Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma

Abstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression...

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Autores principales:	Qing Yuan, Song‐Quan Wang, Guang‐Tao Zhang, Jie He, Zhi‐Dan Liu, Ming‐Rong Wang, Hong‐Qing Cai, Jing‐Hai Wan
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	glioma immune response prognosis SERPINA3 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fe6d5b9851a54c37a27416b077301937
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spelling	oai:doaj.org-article:fe6d5b9851a54c37a27416b0773019372021-11-12T19:57:15ZHighly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma2050-452710.1002/iid3.515https://doaj.org/article/fe6d5b9851a54c37a27416b0773019372021-12-01T00:00:00Zhttps://doi.org/10.1002/iid3.515https://doaj.org/toc/2050-4527Abstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression in the biological process, prognosis, and immune significance in glioma. Methods We analyzed the biological functions of SERPINA3 through data from the Chinese Glioma Genome Atlas databases. Differentially expressed genes and enrichment analysis were performed and correlations between SERPINA3 expression and immune cell infiltration were analyzed. Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. The correlations between the expression and clinical‐pathological parameters as well as other biomarkers were examined. Results Univariate and multivariate regression both indicated that the level of SERPINA3 transcript represented an independent prognostic factor. High levels of SERPINA3 correlated with poor survival in patients with glioma. Expression of SERPINA3 mRNA was observed positively correlated with MCM6, IGFBP2, and FKBP10. Enrichment analysis showed SERPINA3 mainly enriched in immune‐related terms and signaling pathways including MAPK, TNF, P53, PI3K‐Akt, nuclear factor‐κB. Immune infiltration analysis further declare the SERPINA3 expression negatively correlated with levels of Macrophages M1, native CD4+ T cell, monocytes, and Mast cell activated. And overexpression of SERPINA3 correlated with low CD4+ T cell infiltration in glioma tissues. Conclusions SERPINA3 may play a key role in the biological process of glioma cells especially in immune suppression activities. SERPINA3 may serve as an independent survival prediction factor in glioma patients.Qing YuanSong‐Quan WangGuang‐Tao ZhangJie HeZhi‐Dan LiuMing‐Rong WangHong‐Qing CaiJing‐Hai WanWileyarticlegliomaimmune responseprognosisSERPINA3Immunologic diseases. AllergyRC581-607ENImmunity, Inflammation and Disease, Vol 9, Iss 4, Pp 1618-1630 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma immune response prognosis SERPINA3 Immunologic diseases. Allergy RC581-607
spellingShingle	glioma immune response prognosis SERPINA3 Immunologic diseases. Allergy RC581-607 Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
description	Abstract Introduction The prognosis of patients with glioma is dismal. It has been reported that Serpin peptidase inhibitor clade A member 3 (SERPINA3) is associated with the mobility and invasion of tumor cells. Our study was designed to explore the value of SERPINA3 messenger RNA (mRNA) expression in the biological process, prognosis, and immune significance in glioma. Methods We analyzed the biological functions of SERPINA3 through data from the Chinese Glioma Genome Atlas databases. Differentially expressed genes and enrichment analysis were performed and correlations between SERPINA3 expression and immune cell infiltration were analyzed. Further, we validated the expression and the survival prediction role of SERPINA3 by using tissue microarrays and RNAscope in situ hybridization in 321 gliomas. The correlations between the expression and clinical‐pathological parameters as well as other biomarkers were examined. Results Univariate and multivariate regression both indicated that the level of SERPINA3 transcript represented an independent prognostic factor. High levels of SERPINA3 correlated with poor survival in patients with glioma. Expression of SERPINA3 mRNA was observed positively correlated with MCM6, IGFBP2, and FKBP10. Enrichment analysis showed SERPINA3 mainly enriched in immune‐related terms and signaling pathways including MAPK, TNF, P53, PI3K‐Akt, nuclear factor‐κB. Immune infiltration analysis further declare the SERPINA3 expression negatively correlated with levels of Macrophages M1, native CD4+ T cell, monocytes, and Mast cell activated. And overexpression of SERPINA3 correlated with low CD4+ T cell infiltration in glioma tissues. Conclusions SERPINA3 may play a key role in the biological process of glioma cells especially in immune suppression activities. SERPINA3 may serve as an independent survival prediction factor in glioma patients.
format	article
author	Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan
author_facet	Qing Yuan Song‐Quan Wang Guang‐Tao Zhang Jie He Zhi‐Dan Liu Ming‐Rong Wang Hong‐Qing Cai Jing‐Hai Wan
author_sort	Qing Yuan
title	Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
title_short	Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
title_full	Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
title_fullStr	Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
title_full_unstemmed	Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma
title_sort	highly expressed of serpina3 indicated poor prognosis and involved in immune suppression in glioma
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/fe6d5b9851a54c37a27416b077301937
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_version_	1718430352028991488

Highly expressed of SERPINA3 indicated poor prognosis and involved in immune suppression in glioma

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