Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Wen-tao Lin,1 Hong-lue Tan,1 Zhao-ling Duan,2 Bing Yue,1 Rui Ma,1 Guo He,2 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lin WT, Tan HL, Duan ZL, Yue B, Ma R, He G, Tang TT
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fe4dff2d30bc49f6a7ad13348a008509
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe4dff2d30bc49f6a7ad13348a008509
record_format	dspace
spelling	oai:doaj.org-article:fe4dff2d30bc49f6a7ad13348a0085092021-12-02T02:02:32ZInhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters1178-2013https://doaj.org/article/fe4dff2d30bc49f6a7ad13348a0085092014-03-01T00:00:00Zhttp://www.dovepress.com/inhibited-bacterial-biofilm-formation-and-improved-osteogenic-activity-a16038https://doaj.org/toc/1178-2013 Wen-tao Lin,1 Hong-lue Tan,1 Zhao-ling Duan,2 Bing Yue,1 Rui Ma,1 Guo He,2 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; 2State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China Abstract: Titania nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specific site, thereby providing a promising strategy to prevent implant-associated infections. In this study we have fabricated titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length via electrochemical anodization. These nanotubes were loaded with 2 mg of gentamicin using a lyophilization method and vacuum drying. A standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the anti-infective ability of the gentamicin-loaded nanotubes (NT-G). Flat titanium (FlatTi) and nanotubes with no drug loading (NT) were also investigated and compared. We found that NT-G could significantly inhibit bacterial adhesion and biofilm formation compared to FlatTi or NT, and the NT-G with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended drug release time of NT-G with larger diameters. The NT also exhibited greater antibacterial ability than the FlatTi, while nanotubes with 80 nm or 120 nm diameters had better effects. Furthermore, human marrow derived mesenchymal stem cells were used to evaluate the effect of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells. Our results showed that NT-G and NT, especially those with 80 nm diameters, significantly promoted cell attachment, proliferation, spreading, and osteogenic differentiation when compared to FlatTi, and there was no significant difference between NT-G and NT with the same diameter. Therefore, nanotube modification and gentamicin loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants. Keywords: titania nanotubes, gentamicin, bacteria adhesion, biofilm formation, osteogenic activityLin WTTan HLDuan ZLYue BMa RHe GTang TTDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1215-1230 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lin WT Tan HL Duan ZL Yue B Ma R He G Tang TT Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
description	Wen-tao Lin,1 Hong-lue Tan,1 Zhao-ling Duan,2 Bing Yue,1 Rui Ma,1 Guo He,2 Ting-ting Tang1 1Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China; 2State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China Abstract: Titania nanotubes loaded with antibiotics can deliver a high concentration of antibiotics locally at a specific site, thereby providing a promising strategy to prevent implant-associated infections. In this study we have fabricated titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length via electrochemical anodization. These nanotubes were loaded with 2 mg of gentamicin using a lyophilization method and vacuum drying. A standard strain, Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the anti-infective ability of the gentamicin-loaded nanotubes (NT-G). Flat titanium (FlatTi) and nanotubes with no drug loading (NT) were also investigated and compared. We found that NT-G could significantly inhibit bacterial adhesion and biofilm formation compared to FlatTi or NT, and the NT-G with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended drug release time of NT-G with larger diameters. The NT also exhibited greater antibacterial ability than the FlatTi, while nanotubes with 80 nm or 120 nm diameters had better effects. Furthermore, human marrow derived mesenchymal stem cells were used to evaluate the effect of nanotubular topographies on the osteogenic differentiation of mesenchymal stem cells. Our results showed that NT-G and NT, especially those with 80 nm diameters, significantly promoted cell attachment, proliferation, spreading, and osteogenic differentiation when compared to FlatTi, and there was no significant difference between NT-G and NT with the same diameter. Therefore, nanotube modification and gentamicin loading can significantly improve the antibacterial ability and osteogenic activity of orthopedic implants. Keywords: titania nanotubes, gentamicin, bacteria adhesion, biofilm formation, osteogenic activity
format	article
author	Lin WT Tan HL Duan ZL Yue B Ma R He G Tang TT
author_facet	Lin WT Tan HL Duan ZL Yue B Ma R He G Tang TT
author_sort	Lin WT
title	Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
title_short	Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
title_full	Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
title_fullStr	Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
title_full_unstemmed	Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
title_sort	inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/fe4dff2d30bc49f6a7ad13348a008509
work_keys_str_mv	AT linwt inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT tanhl inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT duanzl inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT yueb inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT mar inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT heg inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters AT tangtt inhibitedbacterialbiofilmformationandimprovedosteogenicactivityongentamicinloadedtitaniananotubeswithvariousdiameters
_version_	1718402751819415552

Inhibited bacterial biofilm formation and improved osteogenic activity on gentamicin-loaded titania nanotubes with various diameters

Ejemplares similares