A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte

Abstract Sox9 is a member of the gene family of SOX transcription factors, which is highly conserved among vertebrates. It is involved in different developmental processes including gonadogenesis. In all amniote species examined thus far, Sox9 is expressed in the Sertoli cells of the male gonad, sug...

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Autores principales:	M. Penrad-Mobayed, C. Perrin, D. L’Hôte, V. Contremoulins, J.-A. Lepesant, B. Boizet-Bonhoure, F. Poulat, X. Baudin, R. A. Veitia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe8e408ee4ef459893c03808ebdab456
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spelling	oai:doaj.org-article:fe8e408ee4ef459893c03808ebdab4562021-12-02T16:08:13ZA role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte10.1038/s41598-018-25356-12045-2322https://doaj.org/article/fe8e408ee4ef459893c03808ebdab4562018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25356-1https://doaj.org/toc/2045-2322Abstract Sox9 is a member of the gene family of SOX transcription factors, which is highly conserved among vertebrates. It is involved in different developmental processes including gonadogenesis. In all amniote species examined thus far, Sox9 is expressed in the Sertoli cells of the male gonad, suggesting an evolutionarily conserved role in testis development. However, in the anamniotes, fishes and amphibians, it is also expressed in the oocyte but the significance of such an expression remains to be elucidated. Here, we have investigated the nuclear localization of the SOX9 protein in the oocyte of three amphibian species, the urodelan Pleurodeles waltl, and two anurans, Xenopus laevis and Xenopus tropicalis. We demonstrate that SOX9 is associated with ribonucleoprotein (RNP) transcripts of lampbrush chromosomes in an RNA-dependent manner. This association can be visualized by Super-resolution Structured Illumination Microscopy (SIM). Our results suggest that SOX9, known to bind DNA, also carries an additional function in the posttranscriptional processes. We also discuss the significance of the acquisition or loss of Sox9 expression in the oocyte during evolution at the transition between anamniotes and amniotes.M. Penrad-MobayedC. PerrinD. L’HôteV. ContremoulinsJ.-A. LepesantB. Boizet-BonhoureF. PoulatX. BaudinR. A. VeitiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Penrad-Mobayed C. Perrin D. L’Hôte V. Contremoulins J.-A. Lepesant B. Boizet-Bonhoure F. Poulat X. Baudin R. A. Veitia A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
description	Abstract Sox9 is a member of the gene family of SOX transcription factors, which is highly conserved among vertebrates. It is involved in different developmental processes including gonadogenesis. In all amniote species examined thus far, Sox9 is expressed in the Sertoli cells of the male gonad, suggesting an evolutionarily conserved role in testis development. However, in the anamniotes, fishes and amphibians, it is also expressed in the oocyte but the significance of such an expression remains to be elucidated. Here, we have investigated the nuclear localization of the SOX9 protein in the oocyte of three amphibian species, the urodelan Pleurodeles waltl, and two anurans, Xenopus laevis and Xenopus tropicalis. We demonstrate that SOX9 is associated with ribonucleoprotein (RNP) transcripts of lampbrush chromosomes in an RNA-dependent manner. This association can be visualized by Super-resolution Structured Illumination Microscopy (SIM). Our results suggest that SOX9, known to bind DNA, also carries an additional function in the posttranscriptional processes. We also discuss the significance of the acquisition or loss of Sox9 expression in the oocyte during evolution at the transition between anamniotes and amniotes.
format	article
author	M. Penrad-Mobayed C. Perrin D. L’Hôte V. Contremoulins J.-A. Lepesant B. Boizet-Bonhoure F. Poulat X. Baudin R. A. Veitia
author_facet	M. Penrad-Mobayed C. Perrin D. L’Hôte V. Contremoulins J.-A. Lepesant B. Boizet-Bonhoure F. Poulat X. Baudin R. A. Veitia
author_sort	M. Penrad-Mobayed
title	A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
title_short	A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
title_full	A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
title_fullStr	A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
title_full_unstemmed	A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte
title_sort	role for sox9 in post-transcriptional processes: insights from the amphibian oocyte
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fe8e408ee4ef459893c03808ebdab456
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A role for SOX9 in post-transcriptional processes: insights from the amphibian oocyte

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