Indoor Dust as a Matrix for Surveillance of COVID-19

ABSTRACT Ongoing disease surveillance is a critical tool to mitigate viral outbreaks, especially during a pandemic. Environmental monitoring has significant promise even following widespread vaccination among high-risk populations. The goal of this work is to demonstrate molecular severe acute respi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nicole Renninger, Nicholas Nastasi, Ashleigh Bope, Samuel J. Cochran, Sarah R. Haines, Neeraja Balasubrahmaniam, Katelyn Stuart, Aaron Bivins, Kyle Bibby, Natalie M. Hull, Karen C. Dannemiller
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	SARS-CoV-2 COVID-19 dust surveillance virus indoor environment Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fe9381846dd64e40bac54ad7838d8c01
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fe9381846dd64e40bac54ad7838d8c01
record_format	dspace
spelling	oai:doaj.org-article:fe9381846dd64e40bac54ad7838d8c012021-12-02T19:36:39ZIndoor Dust as a Matrix for Surveillance of COVID-1910.1128/mSystems.01350-202379-5077https://doaj.org/article/fe9381846dd64e40bac54ad7838d8c012021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01350-20https://doaj.org/toc/2379-5077ABSTRACT Ongoing disease surveillance is a critical tool to mitigate viral outbreaks, especially during a pandemic. Environmental monitoring has significant promise even following widespread vaccination among high-risk populations. The goal of this work is to demonstrate molecular severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) monitoring in bulk floor dust and related samples as a proof of concept of a noninvasive environmental surveillance methodology for coronavirus disease 2019 (COVID-19) and potentially other viral diseases. Surface swab, passive sampler, and bulk floor dust samples were collected from the rooms of individuals positive for COVID-19, and SARS-CoV-2 was measured with quantitative reverse transcription-PCR (RT-qPCR) and two digital PCR (dPCR) methods. Bulk dust samples had a geometric mean concentration of 163 copies/mg of dust and ranged from nondetects to 23,049 copies/mg of dust detected using droplet digital PCR (ddPCR). An average of 89% of bulk dust samples were positive for the virus by the detection methods compared to 55% of surface swabs and fewer on the passive sampler (19% carpet, 29% polystyrene). In bulk dust, SARS-CoV-2 was detected in 76%, 93%, and 97% of samples measured by qPCR, chip-based dPCR, and droplet dPCR, respectively. Detectable viral RNA in the bulk vacuum bags did not measurably decay over 4 weeks, despite the application of a disinfectant before room cleaning. Future monitoring efforts should further evaluate RNA persistence and heterogeneity in dust. This study did not measure virus infectivity in dust or potential transmission associated with dust. Overall, this work demonstrates that bulk floor dust is a potentially useful matrix for long-term monitoring of viral disease in high-risk populations and buildings. IMPORTANCE Environmental surveillance to assess pathogen presence within a community is proving to be a critical tool to protect public health, and it is especially relevant during the ongoing COVID-19 pandemic. Importantly, environmental surveillance tools also allow for the detection of asymptomatic disease carriers and for routine monitoring of a large number of people as has been shown for SARS-CoV-2 wastewater monitoring. However, additional monitoring techniques are needed to screen for outbreaks in high-risk settings such as congregate care facilities. Here, we demonstrate that SARS-CoV-2 can be detected in bulk floor dust collected from rooms housing infected individuals. This analysis suggests that dust may be a useful and efficient matrix for routine surveillance of viral disease.Nicole RenningerNicholas NastasiAshleigh BopeSamuel J. CochranSarah R. HainesNeeraja BalasubrahmaniamKatelyn StuartAaron BivinsKyle BibbyNatalie M. HullKaren C. DannemillerAmerican Society for MicrobiologyarticleSARS-CoV-2COVID-19dustsurveillancevirusindoor environmentMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 COVID-19 dust surveillance virus indoor environment Microbiology QR1-502
spellingShingle	SARS-CoV-2 COVID-19 dust surveillance virus indoor environment Microbiology QR1-502 Nicole Renninger Nicholas Nastasi Ashleigh Bope Samuel J. Cochran Sarah R. Haines Neeraja Balasubrahmaniam Katelyn Stuart Aaron Bivins Kyle Bibby Natalie M. Hull Karen C. Dannemiller Indoor Dust as a Matrix for Surveillance of COVID-19
description	ABSTRACT Ongoing disease surveillance is a critical tool to mitigate viral outbreaks, especially during a pandemic. Environmental monitoring has significant promise even following widespread vaccination among high-risk populations. The goal of this work is to demonstrate molecular severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) monitoring in bulk floor dust and related samples as a proof of concept of a noninvasive environmental surveillance methodology for coronavirus disease 2019 (COVID-19) and potentially other viral diseases. Surface swab, passive sampler, and bulk floor dust samples were collected from the rooms of individuals positive for COVID-19, and SARS-CoV-2 was measured with quantitative reverse transcription-PCR (RT-qPCR) and two digital PCR (dPCR) methods. Bulk dust samples had a geometric mean concentration of 163 copies/mg of dust and ranged from nondetects to 23,049 copies/mg of dust detected using droplet digital PCR (ddPCR). An average of 89% of bulk dust samples were positive for the virus by the detection methods compared to 55% of surface swabs and fewer on the passive sampler (19% carpet, 29% polystyrene). In bulk dust, SARS-CoV-2 was detected in 76%, 93%, and 97% of samples measured by qPCR, chip-based dPCR, and droplet dPCR, respectively. Detectable viral RNA in the bulk vacuum bags did not measurably decay over 4 weeks, despite the application of a disinfectant before room cleaning. Future monitoring efforts should further evaluate RNA persistence and heterogeneity in dust. This study did not measure virus infectivity in dust or potential transmission associated with dust. Overall, this work demonstrates that bulk floor dust is a potentially useful matrix for long-term monitoring of viral disease in high-risk populations and buildings. IMPORTANCE Environmental surveillance to assess pathogen presence within a community is proving to be a critical tool to protect public health, and it is especially relevant during the ongoing COVID-19 pandemic. Importantly, environmental surveillance tools also allow for the detection of asymptomatic disease carriers and for routine monitoring of a large number of people as has been shown for SARS-CoV-2 wastewater monitoring. However, additional monitoring techniques are needed to screen for outbreaks in high-risk settings such as congregate care facilities. Here, we demonstrate that SARS-CoV-2 can be detected in bulk floor dust collected from rooms housing infected individuals. This analysis suggests that dust may be a useful and efficient matrix for routine surveillance of viral disease.
format	article
author	Nicole Renninger Nicholas Nastasi Ashleigh Bope Samuel J. Cochran Sarah R. Haines Neeraja Balasubrahmaniam Katelyn Stuart Aaron Bivins Kyle Bibby Natalie M. Hull Karen C. Dannemiller
author_facet	Nicole Renninger Nicholas Nastasi Ashleigh Bope Samuel J. Cochran Sarah R. Haines Neeraja Balasubrahmaniam Katelyn Stuart Aaron Bivins Kyle Bibby Natalie M. Hull Karen C. Dannemiller
author_sort	Nicole Renninger
title	Indoor Dust as a Matrix for Surveillance of COVID-19
title_short	Indoor Dust as a Matrix for Surveillance of COVID-19
title_full	Indoor Dust as a Matrix for Surveillance of COVID-19
title_fullStr	Indoor Dust as a Matrix for Surveillance of COVID-19
title_full_unstemmed	Indoor Dust as a Matrix for Surveillance of COVID-19
title_sort	indoor dust as a matrix for surveillance of covid-19
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/fe9381846dd64e40bac54ad7838d8c01
work_keys_str_mv	AT nicolerenninger indoordustasamatrixforsurveillanceofcovid19 AT nicholasnastasi indoordustasamatrixforsurveillanceofcovid19 AT ashleighbope indoordustasamatrixforsurveillanceofcovid19 AT samueljcochran indoordustasamatrixforsurveillanceofcovid19 AT sarahrhaines indoordustasamatrixforsurveillanceofcovid19 AT neerajabalasubrahmaniam indoordustasamatrixforsurveillanceofcovid19 AT katelynstuart indoordustasamatrixforsurveillanceofcovid19 AT aaronbivins indoordustasamatrixforsurveillanceofcovid19 AT kylebibby indoordustasamatrixforsurveillanceofcovid19 AT nataliemhull indoordustasamatrixforsurveillanceofcovid19 AT karencdannemiller indoordustasamatrixforsurveillanceofcovid19
_version_	1718376360232091648

Indoor Dust as a Matrix for Surveillance of COVID-19

Ejemplares similares