Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (Triticum aestivum L.) and Pathogenic Fusarium culmorum and Beneficial Trichoderma Fungi

Bread wheat (Triticum aestivum L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the Fusarium &l...

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Autores principales:	Sylwia Salamon, Julia Żok, Karolina Gromadzka, Lidia Błaszczyk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	wheat plant–fungi interactions microRNA <i>Fusarium culmorum</i> <i>Trichoderma atroviride</i> <i>Trichoderma cremeum</i> Medicine R
Acceso en línea:	https://doaj.org/article/fe28b652d7e047c18c7fbc926d1e0e55
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spelling	oai:doaj.org-article:fe28b652d7e047c18c7fbc926d1e0e552021-11-25T18:38:26ZExpression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi10.3390/pathogens101114612076-0817https://doaj.org/article/fe28b652d7e047c18c7fbc926d1e0e552021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1461https://doaj.org/toc/2076-0817Bread wheat (<i>Triticum</i> <i>aestivum</i> L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the <i>Fusarium</i> <i>culmorum</i> fungus. This pathogen is a causative agent of Fusarium root rot and Fusarium head blight. Beneficial fungi <i>Trichoderma atroviride</i> and <i>T. cremeum</i> are strong antagonists of mycotoxigenic <i>Fusarium</i> spp. These fungi promote plant growth and enhance their tolerance of negative environmental conditions. The aim of the study was to determine and compare the spatial (in above- and underground organs) and temporal (early: 6 and 22 hpi; and late: 5 and 7 dpi reactions) expression profiles of three mature miRNAs (miR398, miR167, and miR159) in wheat plants inoculated with two strains of <i>F. culmorum</i> (KF846 and EW49). Moreover, the spatial expression patterns in wheat response between plants inoculated with beneficial <i>T. atroviride</i> (AN35) and <i>T. cremeum</i> (AN392) were assessed. Understanding the sophisticated role of miRNAs in wheat–fungal interactions may initiate a discussion concerning the use of this knowledge to protect wheat plants from the harmful effects of fungal pathogens. With the use of droplet digital PCR (ddPCR), the absolute quantification of the selected miRNAs in the tested material was carried out. The differential accumulation of miR398, miR167, and miR159 in the studied groups was observed. The abundance of all analyzed miRNAs in the roots demonstrated an increase in the early and reduction in late wheat response to <i>F. culmorum</i> inoculation, suggesting the role of these particles in the initial wheat reaction to the studied fungal pathogen. The diverse expression patterns of the studied miRNAs between <i>Trichoderma</i>–inoculated or <i>F. culmorum</i>–inoculated plants and control wheat, as well as between <i>Trichoderma</i>–inoculated and <i>F. culmorum</i>–inoculated plants, were noticed, indicating the need for further analysis.Sylwia SalamonJulia ŻokKarolina GromadzkaLidia BłaszczykMDPI AGarticlewheatplant–fungi interactionsmicroRNA<i>Fusarium culmorum</i><i>Trichoderma atroviride</i><i>Trichoderma cremeum</i>MedicineRENPathogens, Vol 10, Iss 1461, p 1461 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	wheat plant–fungi interactions microRNA <i>Fusarium culmorum</i> <i>Trichoderma atroviride</i> <i>Trichoderma cremeum</i> Medicine R
spellingShingle	wheat plant–fungi interactions microRNA <i>Fusarium culmorum</i> <i>Trichoderma atroviride</i> <i>Trichoderma cremeum</i> Medicine R Sylwia Salamon Julia Żok Karolina Gromadzka Lidia Błaszczyk Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
description	Bread wheat (<i>Triticum</i> <i>aestivum</i> L.) is an agronomically significant cereal cultivated worldwide. Wheat breeding is limited by numerous abiotic and biotic stresses. One of the most deleterious factors is biotic stress provoked by the <i>Fusarium</i> <i>culmorum</i> fungus. This pathogen is a causative agent of Fusarium root rot and Fusarium head blight. Beneficial fungi <i>Trichoderma atroviride</i> and <i>T. cremeum</i> are strong antagonists of mycotoxigenic <i>Fusarium</i> spp. These fungi promote plant growth and enhance their tolerance of negative environmental conditions. The aim of the study was to determine and compare the spatial (in above- and underground organs) and temporal (early: 6 and 22 hpi; and late: 5 and 7 dpi reactions) expression profiles of three mature miRNAs (miR398, miR167, and miR159) in wheat plants inoculated with two strains of <i>F. culmorum</i> (KF846 and EW49). Moreover, the spatial expression patterns in wheat response between plants inoculated with beneficial <i>T. atroviride</i> (AN35) and <i>T. cremeum</i> (AN392) were assessed. Understanding the sophisticated role of miRNAs in wheat–fungal interactions may initiate a discussion concerning the use of this knowledge to protect wheat plants from the harmful effects of fungal pathogens. With the use of droplet digital PCR (ddPCR), the absolute quantification of the selected miRNAs in the tested material was carried out. The differential accumulation of miR398, miR167, and miR159 in the studied groups was observed. The abundance of all analyzed miRNAs in the roots demonstrated an increase in the early and reduction in late wheat response to <i>F. culmorum</i> inoculation, suggesting the role of these particles in the initial wheat reaction to the studied fungal pathogen. The diverse expression patterns of the studied miRNAs between <i>Trichoderma</i>–inoculated or <i>F. culmorum</i>–inoculated plants and control wheat, as well as between <i>Trichoderma</i>–inoculated and <i>F. culmorum</i>–inoculated plants, were noticed, indicating the need for further analysis.
format	article
author	Sylwia Salamon Julia Żok Karolina Gromadzka Lidia Błaszczyk
author_facet	Sylwia Salamon Julia Żok Karolina Gromadzka Lidia Błaszczyk
author_sort	Sylwia Salamon
title	Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
title_short	Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
title_full	Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
title_fullStr	Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
title_full_unstemmed	Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi
title_sort	expression patterns of mir398, mir167, and mir159 in the interaction between bread wheat (<i>triticum</i> <i>aestivum</i> l.) and pathogenic <i>fusarium</i> <i>culmorum</i> and beneficial <i>trichoderma</i> fungi
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fe28b652d7e047c18c7fbc926d1e0e55
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Expression Patterns of miR398, miR167, and miR159 in the Interaction between Bread Wheat (<i>Triticum</i> <i>aestivum</i> L.) and Pathogenic <i>Fusarium</i> <i>culmorum</i> and Beneficial <i>Trichoderma</i> Fungi

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