Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods

Lauren E Cosby,1 Kil Ho Lee,2 Thomas J Knobloch,3 Christopher M Weghorst,3 Jessica O Winter1,2 1Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; 2William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA; 3Col...

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Autores principales:	Cosby LE, Lee KH, Knobloch TJ, Weghorst CM, Winter JO
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	black raspberries lutein oral cancer scalable nanomanufacturing nanoprecipitation emulsification Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fe7b32c8dd1e4d4eb4741d4b5fd1d966
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spelling	oai:doaj.org-article:fe7b32c8dd1e4d4eb4741d4b5fd1d9662021-12-02T15:28:29ZComparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods1178-2013https://doaj.org/article/fe7b32c8dd1e4d4eb4741d4b5fd1d9662020-10-01T00:00:00Zhttps://www.dovepress.com/comparative-encapsulation-efficiency-of-lutein-in-micelles-synthesized-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lauren E Cosby,1 Kil Ho Lee,2 Thomas J Knobloch,3 Christopher M Weghorst,3 Jessica O Winter1,2 1Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; 2William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA; 3College of Public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USACorrespondence: Jessica O Winter Tel +1 614 247 7668Email winter.63@osu.eduPurpose: Black raspberries (BRBs) and their anthocyanin-rich hydrophilic fractions (BRB-H) have exhibited significant chemopreventative activity across aerodigestive cancers. Lutein, the primary component of the BRB lipophilic fraction (BRB-L), also demonstrates bioactivity potential, but is less well characterized, in part because of its poor, innate bioavailability. For these lipophilic compounds to be accurately evaluated for anticancer efficacy, it is necessary to increase their functional bioavailability using delivery vehicles. Lutein has been delivered in commercial settings in emulsion form. However, emulsions are unstable, particularly in the gastrointestinal tract, which limit their use as an oral nutraceutical. Here, we evaluated lutein encapsulation and cellular uptake for nanoparticle (NP) delivery vehicles composed of three different materials synthesized via two different approaches.Methods: Specifically, NPs were synthesized via smaller scale batch interfacial instability (II) sonication and semi-continuous high throughput electrohydrodynamic-mediated mixing nanoprecipitation (EM-NP) methods using polystyrene-polyethylene oxide (PSPEO) or polycaprolactone-polyethylene glycol (PCLPEG) block copolymers and PHOSPHOLIPON 90G® (P90G, Lipoid GmbH) lipids. Size distribution, lutein encapsulation efficiency (EE), and cellular uptake and delivery were evaluated for each NP formulation.Results: NPs produced via high throughput EM-NP had higher EEs than NPs produced via batch II sonication, and P90G had the greatest EE (55%) and elicited faster cellular uptake in premalignant oral epithelial cells (SCC83) compared to other delivery systems.Conclusion: These qualities suggest P90G could be a beneficial candidate for future lutein in vitro delivery research and clinical translation for oral cancer prevention.Keywords: black raspberries, lutein, oral cancer, scalable nanomanufacturing, nanoprecipitation, emulsificationCosby LELee KHKnobloch TJWeghorst CMWinter JODove Medical Pressarticleblack raspberriesluteinoral cancerscalable nanomanufacturingnanoprecipitationemulsificationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 8217-8230 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	black raspberries lutein oral cancer scalable nanomanufacturing nanoprecipitation emulsification Medicine (General) R5-920
spellingShingle	black raspberries lutein oral cancer scalable nanomanufacturing nanoprecipitation emulsification Medicine (General) R5-920 Cosby LE Lee KH Knobloch TJ Weghorst CM Winter JO Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
description	Lauren E Cosby,1 Kil Ho Lee,2 Thomas J Knobloch,3 Christopher M Weghorst,3 Jessica O Winter1,2 1Biomedical Engineering, The Ohio State University, Columbus, OH 43210, USA; 2William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA; 3College of Public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USACorrespondence: Jessica O Winter Tel +1 614 247 7668Email winter.63@osu.eduPurpose: Black raspberries (BRBs) and their anthocyanin-rich hydrophilic fractions (BRB-H) have exhibited significant chemopreventative activity across aerodigestive cancers. Lutein, the primary component of the BRB lipophilic fraction (BRB-L), also demonstrates bioactivity potential, but is less well characterized, in part because of its poor, innate bioavailability. For these lipophilic compounds to be accurately evaluated for anticancer efficacy, it is necessary to increase their functional bioavailability using delivery vehicles. Lutein has been delivered in commercial settings in emulsion form. However, emulsions are unstable, particularly in the gastrointestinal tract, which limit their use as an oral nutraceutical. Here, we evaluated lutein encapsulation and cellular uptake for nanoparticle (NP) delivery vehicles composed of three different materials synthesized via two different approaches.Methods: Specifically, NPs were synthesized via smaller scale batch interfacial instability (II) sonication and semi-continuous high throughput electrohydrodynamic-mediated mixing nanoprecipitation (EM-NP) methods using polystyrene-polyethylene oxide (PSPEO) or polycaprolactone-polyethylene glycol (PCLPEG) block copolymers and PHOSPHOLIPON 90G® (P90G, Lipoid GmbH) lipids. Size distribution, lutein encapsulation efficiency (EE), and cellular uptake and delivery were evaluated for each NP formulation.Results: NPs produced via high throughput EM-NP had higher EEs than NPs produced via batch II sonication, and P90G had the greatest EE (55%) and elicited faster cellular uptake in premalignant oral epithelial cells (SCC83) compared to other delivery systems.Conclusion: These qualities suggest P90G could be a beneficial candidate for future lutein in vitro delivery research and clinical translation for oral cancer prevention.Keywords: black raspberries, lutein, oral cancer, scalable nanomanufacturing, nanoprecipitation, emulsification
format	article
author	Cosby LE Lee KH Knobloch TJ Weghorst CM Winter JO
author_facet	Cosby LE Lee KH Knobloch TJ Weghorst CM Winter JO
author_sort	Cosby LE
title	Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
title_short	Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
title_full	Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
title_fullStr	Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
title_full_unstemmed	Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods
title_sort	comparative encapsulation efficiency of lutein in micelles synthesized via batch and high throughput methods
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/fe7b32c8dd1e4d4eb4741d4b5fd1d966
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Comparative Encapsulation Efficiency of Lutein in Micelles Synthesized via Batch and High Throughput Methods

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