Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients

Abstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase...

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Autores principales:	Atsushi Yamanaka, Hisham Ahmed Imad, Weerapong Phumratanaprapin, Juthamas Phadungsombat, Eiji Konishi, Tatsuo Shioda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe6708c7f6214a3189d92335ffda592b
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spelling	oai:doaj.org-article:fe6708c7f6214a3189d92335ffda592b2021-12-02T17:38:26ZAntibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients10.1038/s41598-021-91793-02045-2322https://doaj.org/article/fe6708c7f6214a3189d92335ffda592b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91793-0https://doaj.org/toc/2045-2322Abstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection.Atsushi YamanakaHisham Ahmed ImadWeerapong PhumratanaprapinJuthamas PhadungsombatEiji KonishiTatsuo ShiodaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
description	Abstract Dengue virus (DENV) causes dengue fever (DF) and dengue hemorrhagic fever in humans. Some DF patients suddenly develop severe symptoms around the defervescent period. Although the pathogenic mechanism of the severe symptoms has not been fully elucidated, the viremia level in the early phase has been shown to correlate with the disease severity. One of the hypotheses is that a phenomenon called antibody-dependent enhancement (ADE) of infection leads to high level of viremia. To examine the plausibility of this hypothesis, we examined the relationship between in vitro ADE activity and in vivo viral load quantity in six patients with dengue diseases. Blood samples were collected at multiple time points between the acute and defervescent phases, and the balance between neutralizing and enhancing activities against the autologous and prototype viruses was examined. As the antibody levels against DENV were rapidly increased, ADE activity was decreased over time or partially maintained against some viruses at low serum dilution. In addition, positive correlations were observed between ADE activity representing in vitro progeny virus production and viremia levels in patient plasma samples. The measurement of ADE activity in dengue-seropositive samples may help to predict the level of viral load in the subsequent DENV infection.
format	article
author	Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda
author_facet	Atsushi Yamanaka Hisham Ahmed Imad Weerapong Phumratanaprapin Juthamas Phadungsombat Eiji Konishi Tatsuo Shioda
author_sort	Atsushi Yamanaka
title	Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
title_short	Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
title_full	Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
title_fullStr	Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
title_full_unstemmed	Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
title_sort	antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fe6708c7f6214a3189d92335ffda592b
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Antibody-dependent enhancement representing in vitro infective progeny virus titer correlates with the viremia level in dengue patients

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