou Jian Mao Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
description	The present study focused on isolating an efficient enzyme production microorganism for ferulic acid (FA) production from wheat bran. A wild-type cellulase-, xylanase-, and feruloyl esterase-producing strain was isolated and identified as <i>Penicillium oxalicum</i> M1816. The genome was sequenced and assembled into 30.5 Mb containing 8301 predicted protein-coding genes. In total, 553 genes were associated with carbohydrate metabolism. Genomic CAZymes analysis indicated that <i>P. oxalicum</i> M1816, comprising 39 cellulolytic enzymes and 111 hemicellulases (including 5 feruloyl esterase genes), may play a vital role in wheat bran degradation and FA production. The crude enzyme of strain M1816 could release 1.85 ± 0.08 mg·g<sup>−1</sup> FA from de-starched wheat bran (DSWB) at 12 h, which was significantly higher than other commercial enzymes. Meanwhile, when the strain M1816 was cultured in medium supplemented with DSWB, up to 92.89% of the total alkali-extractable FA was released. The process parameters of solid-state fermentation were optimized to enhance enzyme production. The optimized wheat bran <i>Qu</i> of <i>P. oxalicum</i> M1816 was applied to <i>huangjiu</i> fermentation, and the FA content was increased 12.4-fold compared to the control group. These results suggest that <i>P. oxalicum</i> M1816 is a good candidate for the development of fermented foods bio-fortified with FA.
format	article
author	Jing Zhang Shuangping Liu Hailong Sun Zhengfei Jiang Zhilei Zhou Xiao Han Yongxiang Zhou Honggen Sun Weibiao Zhou Jian Mao
author_facet	Jing Zhang Shuangping Liu Hailong Sun Zhengfei Jiang Zhilei Zhou Xiao Han Yongxiang Zhou Honggen Sun Weibiao Zhou Jian Mao
author_sort	Jing Zhang
title	Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
title_short	Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
title_full	Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
title_fullStr	Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
title_full_unstemmed	Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production
title_sort	enzyme production potential of <em>penicillium oxalicum</em> m1816 and its application in ferulic acid production
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fe2416f3f2f0450fa1277a41a4e6e995
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Enzyme Production Potential of <em>Penicillium oxalicum</em> M1816 and Its Application in Ferulic Acid Production

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