Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.

Burkholderia glumae is a motile plant pathogenic bacterium that has multiple polar flagella and one LuxR/LuxI-type quorum sensing (QS) system, TofR/TofI. A QS-dependent transcriptional regulator, QsmR, activates flagellar master regulator flhDC genes. FlhDC subsequently activates flagellar gene expr...

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Autores principales:	Moon Sun Jang, Eunhye Goo, Jae Hyung An, Jinwoo Kim, Ingyu Hwang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe61542051cd4dac8647b0c6304afb90
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spelling	oai:doaj.org-article:fe61542051cd4dac8647b0c6304afb902021-11-18T08:38:29ZQuorum sensing controls flagellar morphogenesis in Burkholderia glumae.1932-620310.1371/journal.pone.0084831https://doaj.org/article/fe61542051cd4dac8647b0c6304afb902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416296/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Burkholderia glumae is a motile plant pathogenic bacterium that has multiple polar flagella and one LuxR/LuxI-type quorum sensing (QS) system, TofR/TofI. A QS-dependent transcriptional regulator, QsmR, activates flagellar master regulator flhDC genes. FlhDC subsequently activates flagellar gene expression in B. glumae at 37°C. Here, we confirm that the interplay between QS and temperature is critical for normal polar flagellar morphogenesis in B. glumae. In the wild-type bacterium, flagellar gene expression and flagellar number were greater at 28°C compared to 37°C. The QS-dependent flhC gene was significantly expressed at 28°C in two QS-defective (tofI::Ω and qsmR::Ω) mutants. Thus, flagella were present in both tofI::Ω and qsmR::Ω mutants at 28°C, but were absent at 37°C. Most tofI::Ω and qsmR::Ω mutant cells possessed polar or nonpolar flagella at 28°C. Nonpolarly flagellated cells processing flagella around cell surface of both tofI::Ω and qsmR::Ω mutants exhibited tumbling and spinning movements. The flhF gene encoding GTPase involved in regulating the correct placement of flagella in other bacteria was expressed in QS mutants in a FlhDC-dependent manner at 28°C. However, FlhF was mislocalized in QS mutants, and was associated with nonpolar flagellar formation in QS mutants at 28°C. These results indicate that QS-independent expression of flagellar genes at 28°C allows flagellar biogenesis, but is not sufficient for normal polar flagellar morphogenesis in B. glumae. Our findings demonstrate that QS functions together with temperature to control flagellar morphogenesis in B. glumae.Moon Sun JangEunhye GooJae Hyung AnJinwoo KimIngyu HwangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84831 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moon Sun Jang Eunhye Goo Jae Hyung An Jinwoo Kim Ingyu Hwang Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
description	Burkholderia glumae is a motile plant pathogenic bacterium that has multiple polar flagella and one LuxR/LuxI-type quorum sensing (QS) system, TofR/TofI. A QS-dependent transcriptional regulator, QsmR, activates flagellar master regulator flhDC genes. FlhDC subsequently activates flagellar gene expression in B. glumae at 37°C. Here, we confirm that the interplay between QS and temperature is critical for normal polar flagellar morphogenesis in B. glumae. In the wild-type bacterium, flagellar gene expression and flagellar number were greater at 28°C compared to 37°C. The QS-dependent flhC gene was significantly expressed at 28°C in two QS-defective (tofI::Ω and qsmR::Ω) mutants. Thus, flagella were present in both tofI::Ω and qsmR::Ω mutants at 28°C, but were absent at 37°C. Most tofI::Ω and qsmR::Ω mutant cells possessed polar or nonpolar flagella at 28°C. Nonpolarly flagellated cells processing flagella around cell surface of both tofI::Ω and qsmR::Ω mutants exhibited tumbling and spinning movements. The flhF gene encoding GTPase involved in regulating the correct placement of flagella in other bacteria was expressed in QS mutants in a FlhDC-dependent manner at 28°C. However, FlhF was mislocalized in QS mutants, and was associated with nonpolar flagellar formation in QS mutants at 28°C. These results indicate that QS-independent expression of flagellar genes at 28°C allows flagellar biogenesis, but is not sufficient for normal polar flagellar morphogenesis in B. glumae. Our findings demonstrate that QS functions together with temperature to control flagellar morphogenesis in B. glumae.
format	article
author	Moon Sun Jang Eunhye Goo Jae Hyung An Jinwoo Kim Ingyu Hwang
author_facet	Moon Sun Jang Eunhye Goo Jae Hyung An Jinwoo Kim Ingyu Hwang
author_sort	Moon Sun Jang
title	Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
title_short	Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
title_full	Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
title_fullStr	Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
title_full_unstemmed	Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.
title_sort	quorum sensing controls flagellar morphogenesis in burkholderia glumae.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fe61542051cd4dac8647b0c6304afb90
work_keys_str_mv	AT moonsunjang quorumsensingcontrolsflagellarmorphogenesisinburkholderiaglumae AT eunhyegoo quorumsensingcontrolsflagellarmorphogenesisinburkholderiaglumae AT jaehyungan quorumsensingcontrolsflagellarmorphogenesisinburkholderiaglumae AT jinwookim quorumsensingcontrolsflagellarmorphogenesisinburkholderiaglumae AT ingyuhwang quorumsensingcontrolsflagellarmorphogenesisinburkholderiaglumae
_version_	1718421540455841792

Quorum sensing controls flagellar morphogenesis in Burkholderia glumae.

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