Saliva-derived microcosm biofilms grown on different oral surfaces in vitro

Abstract The microbial composition of a specific oral niche could be influenced by initial bacterial adherence, nutrient and physiological property of the local surface. To investigate the influence of nutrient and surface properties on microbial composition, saliva-derived biofilms were grown in ag...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaolan Li, Lin Shang, Bernd W. Brandt, Mark J. Buijs, Sanne Roffel, Cor van Loveren, Wim Crielaard, Susan Gibbs, Dong Mei Deng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/fe2857e0011f4bc0a23c327a7387c9bb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe2857e0011f4bc0a23c327a7387c9bb
record_format	dspace
spelling	oai:doaj.org-article:fe2857e0011f4bc0a23c327a7387c9bb2021-12-02T17:19:18ZSaliva-derived microcosm biofilms grown on different oral surfaces in vitro10.1038/s41522-021-00246-z2055-5008https://doaj.org/article/fe2857e0011f4bc0a23c327a7387c9bb2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00246-zhttps://doaj.org/toc/2055-5008Abstract The microbial composition of a specific oral niche could be influenced by initial bacterial adherence, nutrient and physiological property of the local surface. To investigate the influence of nutrient and surface properties on microbial composition, saliva-derived biofilms were grown in agar on three substrata: Reconstructed Human Gingiva (RHG), a hydroxyapatite (HAP) surface, and a titanium (TI) surface. Agar was mixed with either Brain Heart Infusion (BHI) or Thompson (TP) medium. After 1, 3, or 5 days, biofilm viability (by colony forming units) and microbiome profiles (by 16 S rDNA amplicon sequencing) were determined. On RHG, biofilm viability and composition were similar between BHI and TP. However, on the abiotic substrata, biofilm properties greatly depended on the type of medium and substratum. In BHI, the viability of HAP-biofilm first decreased and then increased, whereas that of TI-biofilm decreased in time until a 6-log reduction. In TP, either no or a 2-log reduction in viability was observed for HAP- or TI-biofilms respectively. Furthermore, different bacterial genera (or higher level) were differentially abundant in the biofilms on 3 substrata: Haemophilus and Porphyromonas for RHG; Bacilli for HAP and Prevotella for TI. In conclusion, RHG, the biotic substratum, is able to support a highly viable and diverse microbiome. In contrast, the viability and diversity of the biofilms on the abiotic substrata were influenced by the substrata type, pH of the environment and the richness of the growth media. These results suggest that the host (oral mucosa) plays a vital role in the oral ecology.Xiaolan LiLin ShangBernd W. BrandtMark J. BuijsSanne RoffelCor van LoverenWim CrielaardSusan GibbsDong Mei DengNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Xiaolan Li Lin Shang Bernd W. Brandt Mark J. Buijs Sanne Roffel Cor van Loveren Wim Crielaard Susan Gibbs Dong Mei Deng Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
description	Abstract The microbial composition of a specific oral niche could be influenced by initial bacterial adherence, nutrient and physiological property of the local surface. To investigate the influence of nutrient and surface properties on microbial composition, saliva-derived biofilms were grown in agar on three substrata: Reconstructed Human Gingiva (RHG), a hydroxyapatite (HAP) surface, and a titanium (TI) surface. Agar was mixed with either Brain Heart Infusion (BHI) or Thompson (TP) medium. After 1, 3, or 5 days, biofilm viability (by colony forming units) and microbiome profiles (by 16 S rDNA amplicon sequencing) were determined. On RHG, biofilm viability and composition were similar between BHI and TP. However, on the abiotic substrata, biofilm properties greatly depended on the type of medium and substratum. In BHI, the viability of HAP-biofilm first decreased and then increased, whereas that of TI-biofilm decreased in time until a 6-log reduction. In TP, either no or a 2-log reduction in viability was observed for HAP- or TI-biofilms respectively. Furthermore, different bacterial genera (or higher level) were differentially abundant in the biofilms on 3 substrata: Haemophilus and Porphyromonas for RHG; Bacilli for HAP and Prevotella for TI. In conclusion, RHG, the biotic substratum, is able to support a highly viable and diverse microbiome. In contrast, the viability and diversity of the biofilms on the abiotic substrata were influenced by the substrata type, pH of the environment and the richness of the growth media. These results suggest that the host (oral mucosa) plays a vital role in the oral ecology.
format	article
author	Xiaolan Li Lin Shang Bernd W. Brandt Mark J. Buijs Sanne Roffel Cor van Loveren Wim Crielaard Susan Gibbs Dong Mei Deng
author_facet	Xiaolan Li Lin Shang Bernd W. Brandt Mark J. Buijs Sanne Roffel Cor van Loveren Wim Crielaard Susan Gibbs Dong Mei Deng
author_sort	Xiaolan Li
title	Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
title_short	Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
title_full	Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
title_fullStr	Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
title_full_unstemmed	Saliva-derived microcosm biofilms grown on different oral surfaces in vitro
title_sort	saliva-derived microcosm biofilms grown on different oral surfaces in vitro
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fe2857e0011f4bc0a23c327a7387c9bb
work_keys_str_mv	AT xiaolanli salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT linshang salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT berndwbrandt salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT markjbuijs salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT sanneroffel salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT corvanloveren salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT wimcrielaard salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT susangibbs salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro AT dongmeideng salivaderivedmicrocosmbiofilmsgrownondifferentoralsurfacesinvitro
_version_	1718381062700138496

Saliva-derived microcosm biofilms grown on different oral surfaces in vitro

Ejemplares similares