Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action

Siti Hajar Musa,1 Mahiran Basri,1 Hamid Reza Fard Masoumi,1 Norashikin Shamsudin,2 Norazlinaliza Salim1 1Department of Chemistry, Faculty of Science, 2Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia Abstract: Psoriasis is a chronic auto...

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Autores principales:	Musa SH, Basri M, Fard Masoumi HR, Shamsudin N, Salim N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Ciclosporin nanoemulsion mixture experimental design psoriasis Ostwald ripening Franz-diffusion cell transepidermal water loss Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fea8305e5e10482ca579bd1695c5b298
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spelling	oai:doaj.org-article:fea8305e5e10482ca579bd1695c5b2982021-12-02T01:11:29ZEnhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action1178-2013https://doaj.org/article/fea8305e5e10482ca579bd1695c5b2982017-03-01T00:00:00Zhttps://www.dovepress.com/enhancement-of-physicochemical-properties-of-nanocolloidal-carrier-loa-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Siti Hajar Musa,1 Mahiran Basri,1 Hamid Reza Fard Masoumi,1 Norashikin Shamsudin,2 Norazlinaliza Salim1 1Department of Chemistry, Faculty of Science, 2Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia Abstract: Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient compliance. Introducing a nanoemulsion for transporting cyclosporine as an anti-inflammatory drug to an itchy site of skin disease would enhance the effectiveness of topical treatment for psoriasis. The addition of nutmeg and virgin coconut-oil mixture, with their unique properties, could improve cyclosporine loading and solubility. A high-shear homogenizer was used in formulating a cyclosporine-loaded nanoemulsion. A D-optimal mixture experimental design was used in the optimization of nanoemulsion compositions, in order to understand the relationships behind the effect of independent variables (oil, surfactant, xanthan gum, and water content) on physicochemical response (particle size and polydispersity index) and rheological response (viscosity and k-value). Investigation of these variables suggests two optimized formulations with specific oil (15% and 20%), surfactant (15%), xanthan gum (0.75%), and water content (67.55% and 62.55%), which possessed intended responses and good stability against separation over 3 months’ storage at different temperatures. Optimized nanoemulsions of pH 4.5 were further studied with all types of stability analysis: physical stability, coalescence-rate analysis, Ostwald ripening, and freeze–thaw cycles. In vitro release proved the efficacy of nanosize emulsions in carrying cyclosporine across rat skin and a synthetic membrane that best fit the Korsmeyer–Peppas kinetic model. In vivo skin analysis towards healthy volunteers showed a significant improvement in the stratum corneum in skin hydration. Keywords: cyclosporine, nanoemulsion, mixture experimental design, psoriasis, Ostwald ripening, Franz diffusion cell, transepidermal water lossMusa SHBasri MFard Masoumi HRShamsudin NSalim NDove Medical PressarticleCiclosporinnanoemulsionmixture experimental designpsoriasisOstwald ripeningFranz-diffusion celltransepidermal water lossMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2427-2441 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ciclosporin nanoemulsion mixture experimental design psoriasis Ostwald ripening Franz-diffusion cell transepidermal water loss Medicine (General) R5-920
spellingShingle	Ciclosporin nanoemulsion mixture experimental design psoriasis Ostwald ripening Franz-diffusion cell transepidermal water loss Medicine (General) R5-920 Musa SH Basri M Fard Masoumi HR Shamsudin N Salim N Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
description	Siti Hajar Musa,1 Mahiran Basri,1 Hamid Reza Fard Masoumi,1 Norashikin Shamsudin,2 Norazlinaliza Salim1 1Department of Chemistry, Faculty of Science, 2Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia Abstract: Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient compliance. Introducing a nanoemulsion for transporting cyclosporine as an anti-inflammatory drug to an itchy site of skin disease would enhance the effectiveness of topical treatment for psoriasis. The addition of nutmeg and virgin coconut-oil mixture, with their unique properties, could improve cyclosporine loading and solubility. A high-shear homogenizer was used in formulating a cyclosporine-loaded nanoemulsion. A D-optimal mixture experimental design was used in the optimization of nanoemulsion compositions, in order to understand the relationships behind the effect of independent variables (oil, surfactant, xanthan gum, and water content) on physicochemical response (particle size and polydispersity index) and rheological response (viscosity and k-value). Investigation of these variables suggests two optimized formulations with specific oil (15% and 20%), surfactant (15%), xanthan gum (0.75%), and water content (67.55% and 62.55%), which possessed intended responses and good stability against separation over 3 months’ storage at different temperatures. Optimized nanoemulsions of pH 4.5 were further studied with all types of stability analysis: physical stability, coalescence-rate analysis, Ostwald ripening, and freeze–thaw cycles. In vitro release proved the efficacy of nanosize emulsions in carrying cyclosporine across rat skin and a synthetic membrane that best fit the Korsmeyer–Peppas kinetic model. In vivo skin analysis towards healthy volunteers showed a significant improvement in the stratum corneum in skin hydration. Keywords: cyclosporine, nanoemulsion, mixture experimental design, psoriasis, Ostwald ripening, Franz diffusion cell, transepidermal water loss
format	article
author	Musa SH Basri M Fard Masoumi HR Shamsudin N Salim N
author_facet	Musa SH Basri M Fard Masoumi HR Shamsudin N Salim N
author_sort	Musa SH
title	Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
title_short	Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
title_full	Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
title_fullStr	Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
title_full_unstemmed	Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
title_sort	enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/fea8305e5e10482ca579bd1695c5b298
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Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action

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