Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo

Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessi...

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Autores principales:	Xianqing Lv, Guangxu Yang, Zhenggang Gong, Xin Cheng, Li Shuai, Liulian Huang, Lihui Chen, Xiaolin Luo, Jing Liu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lignin non-productive adsorption enzymatic hydrolysis poly(N-vinylcaprolactam) phenylsulfonic acid Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/fe8c3d72667d4be0bc720a11c3317adc
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spelling	oai:doaj.org-article:fe8c3d72667d4be0bc720a11c3317adc2021-12-01T14:58:32ZUsing poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo2296-418510.3389/fbioe.2021.804456https://doaj.org/article/fe8c3d72667d4be0bc720a11c3317adc2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.804456/fullhttps://doaj.org/toc/2296-4185Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery.Xianqing LvGuangxu YangZhenggang GongXin ChengLi ShuaiLiulian HuangLihui ChenXiaolin LuoXiaolin LuoJing LiuFrontiers Media S.A.articleligninnon-productive adsorptionenzymatic hydrolysispoly(N-vinylcaprolactam)phenylsulfonic acidBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lignin non-productive adsorption enzymatic hydrolysis poly(N-vinylcaprolactam) phenylsulfonic acid Biotechnology TP248.13-248.65
spellingShingle	lignin non-productive adsorption enzymatic hydrolysis poly(N-vinylcaprolactam) phenylsulfonic acid Biotechnology TP248.13-248.65 Xianqing Lv Guangxu Yang Zhenggang Gong Xin Cheng Li Shuai Liulian Huang Lihui Chen Xiaolin Luo Xiaolin Luo Jing Liu Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
description	Chemical pretreatment followed by enzymatic hydrolysis has been regarded as a viable way to produce fermentable sugars. Phenylsulfonic acid (PSA) pretreatment could efficiently fractionate the non-cellulosic components (hemicelluloses and lignin) from bamboo and result in increased cellulose accessibility that was 10 times that of untreated bamboo. However, deposited lignin could trigger non-productive adsorption to enzymes, which therefore significantly decreased the enzymatic hydrolysis efficiency of PSA-pretreated bamboo substrates. Herein, poly(N-vinylcaprolactam) (PNVCL), a non-ionic surfactant, was developed as a novel additive for overcoming the non-productive adsorption of lignin during enzymatic hydrolysis. PNVCL was found to be not only more effective than those of commonly used lignosulfonate and polyvinyl alcohol for overcoming the negative effect of lignin, but also comparable to the robust Tween 20 and bovine serum albumin additives. A PNVCL loading at 1.2 g/L during enzymatic hydrolysis of PSA pretreated bamboo substrate could achieve an 80% cellulosic enzymatic conversion and meanwhile reduce the cellulase loading by three times as compared to that without additive. Mechanistic investigations indicated that PNVCL could block lignin residues through hydrophobic interactions and the resultant PNVCL coating resisted the adsorption of cellulase via electrostatic repulsion and/or hydration. This practical method can improve the lignocellulosic enzymatic hydrolysis efficiency and thereby increase the productivity and profitability of biorefinery.
format	article
author	Xianqing Lv Guangxu Yang Zhenggang Gong Xin Cheng Li Shuai Liulian Huang Lihui Chen Xiaolin Luo Xiaolin Luo Jing Liu
author_facet	Xianqing Lv Guangxu Yang Zhenggang Gong Xin Cheng Li Shuai Liulian Huang Lihui Chen Xiaolin Luo Xiaolin Luo Jing Liu
author_sort	Xianqing Lv
title	Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
title_short	Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
title_full	Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
title_fullStr	Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
title_full_unstemmed	Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo
title_sort	using poly(n-vinylcaprolactam) to improve the enzymatic hydrolysis efficiency of phenylsulfonic acid-pretreated bamboo
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fe8c3d72667d4be0bc720a11c3317adc
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Using poly(N-Vinylcaprolactam) to Improve the Enzymatic Hydrolysis Efficiency of Phenylsulfonic Acid-Pretreated Bamboo

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