Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish

Abstract Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and fun...

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Autores principales:	Henri A. J. Puttonen, Svetlana Semenova, Maria Sundvik, Pertti Panula
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe8099b627f841cb8eda289a31e02d02
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spelling	oai:doaj.org-article:fe8099b627f841cb8eda289a31e02d022021-12-02T11:52:17ZStorage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish10.1038/s41598-017-02981-w2045-2322https://doaj.org/article/fe8099b627f841cb8eda289a31e02d022017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02981-whttps://doaj.org/toc/2045-2322Abstract Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and function of the histaminergic system in larval zebrafish following inhibition of VMAT2 function by reserpine. We found that reserpine treatment greatly reduced histamine immunoreactivity in neurons and an almost total disappearance of histamine-containing nerve fibers in the dorsal telencephalon and habenula, the most densely innervated targets of the hypothalamic histamine neurons. The reserpine treated larvae had an impaired histamine-dependent dark-induced flash response seen during the first second after onset of darkness, implying that function of the histaminergic network is VMAT2 dependent. Levels of histamine and other monoamines were decreased in reserpine treated animals. This study provides conclusive evidence of the relevance of VMAT2 in histaminergic neurotransmission, further implying that the storage and release mechanism of neural histamine is comparable to that of other monoamines. Our results also reveal potential new insights about the roles of monoaminergic neurotransmitters in the regulation of locomotion increase during adaptation to darkness.Henri A. J. PuttonenSvetlana SemenovaMaria SundvikPertti PanulaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Henri A. J. Puttonen Svetlana Semenova Maria Sundvik Pertti Panula Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
description	Abstract Monoaminergic neurotransmission is greatly dependent on the function of the vesicular monoamine transporter VMAT2, which is responsible for loading monoamines into secretory vesicles. The role of VMAT2 in histaminergic neurotransmission is poorly understood. We studied the structure and function of the histaminergic system in larval zebrafish following inhibition of VMAT2 function by reserpine. We found that reserpine treatment greatly reduced histamine immunoreactivity in neurons and an almost total disappearance of histamine-containing nerve fibers in the dorsal telencephalon and habenula, the most densely innervated targets of the hypothalamic histamine neurons. The reserpine treated larvae had an impaired histamine-dependent dark-induced flash response seen during the first second after onset of darkness, implying that function of the histaminergic network is VMAT2 dependent. Levels of histamine and other monoamines were decreased in reserpine treated animals. This study provides conclusive evidence of the relevance of VMAT2 in histaminergic neurotransmission, further implying that the storage and release mechanism of neural histamine is comparable to that of other monoamines. Our results also reveal potential new insights about the roles of monoaminergic neurotransmitters in the regulation of locomotion increase during adaptation to darkness.
format	article
author	Henri A. J. Puttonen Svetlana Semenova Maria Sundvik Pertti Panula
author_facet	Henri A. J. Puttonen Svetlana Semenova Maria Sundvik Pertti Panula
author_sort	Henri A. J. Puttonen
title	Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
title_short	Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
title_full	Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
title_fullStr	Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
title_full_unstemmed	Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish
title_sort	storage of neural histamine and histaminergic neurotransmission is vmat2 dependent in the zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fe8099b627f841cb8eda289a31e02d02
work_keys_str_mv	AT henriajputtonen storageofneuralhistamineandhistaminergicneurotransmissionisvmat2dependentinthezebrafish AT svetlanasemenova storageofneuralhistamineandhistaminergicneurotransmissionisvmat2dependentinthezebrafish AT mariasundvik storageofneuralhistamineandhistaminergicneurotransmissionisvmat2dependentinthezebrafish AT perttipanula storageofneuralhistamineandhistaminergicneurotransmissionisvmat2dependentinthezebrafish
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Storage of neural histamine and histaminergic neurotransmission is VMAT2 dependent in the zebrafish

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