DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.

Haloferax volcanii uses extracellular DNA as a source for carbon, nitrogen, and phosphorous. However, it can also grow to a limited extend in the absence of added phosphorous, indicating that it contains an intracellular phosphate storage molecule. As Hfx. volcanii is polyploid, it was investigated...

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Autores principales:	Karolin Zerulla, Scott Chimileski, Daniela Näther, Uri Gophna, R Thane Papke, Jörg Soppa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe69b24fba0740e981af227ec6416a33
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spelling	oai:doaj.org-article:fe69b24fba0740e981af227ec6416a332021-11-18T08:23:20ZDNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.1932-620310.1371/journal.pone.0094819https://doaj.org/article/fe69b24fba0740e981af227ec6416a332014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24733558/?tool=EBIhttps://doaj.org/toc/1932-6203Haloferax volcanii uses extracellular DNA as a source for carbon, nitrogen, and phosphorous. However, it can also grow to a limited extend in the absence of added phosphorous, indicating that it contains an intracellular phosphate storage molecule. As Hfx. volcanii is polyploid, it was investigated whether DNA might be used as storage polymer, in addition to its role as genetic material. It could be verified that during phosphate starvation cells multiply by distributing as well as by degrading their chromosomes. In contrast, the number of ribosomes stayed constant, revealing that ribosomes are distributed to descendant cells, but not degraded. These results suggest that the phosphate of phosphate-containing biomolecules (other than DNA and RNA) originates from that stored in DNA, not in rRNA. Adding phosphate to chromosome depleted cells rapidly restores polyploidy. Quantification of desiccation survival of cells with different ploidy levels showed that under phosphate starvation Hfx. volcanii diminishes genetic advantages of polyploidy in favor of cell multiplication. The consequences of the usage of genomic DNA as phosphate storage polymer are discussed as well as the hypothesis that DNA might have initially evolved in evolution as a storage polymer, and the various genetic benefits evolved later.Karolin ZerullaScott ChimileskiDaniela NätherUri GophnaR Thane PapkeJörg SoppaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94819 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karolin Zerulla Scott Chimileski Daniela Näther Uri Gophna R Thane Papke Jörg Soppa DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
description	Haloferax volcanii uses extracellular DNA as a source for carbon, nitrogen, and phosphorous. However, it can also grow to a limited extend in the absence of added phosphorous, indicating that it contains an intracellular phosphate storage molecule. As Hfx. volcanii is polyploid, it was investigated whether DNA might be used as storage polymer, in addition to its role as genetic material. It could be verified that during phosphate starvation cells multiply by distributing as well as by degrading their chromosomes. In contrast, the number of ribosomes stayed constant, revealing that ribosomes are distributed to descendant cells, but not degraded. These results suggest that the phosphate of phosphate-containing biomolecules (other than DNA and RNA) originates from that stored in DNA, not in rRNA. Adding phosphate to chromosome depleted cells rapidly restores polyploidy. Quantification of desiccation survival of cells with different ploidy levels showed that under phosphate starvation Hfx. volcanii diminishes genetic advantages of polyploidy in favor of cell multiplication. The consequences of the usage of genomic DNA as phosphate storage polymer are discussed as well as the hypothesis that DNA might have initially evolved in evolution as a storage polymer, and the various genetic benefits evolved later.
format	article
author	Karolin Zerulla Scott Chimileski Daniela Näther Uri Gophna R Thane Papke Jörg Soppa
author_facet	Karolin Zerulla Scott Chimileski Daniela Näther Uri Gophna R Thane Papke Jörg Soppa
author_sort	Karolin Zerulla
title	DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
title_short	DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
title_full	DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
title_fullStr	DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
title_full_unstemmed	DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
title_sort	dna as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fe69b24fba0740e981af227ec6416a33
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DNA as a phosphate storage polymer and the alternative advantages of polyploidy for growth or survival.

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