Microtubule disruption changes endothelial cell mechanics and adhesion

Abstract The interest in studying the mechanical and adhesive properties of cells has increased in recent years. The cytoskeleton is known to play a key role in cell mechanics. However, the role of the microtubules in shaping cell mechanics is not yet well understood. We have employed Atomic Force M...

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Autores principales:	Andreas Weber, Jagoba Iturri, Rafael Benitez, Spela Zemljic-Jokhadar, José L. Toca-Herrera
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe5caa122b88497798f54afea2ec8a10
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spelling	oai:doaj.org-article:fe5caa122b88497798f54afea2ec8a102021-12-02T16:08:43ZMicrotubule disruption changes endothelial cell mechanics and adhesion10.1038/s41598-019-51024-z2045-2322https://doaj.org/article/fe5caa122b88497798f54afea2ec8a102019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-51024-zhttps://doaj.org/toc/2045-2322Abstract The interest in studying the mechanical and adhesive properties of cells has increased in recent years. The cytoskeleton is known to play a key role in cell mechanics. However, the role of the microtubules in shaping cell mechanics is not yet well understood. We have employed Atomic Force Microscopy (AFM) together with confocal fluorescence microscopy to determine the role of microtubules in cytomechanics of Human Umbilical Vein Endothelial Cells (HUVECs). Additionally, the time variation of the adhesion between tip and cell surface was studied. The disruption of microtubules by exposing the cells to two colchicine concentrations was monitored as a function of time. Already, after 30 min of incubation the cells stiffened, their relaxation times increased (lower fluidity) and the adhesion between tip and cell decreased. This was accompanied by cytoskeletal rearrangements, a reduction in cell area and changes in cell shape. Over the whole experimental time, different behavior for the two used concentrations was found while for the control the values remained stable. This study underlines the role of microtubules in shaping endothelial cell mechanics.Andreas WeberJagoba IturriRafael BenitezSpela Zemljic-JokhadarJosé L. Toca-HerreraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreas Weber Jagoba Iturri Rafael Benitez Spela Zemljic-Jokhadar José L. Toca-Herrera Microtubule disruption changes endothelial cell mechanics and adhesion
description	Abstract The interest in studying the mechanical and adhesive properties of cells has increased in recent years. The cytoskeleton is known to play a key role in cell mechanics. However, the role of the microtubules in shaping cell mechanics is not yet well understood. We have employed Atomic Force Microscopy (AFM) together with confocal fluorescence microscopy to determine the role of microtubules in cytomechanics of Human Umbilical Vein Endothelial Cells (HUVECs). Additionally, the time variation of the adhesion between tip and cell surface was studied. The disruption of microtubules by exposing the cells to two colchicine concentrations was monitored as a function of time. Already, after 30 min of incubation the cells stiffened, their relaxation times increased (lower fluidity) and the adhesion between tip and cell decreased. This was accompanied by cytoskeletal rearrangements, a reduction in cell area and changes in cell shape. Over the whole experimental time, different behavior for the two used concentrations was found while for the control the values remained stable. This study underlines the role of microtubules in shaping endothelial cell mechanics.
format	article
author	Andreas Weber Jagoba Iturri Rafael Benitez Spela Zemljic-Jokhadar José L. Toca-Herrera
author_facet	Andreas Weber Jagoba Iturri Rafael Benitez Spela Zemljic-Jokhadar José L. Toca-Herrera
author_sort	Andreas Weber
title	Microtubule disruption changes endothelial cell mechanics and adhesion
title_short	Microtubule disruption changes endothelial cell mechanics and adhesion
title_full	Microtubule disruption changes endothelial cell mechanics and adhesion
title_fullStr	Microtubule disruption changes endothelial cell mechanics and adhesion
title_full_unstemmed	Microtubule disruption changes endothelial cell mechanics and adhesion
title_sort	microtubule disruption changes endothelial cell mechanics and adhesion
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/fe5caa122b88497798f54afea2ec8a10
work_keys_str_mv	AT andreasweber microtubuledisruptionchangesendothelialcellmechanicsandadhesion AT jagobaiturri microtubuledisruptionchangesendothelialcellmechanicsandadhesion AT rafaelbenitez microtubuledisruptionchangesendothelialcellmechanicsandadhesion AT spelazemljicjokhadar microtubuledisruptionchangesendothelialcellmechanicsandadhesion AT joseltocaherrera microtubuledisruptionchangesendothelialcellmechanicsandadhesion
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Microtubule disruption changes endothelial cell mechanics and adhesion

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