Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.

Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific immunotherapy that is used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms of SCIT, we aimed to profile lung samples to explore changes in the differential proteome before and a...

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Autores principales:	Zhijuan Xie, Haidan Sun, Xiaogang Li, Wei Sun, Jia Yin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fe93a78364a941799d0eb5ae81ad93c6
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spelling	oai:doaj.org-article:fe93a78364a941799d0eb5ae81ad93c62021-12-02T20:17:11ZAlteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.1932-620310.1371/journal.pone.0258051https://doaj.org/article/fe93a78364a941799d0eb5ae81ad93c62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258051https://doaj.org/toc/1932-6203Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific immunotherapy that is used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms of SCIT, we aimed to profile lung samples to explore changes in the differential proteome before and after SCIT in mice with allergic asthma. Fresh lungs were collected from three groups of female BALB/c mice: 1) control mice, 2) birch pollen-induced allergic mice, and 3) birch pollen-induced allergic mice with SCIT. Tandem mass tag (TMT) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the lung proteome in the mice. Ingenuity pathway analysis (IPA) and Gene Ontology (GO) classification analysis were applied to identify differentially expressed proteins (DEPs) and crucial pathways. The screened DEPs were validated by immunohistochemistry analysis. A total of 317 proteins were upregulated and 184 proteins were downregulated in the asthma group compared to those of the control group. In contrast, 639 DEPs (163 upregulated and 456 downregulated proteins) were identified after SCIT in comparison with those of the asthma group. Among the 639 DEPs, 277 proteins returned to similar levels as those of the relative non-asthma condition. Bioinformatic analysis revealed that the 277 proteins played a significant role in the leukocyte extravasation signaling pathway. The leukocyte extravasation signaling pathway and related DEPs were of crucial importance in birch pollen SCIT.Zhijuan XieHaidan SunXiaogang LiWei SunJia YinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258051 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhijuan Xie Haidan Sun Xiaogang Li Wei Sun Jia Yin Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
description	Subcutaneous immunotherapy (SCIT) is a classic form of allergen-specific immunotherapy that is used to treat birch pollen induced allergic asthma. To investigate the underlying molecular mechanisms of SCIT, we aimed to profile lung samples to explore changes in the differential proteome before and after SCIT in mice with allergic asthma. Fresh lungs were collected from three groups of female BALB/c mice: 1) control mice, 2) birch pollen-induced allergic mice, and 3) birch pollen-induced allergic mice with SCIT. Tandem mass tag (TMT) labelling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the lung proteome in the mice. Ingenuity pathway analysis (IPA) and Gene Ontology (GO) classification analysis were applied to identify differentially expressed proteins (DEPs) and crucial pathways. The screened DEPs were validated by immunohistochemistry analysis. A total of 317 proteins were upregulated and 184 proteins were downregulated in the asthma group compared to those of the control group. In contrast, 639 DEPs (163 upregulated and 456 downregulated proteins) were identified after SCIT in comparison with those of the asthma group. Among the 639 DEPs, 277 proteins returned to similar levels as those of the relative non-asthma condition. Bioinformatic analysis revealed that the 277 proteins played a significant role in the leukocyte extravasation signaling pathway. The leukocyte extravasation signaling pathway and related DEPs were of crucial importance in birch pollen SCIT.
format	article
author	Zhijuan Xie Haidan Sun Xiaogang Li Wei Sun Jia Yin
author_facet	Zhijuan Xie Haidan Sun Xiaogang Li Wei Sun Jia Yin
author_sort	Zhijuan Xie
title	Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
title_short	Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
title_full	Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
title_fullStr	Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
title_full_unstemmed	Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.
title_sort	alteration of lung tissues proteins in birch pollen induced asthma mice before and after scit.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/fe93a78364a941799d0eb5ae81ad93c6
work_keys_str_mv	AT zhijuanxie alterationoflungtissuesproteinsinbirchpolleninducedasthmamicebeforeandafterscit AT haidansun alterationoflungtissuesproteinsinbirchpolleninducedasthmamicebeforeandafterscit AT xiaogangli alterationoflungtissuesproteinsinbirchpolleninducedasthmamicebeforeandafterscit AT weisun alterationoflungtissuesproteinsinbirchpolleninducedasthmamicebeforeandafterscit AT jiayin alterationoflungtissuesproteinsinbirchpolleninducedasthmamicebeforeandafterscit
_version_	1718374443780145152

Alteration of lung tissues proteins in birch pollen induced asthma mice before and after SCIT.

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