Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation

Abstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the...

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Autores principales:	Qunhao Zheng, Zhiping Wang, Mengyan Zhang, Yilin Yu, Rui Chen, Tianzhu Lu, Lingyun Liu, Jiayu Ma, Tianxiu Liu, Hongying Zheng, Hui Li, Jiancheng Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	SEC61G Lung adenocarcinoma Novel biomarkers Prognosis Proliferation Invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/fe7a6cf687e24966832fd6bf854892c5
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spelling	oai:doaj.org-article:fe7a6cf687e24966832fd6bf854892c52021-11-14T12:29:47ZPrognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation10.1186/s12885-021-08957-41471-2407https://doaj.org/article/fe7a6cf687e24966832fd6bf854892c52021-11-01T00:00:00Zhttps://doi.org/10.1186/s12885-021-08957-4https://doaj.org/toc/1471-2407Abstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the prognostic value and potential biological function of the SEC61G gene in LUAD. Methods Public datasets were used for SEC61G expression analyses. The prognostic value of SEC61G in LUAD was investigated using the Kaplan–Meier survival and Cox analyses. The correlation between the methylation level of SEC61G and its mRNA expression was evaluated via cBioPortal. Additionally, MethSurv was used to determine the prognostic value of the SEC61G methylation levels in LUAD. Functional enrichment analysis was conducted to explore the potential mechanism of SEC61G. Also, single sample GSEA (ssGSEA) and TIMER online tool were applied to identify the correlation between SEC61G and immune filtration. Furthermore, cell functional experiments were conducted to verify the biological behavior of SEC61G in lung adenocarcinoma cells (LAC). Results SEC61G was upregulated in pan-cancers, including LUAD. High SEC61G expression was significantly correlated with worse prognosis in LUAD patients. Multivariate analysis demonstrated that high SEC61G expression was an independent prognostic factor in the TCGA cohort. (HR = 1.760 95% CI: 1.297–2.388, p < 0.001). The methylation level of SEC61G negatively correlated with the SEC61G expression (R = − 0.290, p < 0.001), and patients with low SEC61G methylation had worse overall survival. (p = 0.0014). Proliferation-associated terms such as cell cycle and cell division were significantly enriched in GO and KEGG analysis. Vitro experiments demonstrated that knockdown of SEC61G resulted in decreased cell proliferation, invasion and facilitated apoptosis in LAC. GSEA analysis found that SEC61G expression was associated with the E2F targets. Moreover, SEC61G expression was negatively correlated with the immune cell infiltration including CD4+ T cell, CD8+ T cell, B cell, macrophage, neutrophil, and dendritic cell. Conclusion Our study indicated that overexpression of SEC61G was significantly associated with poor prognosis of LUAD patients and the malignant phenotypes of LUAD cells, suggesting that it could be a novel prognostic biomarker and potential therapeutic target of LUAD.Qunhao ZhengZhiping WangMengyan ZhangYilin YuRui ChenTianzhu LuLingyun LiuJiayu MaTianxiu LiuHongying ZhengHui LiJiancheng LiBMCarticleSEC61GLung adenocarcinomaNovel biomarkersPrognosisProliferationInvasionNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBMC Cancer, Vol 21, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SEC61G Lung adenocarcinoma Novel biomarkers Prognosis Proliferation Invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	SEC61G Lung adenocarcinoma Novel biomarkers Prognosis Proliferation Invasion Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
description	Abstract Background Studies have shown that the Sec61 gamma subunit (SEC61G) is overexpressed in several tumors and could serve as a potential prognostic marker. However, the correlation between SEC61G and lung adenocarcinoma (LUAD) remains unclear. In the current study, we aimed to demonstrate the prognostic value and potential biological function of the SEC61G gene in LUAD. Methods Public datasets were used for SEC61G expression analyses. The prognostic value of SEC61G in LUAD was investigated using the Kaplan–Meier survival and Cox analyses. The correlation between the methylation level of SEC61G and its mRNA expression was evaluated via cBioPortal. Additionally, MethSurv was used to determine the prognostic value of the SEC61G methylation levels in LUAD. Functional enrichment analysis was conducted to explore the potential mechanism of SEC61G. Also, single sample GSEA (ssGSEA) and TIMER online tool were applied to identify the correlation between SEC61G and immune filtration. Furthermore, cell functional experiments were conducted to verify the biological behavior of SEC61G in lung adenocarcinoma cells (LAC). Results SEC61G was upregulated in pan-cancers, including LUAD. High SEC61G expression was significantly correlated with worse prognosis in LUAD patients. Multivariate analysis demonstrated that high SEC61G expression was an independent prognostic factor in the TCGA cohort. (HR = 1.760 95% CI: 1.297–2.388, p < 0.001). The methylation level of SEC61G negatively correlated with the SEC61G expression (R = − 0.290, p < 0.001), and patients with low SEC61G methylation had worse overall survival. (p = 0.0014). Proliferation-associated terms such as cell cycle and cell division were significantly enriched in GO and KEGG analysis. Vitro experiments demonstrated that knockdown of SEC61G resulted in decreased cell proliferation, invasion and facilitated apoptosis in LAC. GSEA analysis found that SEC61G expression was associated with the E2F targets. Moreover, SEC61G expression was negatively correlated with the immune cell infiltration including CD4+ T cell, CD8+ T cell, B cell, macrophage, neutrophil, and dendritic cell. Conclusion Our study indicated that overexpression of SEC61G was significantly associated with poor prognosis of LUAD patients and the malignant phenotypes of LUAD cells, suggesting that it could be a novel prognostic biomarker and potential therapeutic target of LUAD.
format	article
author	Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li
author_facet	Qunhao Zheng Zhiping Wang Mengyan Zhang Yilin Yu Rui Chen Tianzhu Lu Lingyun Liu Jiayu Ma Tianxiu Liu Hongying Zheng Hui Li Jiancheng Li
author_sort	Qunhao Zheng
title	Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
title_short	Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
title_full	Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
title_fullStr	Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
title_full_unstemmed	Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
title_sort	prognostic value of sec61g in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation
publisher	BMC
publishDate	2021
url	https://doaj.org/article/fe7a6cf687e24966832fd6bf854892c5
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Prognostic value of SEC61G in lung adenocarcinoma: a comprehensive study based on bioinformatics and in vitro validation

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