Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India

INTRODUCTION: Focused studies in different geographic regions would delineate the underlying biological differences and molecular alterations in non-small cell lung cancer (NSCLC) worldwide. Previous studies in literature have documented limited characterization by studying a minimal number of biolo...

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Autores principales:	Shalinee Rao, Michael Leonard Anthony, Nilotpal Chowdhury, Rajesh Kathrotia, Mayank Mishra, Manisha Naithani, Girish Sindhwani, Neha Singh
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	characterization immunoexpression molecular profile non-small cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/fe743491a1074ef59c1246ef8252401f
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spelling	oai:doaj.org-article:fe743491a1074ef59c1246ef8252401f2021-11-12T10:17:19ZMolecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India1477-316310.4103/jcar.jcar_14_21https://doaj.org/article/fe743491a1074ef59c1246ef8252401f2021-01-01T00:00:00Zhttp://www.carcinogenesis.com/article.asp?issn=1477-3163;year=2021;volume=20;issue=1;spage=17;epage=17;aulast=Raohttps://doaj.org/toc/1477-3163INTRODUCTION: Focused studies in different geographic regions would delineate the underlying biological differences and molecular alterations in non-small cell lung cancer (NSCLC) worldwide. Previous studies in literature have documented limited characterization by studying a minimal number of biological markers. This study was done to evaluate expression of multiple immunomarkers including diagnostic, prognostic, and predictive markers in NSCLC for its characterization.MATERIALS AND METHODS: This was an observational study conducted on 60 consecutive cases of NSCLC. Immunomarkers comprising of p63, p40, TTF-1, napsin A, B-Raf, c-Met, phospho-AKT (P-AKT), PTEN, anaplastic lymphoma kinase (ALK), epidermal growth factor receptor (EGFR) and K-Ras, synaptophysin, chromogranin and pan-cytokeratin were evaluated on paraffin-embedded tissue sections of NSCLC.RESULTS: Age of patients with NSCLC in our study ranged from 35 to 90 years, and 93.3% of them were chronic smokers. 93.3% of cases presented in late stages (Stages III and IV) and 78% of cases were squamous cell carcinoma (SCC). EGFR positivity was noted in 83.3% of cases. ALK was positive in one case while C-Met and PTEN immunopositivity was noted in only two cases. Ten cases showed positivity for K-Ras and 90% of these were SCC. Ten cases were positive for B-Raf and 80% of these were SCC. 30% of cases showed immunopositivity for P-AKT. None of the molecular markers was found to have statistically significant correlation with clinicopathological parameters.CONCLUSION: SCC is the predominant histological subtype of NSCLC in the region of Uttarakhand, India, with a high proportion of cases harboring EGFR mutation. Variable expression of K-Ras, P-AKT, ALK 1, and PTEN in NSCLC signifies that molecular profile of every case is individualistic and independent. We attribute this to ethnicity, influence of implicated substance or metabolite in tobacco, and variable mutations incurred in tumor cells over a period of time.Shalinee RaoMichael Leonard AnthonyNilotpal ChowdhuryRajesh KathrotiaMayank MishraManisha NaithaniGirish SindhwaniNeha SinghWolters Kluwer Medknow Publicationsarticlecharacterizationimmunoexpressionmolecular profilenon-small cell lung cancerNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENJournal of Carcinogenesis, Vol 20, Iss 1, Pp 17-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	characterization immunoexpression molecular profile non-small cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	characterization immunoexpression molecular profile non-small cell lung cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Shalinee Rao Michael Leonard Anthony Nilotpal Chowdhury Rajesh Kathrotia Mayank Mishra Manisha Naithani Girish Sindhwani Neha Singh Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
description	INTRODUCTION: Focused studies in different geographic regions would delineate the underlying biological differences and molecular alterations in non-small cell lung cancer (NSCLC) worldwide. Previous studies in literature have documented limited characterization by studying a minimal number of biological markers. This study was done to evaluate expression of multiple immunomarkers including diagnostic, prognostic, and predictive markers in NSCLC for its characterization.MATERIALS AND METHODS: This was an observational study conducted on 60 consecutive cases of NSCLC. Immunomarkers comprising of p63, p40, TTF-1, napsin A, B-Raf, c-Met, phospho-AKT (P-AKT), PTEN, anaplastic lymphoma kinase (ALK), epidermal growth factor receptor (EGFR) and K-Ras, synaptophysin, chromogranin and pan-cytokeratin were evaluated on paraffin-embedded tissue sections of NSCLC.RESULTS: Age of patients with NSCLC in our study ranged from 35 to 90 years, and 93.3% of them were chronic smokers. 93.3% of cases presented in late stages (Stages III and IV) and 78% of cases were squamous cell carcinoma (SCC). EGFR positivity was noted in 83.3% of cases. ALK was positive in one case while C-Met and PTEN immunopositivity was noted in only two cases. Ten cases showed positivity for K-Ras and 90% of these were SCC. Ten cases were positive for B-Raf and 80% of these were SCC. 30% of cases showed immunopositivity for P-AKT. None of the molecular markers was found to have statistically significant correlation with clinicopathological parameters.CONCLUSION: SCC is the predominant histological subtype of NSCLC in the region of Uttarakhand, India, with a high proportion of cases harboring EGFR mutation. Variable expression of K-Ras, P-AKT, ALK 1, and PTEN in NSCLC signifies that molecular profile of every case is individualistic and independent. We attribute this to ethnicity, influence of implicated substance or metabolite in tobacco, and variable mutations incurred in tumor cells over a period of time.
format	article
author	Shalinee Rao Michael Leonard Anthony Nilotpal Chowdhury Rajesh Kathrotia Mayank Mishra Manisha Naithani Girish Sindhwani Neha Singh
author_facet	Shalinee Rao Michael Leonard Anthony Nilotpal Chowdhury Rajesh Kathrotia Mayank Mishra Manisha Naithani Girish Sindhwani Neha Singh
author_sort	Shalinee Rao
title	Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
title_short	Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
title_full	Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
title_fullStr	Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
title_full_unstemmed	Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India
title_sort	molecular characterization of lung carcinomas: a study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in uttarakhand, india
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/fe743491a1074ef59c1246ef8252401f
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Molecular characterization of lung carcinomas: A study on diagnostic, predictive, and prognostic markers using immunohistochemical analysis at a tertiary care center in Uttarakhand, India

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