Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
Bread wheat (<i>Triticum</i> <i>aestivum</i> L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the <i>Fusarium</i> &l...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/fe28b652d7e047c18c7fbc926d1e0e55 |
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| Sumario: | Bread wheat (<i>Triticum</i> <i>aestivum</i> L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the <i>Fusarium</i> <i>culmorum</i> fungus. This pathogen is a causative agent of Fusarium root rot and Fusarium head blight. Beneficial fungi <i>Trichoderma atroviride</i> and <i>T. cremeum</i> are strong antagonists of mycotoxigenic <i>Fusarium</i> spp. These fungi promote plant growth and enhance their tolerance of negative environmental conditions. The aim of the study was to determine and compare the spatial (in above- and underground organs) and temporal (early: 6 and 22 hpi; and late: 5 and 7 dpi reactions) expression profiles of three mature miRNAs (miR398, miR167, and miR159) in wheat plants inoculated with two strains of <i>F. culmorum</i> (KF846 and EW49). Moreover, the spatial expression patterns in wheat response between plants inoculated with beneficial <i>T. atroviride</i> (AN35) and <i>T. cremeum</i> (AN392) were assessed. Understanding the sophisticated role of miRNAs in wheat–fungal interactions may initiate a discussion concerning the use of this knowledge to protect wheat plants from the harmful effects of fungal pathogens. With the use of droplet digital PCR (ddPCR), the absolute quantification of the selected miRNAs in the tested material was carried out. The differential accumulation of miR398, miR167, and miR159 in the studied groups was observed. The abundance of all analyzed miRNAs in the roots demonstrated an increase in the early and reduction in late wheat response to <i>F. culmorum</i> inoculation, suggesting the role of these particles in the initial wheat reaction to the studied fungal pathogen. The diverse expression patterns of the studied miRNAs between <i>Trichoderma</i>–inoculated or <i>F. culmorum</i>–inoculated plants and control wheat, as well as between <i>Trichoderma</i>–inoculated and <i>F. culmorum</i>–inoculated plants, were noticed, indicating the need for further analysis. |
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