Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India

Abstract Alternaria species produce various sorts of toxic metabolites during their active growth and causes severe diseases in many plants by limiting their productivity. These toxic metabolites incorporate various mycotoxins comprising of dibenzo-α-pyrone and some tetramic acid derivatives. In thi...

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Autores principales:	Mukesh Meena, Prashant Swapnil, R. S. Upadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe4c56c729194bd99beee1c4f1ed0605
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spelling	oai:doaj.org-article:fe4c56c729194bd99beee1c4f1ed06052021-12-02T12:32:31ZIsolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India10.1038/s41598-017-09138-92045-2322https://doaj.org/article/fe4c56c729194bd99beee1c4f1ed06052017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09138-9https://doaj.org/toc/2045-2322Abstract Alternaria species produce various sorts of toxic metabolites during their active growth and causes severe diseases in many plants by limiting their productivity. These toxic metabolites incorporate various mycotoxins comprising of dibenzo-α-pyrone and some tetramic acid derivatives. In this study, we have screened out total 48 isolates of Alternaria from different plants belonging to different locations in India, on the basis of their pathogenic nature. Pathogenicity testing of these 48 strains on susceptible tomato variety (CO-3) showed 27.08% of the strains were highly pathogenic, 35.41% moderately pathogenic and 37.5% were less pathogenic. Phylogenetic analysis showed the presence of at least eight evolutionary cluster of the pathogen. Toxins (TeA, AOH and AME) were isolated, purified on the basis of column chromatography and TLC, and further confirmed by the HPLC-UV chromatograms using standards. The final detection of toxins was done by the LC-MS/MS analysis by their mass/charge ratio. The present study develops an approach to classify the toxicogenic effect of each of the individual mycotoxins on tomato plant and focuses their differential susceptibility to develop disease symptoms. This study represents the report of the natural occurrence and distribution of Alternaria toxins in various plants from India.Mukesh MeenaPrashant SwapnilR. S. UpadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-19 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mukesh Meena Prashant Swapnil R. S. Upadhyay Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
description	Abstract Alternaria species produce various sorts of toxic metabolites during their active growth and causes severe diseases in many plants by limiting their productivity. These toxic metabolites incorporate various mycotoxins comprising of dibenzo-α-pyrone and some tetramic acid derivatives. In this study, we have screened out total 48 isolates of Alternaria from different plants belonging to different locations in India, on the basis of their pathogenic nature. Pathogenicity testing of these 48 strains on susceptible tomato variety (CO-3) showed 27.08% of the strains were highly pathogenic, 35.41% moderately pathogenic and 37.5% were less pathogenic. Phylogenetic analysis showed the presence of at least eight evolutionary cluster of the pathogen. Toxins (TeA, AOH and AME) were isolated, purified on the basis of column chromatography and TLC, and further confirmed by the HPLC-UV chromatograms using standards. The final detection of toxins was done by the LC-MS/MS analysis by their mass/charge ratio. The present study develops an approach to classify the toxicogenic effect of each of the individual mycotoxins on tomato plant and focuses their differential susceptibility to develop disease symptoms. This study represents the report of the natural occurrence and distribution of Alternaria toxins in various plants from India.
format	article
author	Mukesh Meena Prashant Swapnil R. S. Upadhyay
author_facet	Mukesh Meena Prashant Swapnil R. S. Upadhyay
author_sort	Mukesh Meena
title	Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
title_short	Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
title_full	Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
title_fullStr	Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
title_full_unstemmed	Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India
title_sort	isolation, characterization and toxicological potential of alternaria-mycotoxins (tea, aoh and ame) in different alternaria species from various regions of india
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fe4c56c729194bd99beee1c4f1ed0605
work_keys_str_mv	AT mukeshmeena isolationcharacterizationandtoxicologicalpotentialofalternariamycotoxinsteaaohandameindifferentalternariaspeciesfromvariousregionsofindia AT prashantswapnil isolationcharacterizationandtoxicologicalpotentialofalternariamycotoxinsteaaohandameindifferentalternariaspeciesfromvariousregionsofindia AT rsupadhyay isolationcharacterizationandtoxicologicalpotentialofalternariamycotoxinsteaaohandameindifferentalternariaspeciesfromvariousregionsofindia
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Isolation, characterization and toxicological potential of Alternaria-mycotoxins (TeA, AOH and AME) in different Alternaria species from various regions of India

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