Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells

Ayşegül Tura,1 Aizhan Alt,1 Julia Lüke,1 Salvatore Grisanti,1 Christos Haritoglou,2 Carsten H Meyer,3 Khaled Nassar,1 Matthias Lüke1 On behalf of the International Chromovitrectomy Collaboration 1Department of Ophthalmology, University of Schleswig-Holstein, Lüb...

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Autores principales:	Tura A, Alt A, Lüke J, Grisanti S, Haritoglou C, Meyer CH, Nassar K, Lüke M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Acid Violet-17 retinal pigment epithelial cells biocompatibility viability differentiation Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/fe6a4889acd8461bb30258494c349099
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spelling	oai:doaj.org-article:fe6a4889acd8461bb30258494c3490992021-12-02T08:39:14ZBiocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells1177-5483https://doaj.org/article/fe6a4889acd8461bb30258494c3490992016-07-01T00:00:00Zhttps://www.dovepress.com/biocompatibility-of-the-vital-dye-acid-violet-17-on-retinal-pigment-ep-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Ayşegül Tura,1 Aizhan Alt,1 Julia Lüke,1 Salvatore Grisanti,1 Christos Haritoglou,2 Carsten H Meyer,3 Khaled Nassar,1 Matthias Lüke1 On behalf of the International Chromovitrectomy Collaboration 1Department of Ophthalmology, University of Schleswig-Holstein, Lübeck, Germany; 2Department of Ophthalmology, Ludwig-Maximilians University, Munich, Germany; 3Department of Ophthalmology, Pallas Clinic, Aarau, Switzerland Purpose: To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17). Methods: Bovine RPE cells were incubated with AV-17 (0.0625–0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19. Results: Exposure to AV-17 at the concentrations of 0.25–0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19. Conclusion: AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure. Keywords: Acid Violet-17, retinal pigment epithelial cells, biocompatibility, viability, differentiationTura AAlt ALüke JGrisanti SHaritoglou CMeyer CHNassar KLüke MDove Medical PressarticleAcid Violet-17retinal pigment epithelial cellsbiocompatibilityviabilitydifferentiationOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2016, Iss Issue 1, Pp 1435-1445 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acid Violet-17 retinal pigment epithelial cells biocompatibility viability differentiation Ophthalmology RE1-994
spellingShingle	Acid Violet-17 retinal pigment epithelial cells biocompatibility viability differentiation Ophthalmology RE1-994 Tura A Alt A Lüke J Grisanti S Haritoglou C Meyer CH Nassar K Lüke M Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
description	Ayşegül Tura,1 Aizhan Alt,1 Julia Lüke,1 Salvatore Grisanti,1 Christos Haritoglou,2 Carsten H Meyer,3 Khaled Nassar,1 Matthias Lüke1 On behalf of the International Chromovitrectomy Collaboration 1Department of Ophthalmology, University of Schleswig-Holstein, Lübeck, Germany; 2Department of Ophthalmology, Ludwig-Maximilians University, Munich, Germany; 3Department of Ophthalmology, Pallas Clinic, Aarau, Switzerland Purpose: To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17). Methods: Bovine RPE cells were incubated with AV-17 (0.0625–0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19. Results: Exposure to AV-17 at the concentrations of 0.25–0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19. Conclusion: AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure. Keywords: Acid Violet-17, retinal pigment epithelial cells, biocompatibility, viability, differentiation
format	article
author	Tura A Alt A Lüke J Grisanti S Haritoglou C Meyer CH Nassar K Lüke M
author_facet	Tura A Alt A Lüke J Grisanti S Haritoglou C Meyer CH Nassar K Lüke M
author_sort	Tura A
title	Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
title_short	Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
title_full	Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
title_fullStr	Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
title_full_unstemmed	Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
title_sort	biocompatibility of the vital dye acid violet-17 on retinal pigment epithelial cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/fe6a4889acd8461bb30258494c349099
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Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells

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