Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.

We have previously shown that double deletion of the genes for Rac1 and Rac3 GTPases during neuronal development affects late developmental events that perturb the circuitry of the hippocampus, with ensuing epileptic phenotype. These effects include a defect in mossy cells, the major class of excita...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Roberta Pennucci, Stefania Tavano, Diletta Tonoli, Sara Gualdoni, Ivan de Curtis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe61736e8c1c4af3b5580cb005905bda
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe61736e8c1c4af3b5580cb005905bda
record_format	dspace
spelling	oai:doaj.org-article:fe61736e8c1c4af3b5580cb005905bda2021-11-04T06:08:13ZRac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.1932-620310.1371/journal.pone.0024819https://doaj.org/article/fe61736e8c1c4af3b5580cb005905bda2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949760/?tool=EBIhttps://doaj.org/toc/1932-6203We have previously shown that double deletion of the genes for Rac1 and Rac3 GTPases during neuronal development affects late developmental events that perturb the circuitry of the hippocampus, with ensuing epileptic phenotype. These effects include a defect in mossy cells, the major class of excitatory neurons of the hilus. Here, we have addressed the mechanisms that affect the loss of hilar mossy cells in the dorsal hippocampus of mice depleted of the two Rac GTPases. Quantification showed that the loss of mossy cells was evident already at postnatal day 8, soon after these cells become identifiable by a specific marker in the dorsal hilus. Comparative analysis of the hilar region from control and double mutant mice revealed that synaptogenesis was affected in the double mutants, with strongly reduced presynaptic input from dentate granule cells. We found that apoptosis was equally low in the hippocampus of both control and double knockout mice. Labelling with bromodeoxyuridine at embryonic day 12.5 showed no evident difference in the proliferation of neuronal precursors in the hippocampal primordium, while differences in the number of bromodeoxyuridine-labelled cells in the developing hilus revealed a defect in the migration of immature, developing mossy cells in the brain of double knockout mice. Overall, our data show that Rac1 and Rac3 GTPases participate in the normal development of hilar mossy cells, and indicate that they are involved in the regulation of the migration of the mossy cell precursor by preventing their arrival to the dorsal hilus.Roberta PennucciStefania TavanoDiletta TonoliSara GualdoniIvan de CurtisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24819 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Roberta Pennucci Stefania Tavano Diletta Tonoli Sara Gualdoni Ivan de Curtis Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
description	We have previously shown that double deletion of the genes for Rac1 and Rac3 GTPases during neuronal development affects late developmental events that perturb the circuitry of the hippocampus, with ensuing epileptic phenotype. These effects include a defect in mossy cells, the major class of excitatory neurons of the hilus. Here, we have addressed the mechanisms that affect the loss of hilar mossy cells in the dorsal hippocampus of mice depleted of the two Rac GTPases. Quantification showed that the loss of mossy cells was evident already at postnatal day 8, soon after these cells become identifiable by a specific marker in the dorsal hilus. Comparative analysis of the hilar region from control and double mutant mice revealed that synaptogenesis was affected in the double mutants, with strongly reduced presynaptic input from dentate granule cells. We found that apoptosis was equally low in the hippocampus of both control and double knockout mice. Labelling with bromodeoxyuridine at embryonic day 12.5 showed no evident difference in the proliferation of neuronal precursors in the hippocampal primordium, while differences in the number of bromodeoxyuridine-labelled cells in the developing hilus revealed a defect in the migration of immature, developing mossy cells in the brain of double knockout mice. Overall, our data show that Rac1 and Rac3 GTPases participate in the normal development of hilar mossy cells, and indicate that they are involved in the regulation of the migration of the mossy cell precursor by preventing their arrival to the dorsal hilus.
format	article
author	Roberta Pennucci Stefania Tavano Diletta Tonoli Sara Gualdoni Ivan de Curtis
author_facet	Roberta Pennucci Stefania Tavano Diletta Tonoli Sara Gualdoni Ivan de Curtis
author_sort	Roberta Pennucci
title	Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
title_short	Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
title_full	Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
title_fullStr	Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
title_full_unstemmed	Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
title_sort	rac1 and rac3 gtpases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fe61736e8c1c4af3b5580cb005905bda
work_keys_str_mv	AT robertapennucci rac1andrac3gtpasesregulatethedevelopmentofhilarmossycellsbyaffectingthemigrationoftheirprecursorstothehilus AT stefaniatavano rac1andrac3gtpasesregulatethedevelopmentofhilarmossycellsbyaffectingthemigrationoftheirprecursorstothehilus AT dilettatonoli rac1andrac3gtpasesregulatethedevelopmentofhilarmossycellsbyaffectingthemigrationoftheirprecursorstothehilus AT saragualdoni rac1andrac3gtpasesregulatethedevelopmentofhilarmossycellsbyaffectingthemigrationoftheirprecursorstothehilus AT ivandecurtis rac1andrac3gtpasesregulatethedevelopmentofhilarmossycellsbyaffectingthemigrationoftheirprecursorstothehilus
_version_	1718445189900533760

Rac1 and Rac3 GTPases regulate the development of hilar mossy cells by affecting the migration of their precursors to the hilus.

Ejemplares similares