Optical mapping of neuronal activity during seizures in zebrafish

Abstract Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs. Recently, zebrafish has become an important model for studying epilepsy...

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Autores principales:	L. Turrini, C. Fornetto, G. Marchetto, M. C. Müllenbroich, N. Tiso, A. Vettori, F. Resta, A. Masi, G. Mannaioni, F. S. Pavone, F. Vanzi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe41da28be28404f85eedb221b900c22
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spelling	oai:doaj.org-article:fe41da28be28404f85eedb221b900c222021-12-02T16:07:59ZOptical mapping of neuronal activity during seizures in zebrafish10.1038/s41598-017-03087-z2045-2322https://doaj.org/article/fe41da28be28404f85eedb221b900c222017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03087-zhttps://doaj.org/toc/2045-2322Abstract Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs. Recently, zebrafish has become an important model for studying epilepsy both in basic research and in drug discovery. Here, we employed a transgenic line with pan-neuronal expression of the genetically-encoded calcium indicator GCaMP6s to measure neuronal activity in zebrafish larvae during seizures induced by pentylenetretrazole (PTZ). With this approach, we mapped neuronal activity in different areas of the larval brain, demonstrating the high sensitivity of this method to different levels of alteration, as induced by increasing PTZ concentrations, and the rescuing effect of an anti-epileptic drug. We also present simultaneous measurements of brain and locomotor activity, as well as a high-throughput assay, demonstrating that GCaMP measurements can complement behavioural assays for the detection of subclinical epileptic seizures, thus enabling future investigations on human hypomorphic mutations and more effective drug screening methods. Notably, the methodology described here can be easily applied to the study of many human neuropathologies modelled in zebrafish, allowing a simple and yet detailed investigation of brain activity alterations associated with the pathological phenotype.L. TurriniC. FornettoG. MarchettoM. C. MüllenbroichN. TisoA. VettoriF. RestaA. MasiG. MannaioniF. S. PavoneF. VanziNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. Turrini C. Fornetto G. Marchetto M. C. Müllenbroich N. Tiso A. Vettori F. Resta A. Masi G. Mannaioni F. S. Pavone F. Vanzi Optical mapping of neuronal activity during seizures in zebrafish
description	Abstract Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs. Recently, zebrafish has become an important model for studying epilepsy both in basic research and in drug discovery. Here, we employed a transgenic line with pan-neuronal expression of the genetically-encoded calcium indicator GCaMP6s to measure neuronal activity in zebrafish larvae during seizures induced by pentylenetretrazole (PTZ). With this approach, we mapped neuronal activity in different areas of the larval brain, demonstrating the high sensitivity of this method to different levels of alteration, as induced by increasing PTZ concentrations, and the rescuing effect of an anti-epileptic drug. We also present simultaneous measurements of brain and locomotor activity, as well as a high-throughput assay, demonstrating that GCaMP measurements can complement behavioural assays for the detection of subclinical epileptic seizures, thus enabling future investigations on human hypomorphic mutations and more effective drug screening methods. Notably, the methodology described here can be easily applied to the study of many human neuropathologies modelled in zebrafish, allowing a simple and yet detailed investigation of brain activity alterations associated with the pathological phenotype.
format	article
author	L. Turrini C. Fornetto G. Marchetto M. C. Müllenbroich N. Tiso A. Vettori F. Resta A. Masi G. Mannaioni F. S. Pavone F. Vanzi
author_facet	L. Turrini C. Fornetto G. Marchetto M. C. Müllenbroich N. Tiso A. Vettori F. Resta A. Masi G. Mannaioni F. S. Pavone F. Vanzi
author_sort	L. Turrini
title	Optical mapping of neuronal activity during seizures in zebrafish
title_short	Optical mapping of neuronal activity during seizures in zebrafish
title_full	Optical mapping of neuronal activity during seizures in zebrafish
title_fullStr	Optical mapping of neuronal activity during seizures in zebrafish
title_full_unstemmed	Optical mapping of neuronal activity during seizures in zebrafish
title_sort	optical mapping of neuronal activity during seizures in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fe41da28be28404f85eedb221b900c22
work_keys_str_mv	AT lturrini opticalmappingofneuronalactivityduringseizuresinzebrafish AT cfornetto opticalmappingofneuronalactivityduringseizuresinzebrafish AT gmarchetto opticalmappingofneuronalactivityduringseizuresinzebrafish AT mcmullenbroich opticalmappingofneuronalactivityduringseizuresinzebrafish AT ntiso opticalmappingofneuronalactivityduringseizuresinzebrafish AT avettori opticalmappingofneuronalactivityduringseizuresinzebrafish AT fresta opticalmappingofneuronalactivityduringseizuresinzebrafish AT amasi opticalmappingofneuronalactivityduringseizuresinzebrafish AT gmannaioni opticalmappingofneuronalactivityduringseizuresinzebrafish AT fspavone opticalmappingofneuronalactivityduringseizuresinzebrafish AT fvanzi opticalmappingofneuronalactivityduringseizuresinzebrafish
_version_	1718384694753492992

Optical mapping of neuronal activity during seizures in zebrafish

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