The Bacterial Biofilm Matrix as a Platform for Protein Delivery

ABSTRACT Surface-associated bacterial structures known as biofilms are the target of intense antimicrobial research efforts. We recently identified several secreted proteins that are retained in the bacterial biofilm matrix by their association with the biofilm exopolysaccharide scaffold. Based on o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cedric Absalon, Patrick Ymele-Leki, Paula I. Watnick
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2012
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fe51bb38eb834099ba1d14c4a8a54227
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe51bb38eb834099ba1d14c4a8a54227
record_format	dspace
spelling	oai:doaj.org-article:fe51bb38eb834099ba1d14c4a8a542272021-11-15T15:39:10ZThe Bacterial Biofilm Matrix as a Platform for Protein Delivery10.1128/mBio.00127-122150-7511https://doaj.org/article/fe51bb38eb834099ba1d14c4a8a542272012-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00127-12https://doaj.org/toc/2150-7511ABSTRACT Surface-associated bacterial structures known as biofilms are the target of intense antimicrobial research efforts. We recently identified several secreted proteins that are retained in the bacterial biofilm matrix by their association with the biofilm exopolysaccharide scaffold. Based on our findings, we hypothesized that these problematic bacterial structures might be reengineered to serve as reservoirs for surface-active secreted proteins of biomedical, bioengineering, or biotechnological importance. By piggybacking onto one of these scaffold-associated proteins, we were able to sequester a functional enzyme to the biofilm matrix. We hypothesize that this technology may have diverse applications in vaccine design, digestive disease, and bioremediation.Cedric AbsalonPatrick Ymele-LekiPaula I. WatnickAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 3, Iss 4 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Cedric Absalon Patrick Ymele-Leki Paula I. Watnick The Bacterial Biofilm Matrix as a Platform for Protein Delivery
description	ABSTRACT Surface-associated bacterial structures known as biofilms are the target of intense antimicrobial research efforts. We recently identified several secreted proteins that are retained in the bacterial biofilm matrix by their association with the biofilm exopolysaccharide scaffold. Based on our findings, we hypothesized that these problematic bacterial structures might be reengineered to serve as reservoirs for surface-active secreted proteins of biomedical, bioengineering, or biotechnological importance. By piggybacking onto one of these scaffold-associated proteins, we were able to sequester a functional enzyme to the biofilm matrix. We hypothesize that this technology may have diverse applications in vaccine design, digestive disease, and bioremediation.
format	article
author	Cedric Absalon Patrick Ymele-Leki Paula I. Watnick
author_facet	Cedric Absalon Patrick Ymele-Leki Paula I. Watnick
author_sort	Cedric Absalon
title	The Bacterial Biofilm Matrix as a Platform for Protein Delivery
title_short	The Bacterial Biofilm Matrix as a Platform for Protein Delivery
title_full	The Bacterial Biofilm Matrix as a Platform for Protein Delivery
title_fullStr	The Bacterial Biofilm Matrix as a Platform for Protein Delivery
title_full_unstemmed	The Bacterial Biofilm Matrix as a Platform for Protein Delivery
title_sort	bacterial biofilm matrix as a platform for protein delivery
publisher	American Society for Microbiology
publishDate	2012
url	https://doaj.org/article/fe51bb38eb834099ba1d14c4a8a54227
work_keys_str_mv	AT cedricabsalon thebacterialbiofilmmatrixasaplatformforproteindelivery AT patrickymeleleki thebacterialbiofilmmatrixasaplatformforproteindelivery AT paulaiwatnick thebacterialbiofilmmatrixasaplatformforproteindelivery AT cedricabsalon bacterialbiofilmmatrixasaplatformforproteindelivery AT patrickymeleleki bacterialbiofilmmatrixasaplatformforproteindelivery AT paulaiwatnick bacterialbiofilmmatrixasaplatformforproteindelivery
_version_	1718427804958195712

The Bacterial Biofilm Matrix as a Platform for Protein Delivery

Ejemplares similares