PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES

Present study used in vitro cultures of human peripheral blood cells (PBC) with addition of γ-globulins, or their complexes with copper and zinc, or single copper and zinc ions. We have shown that, among common pool of induced cytokines, interleukin-18 (IL-18) was detectabl...

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Autores principales:	S. B. Cheknev, M. A. Apresova, I. E. Efremova, A. A. Babajanz
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	γ-globulin metal complexes il-18 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fe4b21a56d404d4ab5131678ed80e156
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spelling	oai:doaj.org-article:fe4b21a56d404d4ab5131678ed80e1562021-11-18T08:03:37ZPRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES1563-06252313-741X10.15789/1563-0625-2013-1-13-20https://doaj.org/article/fe4b21a56d404d4ab5131678ed80e1562014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/54https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XPresent study used in vitro cultures of human peripheral blood cells (PBC) with addition of γ-globulins, or their complexes with copper and zinc, or single copper and zinc ions. We have shown that, among common pool of induced cytokines, interleukin-18 (IL-18) was detectable at a concentration of 24.4±1.03 to 33.25±0.90 pg/ml. IL-18 production in PBC after 24h treatment with γ-globulin-zinc complexes zinc was higher by 13-19% than following 24-h incubation with control proteins, or zinc ions. Accordingly, IL-18 production in PBC after 72-h incubation with γ-globulin copper complexes was higher than with control proteins. In parallel to IL-18, human PBC produce detectable interferon (IFN, up to 32 U/ml). We have shown a strong positive correlation between the peaks of IFN increase, and elevated IL-18 levels caused by γ-globulin-metal complexes. Hence, an opportunity for regulation of intracellular IL-18 and IFN production by γ-globulin-metal complexes at the level of basal cell functions was demonstrated in context of IFNγ induction, thus causing Th1-directed shift of immune response.S. B. CheknevM. A. ApresovaI. E. EfremovaA. A. BabajanzSPb RAACIarticleγ-globulinmetal complexesil-18Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 15, Iss 1, Pp 13-20 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	γ-globulin metal complexes il-18 Immunologic diseases. Allergy RC581-607
spellingShingle	γ-globulin metal complexes il-18 Immunologic diseases. Allergy RC581-607 S. B. Cheknev M. A. Apresova I. E. Efremova A. A. Babajanz PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
description	Present study used in vitro cultures of human peripheral blood cells (PBC) with addition of γ-globulins, or their complexes with copper and zinc, or single copper and zinc ions. We have shown that, among common pool of induced cytokines, interleukin-18 (IL-18) was detectable at a concentration of 24.4±1.03 to 33.25±0.90 pg/ml. IL-18 production in PBC after 24h treatment with γ-globulin-zinc complexes zinc was higher by 13-19% than following 24-h incubation with control proteins, or zinc ions. Accordingly, IL-18 production in PBC after 72-h incubation with γ-globulin copper complexes was higher than with control proteins. In parallel to IL-18, human PBC produce detectable interferon (IFN, up to 32 U/ml). We have shown a strong positive correlation between the peaks of IFN increase, and elevated IL-18 levels caused by γ-globulin-metal complexes. Hence, an opportunity for regulation of intracellular IL-18 and IFN production by γ-globulin-metal complexes at the level of basal cell functions was demonstrated in context of IFNγ induction, thus causing Th1-directed shift of immune response.
format	article
author	S. B. Cheknev M. A. Apresova I. E. Efremova A. A. Babajanz
author_facet	S. B. Cheknev M. A. Apresova I. E. Efremova A. A. Babajanz
author_sort	S. B. Cheknev
title	PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
title_short	PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
title_full	PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
title_fullStr	PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
title_full_unstemmed	PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES
title_sort	production of il-18 in presence of metal-γ-globulin complexes
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/fe4b21a56d404d4ab5131678ed80e156
work_keys_str_mv	AT sbcheknev productionofil18inpresenceofmetalgglobulincomplexes AT maapresova productionofil18inpresenceofmetalgglobulincomplexes AT ieefremova productionofil18inpresenceofmetalgglobulincomplexes AT aababajanz productionofil18inpresenceofmetalgglobulincomplexes
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PRODUCTION OF IL-18 IN PRESENCE OF METAL-γ-GLOBULIN COMPLEXES

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