DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis

Abstract Background Septic arthritis is a worse condition of RA that is associated with significant morbidity and mortality. Septic arthritis develops due to direct introduction or invasion of pathogens. The objective of the present study was to formulate Vancomycin hydrochloride-loaded microspheres...

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Autores principales:	P. Sunanda Laxmi, M. Vidyavathi, Suresh Kumar Rayadurgam Venkata
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Vancomycin Microspheres Arthritis Sepsis Micromeritics Intraarticular injection Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/fe49d2c334a64051ade4aeafabf87c53
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spelling	oai:doaj.org-article:fe49d2c334a64051ade4aeafabf87c532021-12-05T12:21:01ZDoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis10.1186/s43094-021-00382-52314-7253https://doaj.org/article/fe49d2c334a64051ade4aeafabf87c532021-12-01T00:00:00Zhttps://doi.org/10.1186/s43094-021-00382-5https://doaj.org/toc/2314-7253Abstract Background Septic arthritis is a worse condition of RA that is associated with significant morbidity and mortality. Septic arthritis develops due to direct introduction or invasion of pathogens. The objective of the present study was to formulate Vancomycin hydrochloride-loaded microspheres (VMS) based on Box–Behnken design (BBD) and evaluate its efficacy against septic arthritis. The intraarticular administration of optimized Vancomycin hydrochloride-loaded microspheres (OVMS) can reduce dose size, dosing frequency and systemic exposure with local targeted delivery. Results OVMS was further characterized for its drug–polymer compatibility using differential scanning calorimetry and Fourier transmission infrared spectroscopy. In vitro antibacterial activity was determined using the cup–plate method and in vivo anti-arthritic efficacy was evaluated by gross examination of septic arthritis. DSC and FTIR studies exhibited no interaction or incompatibilities between the drug and polymer. SEM images revealed that OVMS were spherical. It followed the first-order release rate according to Fick's law. The micromeritic properties indicated good flow property of OVMS. The zone of inhibition by OVMS was 1.5 cm against S. aureus. In vivo antibacterial study revealed that OVMS was significant in reducing septic arthritis and bacterial load, i.e., 110.1 CFU/ml in comparison with the control group (850 CFU/ml). Conclusions Thus, OVMS may be used as an effective formulation for the treatment of septic arthritis as compared to marketed IV vancomycin injection after clinical studies.P. Sunanda LaxmiM. VidyavathiSuresh Kumar Rayadurgam VenkataSpringerOpenarticleVancomycinMicrospheresArthritisSepsisMicromeriticsIntraarticular injectionTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441ENFuture Journal of Pharmaceutical Sciences, Vol 7, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vancomycin Microspheres Arthritis Sepsis Micromeritics Intraarticular injection Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	Vancomycin Microspheres Arthritis Sepsis Micromeritics Intraarticular injection Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 P. Sunanda Laxmi M. Vidyavathi Suresh Kumar Rayadurgam Venkata DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
description	Abstract Background Septic arthritis is a worse condition of RA that is associated with significant morbidity and mortality. Septic arthritis develops due to direct introduction or invasion of pathogens. The objective of the present study was to formulate Vancomycin hydrochloride-loaded microspheres (VMS) based on Box–Behnken design (BBD) and evaluate its efficacy against septic arthritis. The intraarticular administration of optimized Vancomycin hydrochloride-loaded microspheres (OVMS) can reduce dose size, dosing frequency and systemic exposure with local targeted delivery. Results OVMS was further characterized for its drug–polymer compatibility using differential scanning calorimetry and Fourier transmission infrared spectroscopy. In vitro antibacterial activity was determined using the cup–plate method and in vivo anti-arthritic efficacy was evaluated by gross examination of septic arthritis. DSC and FTIR studies exhibited no interaction or incompatibilities between the drug and polymer. SEM images revealed that OVMS were spherical. It followed the first-order release rate according to Fick's law. The micromeritic properties indicated good flow property of OVMS. The zone of inhibition by OVMS was 1.5 cm against S. aureus. In vivo antibacterial study revealed that OVMS was significant in reducing septic arthritis and bacterial load, i.e., 110.1 CFU/ml in comparison with the control group (850 CFU/ml). Conclusions Thus, OVMS may be used as an effective formulation for the treatment of septic arthritis as compared to marketed IV vancomycin injection after clinical studies.
format	article
author	P. Sunanda Laxmi M. Vidyavathi Suresh Kumar Rayadurgam Venkata
author_facet	P. Sunanda Laxmi M. Vidyavathi Suresh Kumar Rayadurgam Venkata
author_sort	P. Sunanda Laxmi
title	DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
title_short	DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
title_full	DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
title_fullStr	DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
title_full_unstemmed	DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis
title_sort	doe approach for development of localized controlled release microspheres of vancomycin for treatment of septic arthritis
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/fe49d2c334a64051ade4aeafabf87c53
work_keys_str_mv	AT psunandalaxmi doeapproachfordevelopmentoflocalizedcontrolledreleasemicrospheresofvancomycinfortreatmentofsepticarthritis AT mvidyavathi doeapproachfordevelopmentoflocalizedcontrolledreleasemicrospheresofvancomycinfortreatmentofsepticarthritis AT sureshkumarrayadurgamvenkata doeapproachfordevelopmentoflocalizedcontrolledreleasemicrospheresofvancomycinfortreatmentofsepticarthritis
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DoE approach for development of localized controlled release microspheres of Vancomycin for treatment of septic arthritis

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