The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells

Kelly Hallman,* Katie Aleck,* Meghan Quigley, Brigitte Dwyer, Victoria Lloyd, Monica Szmyd, Sumi Dinda Biomedical Diagnostic and Therapeutic Sciences, School of Health Sciences, Center for Biomedical Research, Oakland University, Rochester, MI, USA *These...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hallman K, Aleck K, Quigley M, Dwyer B, Lloyd V, Szmyd M, Dinda S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	phytoestrogen ER PR T-47D anti-estrogens Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/fe8db0e36a0f4dc0a73130cf806a57bf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe8db0e36a0f4dc0a73130cf806a57bf
record_format	dspace
spelling	oai:doaj.org-article:fe8db0e36a0f4dc0a73130cf806a57bf2021-12-02T00:16:21ZThe regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells1179-1314https://doaj.org/article/fe8db0e36a0f4dc0a73130cf806a57bf2017-05-01T00:00:00Zhttps://www.dovepress.com/the-regulation-of-steroid-receptors-by-epigallocatechin-3-gallate-in-b-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Kelly Hallman,* Katie Aleck,* Meghan Quigley, Brigitte Dwyer, Victoria Lloyd, Monica Szmyd, Sumi Dinda Biomedical Diagnostic and Therapeutic Sciences, School of Health Sciences, Center for Biomedical Research, Oakland University, Rochester, MI, USA *These authors contributed equally to this work Abstract: It has been reported that phytoestrogen epigallocatechin gallate (EGCG) suppresses cancer cell proliferation and may have antitumor properties. In this study, we analyzed the effects of EGCG on estrogen receptor α (ERα) and progesterone receptor in hormone-dependent T-47D breast cancer cells. Western blot analysis revealed EGCG induced a concentration-dependent decrease in ERα protein levels, with a 56% reduction occurring with 60 µM EGCG when compared to controls. Downregulation of ERα protein levels was observed after 24-hour co-treatment of T-47D cells with 60 µM EGCG and 10 nM 17β-estradiol (E2). The proliferative effect of E2 on cell viability was reversed when treated in combination with EGCG. In contrast, the combination of EGCG with the pure ER antagonist, ICI 182, 780, showed no further reduction in cell number as only 5% of the cells were viable after 6 days of treatment. These studies may provide further understanding of the interactions among flavonoids and steroid receptors in breast cancer cells. Keywords: phytoestrogen, ER, PR, T-47D, antiestrogensHallman KAleck KQuigley MDwyer BLloyd VSzmyd MDinda SDove Medical PressarticlephytoestrogenERPRT-47Danti-estrogensNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol Volume 9, Pp 365-373 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	phytoestrogen ER PR T-47D anti-estrogens Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	phytoestrogen ER PR T-47D anti-estrogens Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hallman K Aleck K Quigley M Dwyer B Lloyd V Szmyd M Dinda S The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
description	Kelly Hallman,* Katie Aleck,* Meghan Quigley, Brigitte Dwyer, Victoria Lloyd, Monica Szmyd, Sumi Dinda Biomedical Diagnostic and Therapeutic Sciences, School of Health Sciences, Center for Biomedical Research, Oakland University, Rochester, MI, USA *These authors contributed equally to this work Abstract: It has been reported that phytoestrogen epigallocatechin gallate (EGCG) suppresses cancer cell proliferation and may have antitumor properties. In this study, we analyzed the effects of EGCG on estrogen receptor α (ERα) and progesterone receptor in hormone-dependent T-47D breast cancer cells. Western blot analysis revealed EGCG induced a concentration-dependent decrease in ERα protein levels, with a 56% reduction occurring with 60 µM EGCG when compared to controls. Downregulation of ERα protein levels was observed after 24-hour co-treatment of T-47D cells with 60 µM EGCG and 10 nM 17β-estradiol (E2). The proliferative effect of E2 on cell viability was reversed when treated in combination with EGCG. In contrast, the combination of EGCG with the pure ER antagonist, ICI 182, 780, showed no further reduction in cell number as only 5% of the cells were viable after 6 days of treatment. These studies may provide further understanding of the interactions among flavonoids and steroid receptors in breast cancer cells. Keywords: phytoestrogen, ER, PR, T-47D, antiestrogens
format	article
author	Hallman K Aleck K Quigley M Dwyer B Lloyd V Szmyd M Dinda S
author_facet	Hallman K Aleck K Quigley M Dwyer B Lloyd V Szmyd M Dinda S
author_sort	Hallman K
title	The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
title_short	The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
title_full	The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
title_fullStr	The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
title_full_unstemmed	The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
title_sort	regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/fe8db0e36a0f4dc0a73130cf806a57bf
work_keys_str_mv	AT hallmank theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT aleckk theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT quigleym theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT dwyerb theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT lloydv theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT szmydm theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT dindas theregulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT hallmank regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT aleckk regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT quigleym regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT dwyerb regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT lloydv regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT szmydm regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells AT dindas regulationofsteroidreceptorsbyepigallocatechin3gallateinbreastcancercells
_version_	1718403901805297664

The regulation of steroid receptors by epigallocatechin-3-gallate in breast cancer cells

Ejemplares similares