Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk

Abstract Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary prope...

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Autores principales:	Meguya Ryu, Armandas Balčytis, Xuewen Wang, Jitraporn Vongsvivut, Yuta Hikima, Jingliang Li, Mark J. Tobin, Saulius Juodkazis, Junko Morikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fea9b1baee6949489aa2b9208f90699b
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spelling	oai:doaj.org-article:fea9b1baee6949489aa2b9208f90699b2021-12-02T16:06:31ZOrientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk10.1038/s41598-017-07502-32045-2322https://doaj.org/article/fea9b1baee6949489aa2b9208f90699b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07502-3https://doaj.org/toc/2045-2322Abstract Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary properties of silk and its amorphous-crystalline composition has to be accessed by a direct measurement from a single silk fiber. Here, we show orientation mapping of the internal silk fiber structure via polarisation-dependent IR absorbance at high spatial resolution of 4.2 μm and 1.9 μm in a hyper-spectral IR imaging by attenuated total reflection using synchrotron radiation in the spectral fingerprint region around 6 μm wavelength. Free-standing longitudinal micro-slices of silk fibers, thinner than the fiber cross section, were prepared by microtome for the four polarization method to directly measure the orientational sensitivity of absorbance in the molecular fingerprint spectral window of the amide bands of β-sheet polypeptides of silk. Microtomed lateral slices of silk fibers, which may avoid possible artefacts that affect spectroscopic measurements with fibers of an elliptical cross sections were used in the study. Amorphisation of silk by ultra-short laser single-pulse exposure is demonstrated.Meguya RyuArmandas BalčytisXuewen WangJitraporn VongsvivutYuta HikimaJingliang LiMark J. TobinSaulius JuodkazisJunko MorikawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meguya Ryu Armandas Balčytis Xuewen Wang Jitraporn Vongsvivut Yuta Hikima Jingliang Li Mark J. Tobin Saulius Juodkazis Junko Morikawa Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
description	Abstract Molecular alignment underpins optical, mechanical, and thermal properties of materials, however, its direct measurement from volumes with micrometer dimensions is not accessible, especially, for structurally complex bio-materials. How the molecular alignment is linked to extraordinary properties of silk and its amorphous-crystalline composition has to be accessed by a direct measurement from a single silk fiber. Here, we show orientation mapping of the internal silk fiber structure via polarisation-dependent IR absorbance at high spatial resolution of 4.2 μm and 1.9 μm in a hyper-spectral IR imaging by attenuated total reflection using synchrotron radiation in the spectral fingerprint region around 6 μm wavelength. Free-standing longitudinal micro-slices of silk fibers, thinner than the fiber cross section, were prepared by microtome for the four polarization method to directly measure the orientational sensitivity of absorbance in the molecular fingerprint spectral window of the amide bands of β-sheet polypeptides of silk. Microtomed lateral slices of silk fibers, which may avoid possible artefacts that affect spectroscopic measurements with fibers of an elliptical cross sections were used in the study. Amorphisation of silk by ultra-short laser single-pulse exposure is demonstrated.
format	article
author	Meguya Ryu Armandas Balčytis Xuewen Wang Jitraporn Vongsvivut Yuta Hikima Jingliang Li Mark J. Tobin Saulius Juodkazis Junko Morikawa
author_facet	Meguya Ryu Armandas Balčytis Xuewen Wang Jitraporn Vongsvivut Yuta Hikima Jingliang Li Mark J. Tobin Saulius Juodkazis Junko Morikawa
author_sort	Meguya Ryu
title	Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
title_short	Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
title_full	Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
title_fullStr	Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
title_full_unstemmed	Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk
title_sort	orientational mapping augmented sub-wavelength hyper-spectral imaging of silk
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fea9b1baee6949489aa2b9208f90699b
work_keys_str_mv	AT meguyaryu orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT armandasbalcytis orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT xuewenwang orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT jitrapornvongsvivut orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT yutahikima orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT jingliangli orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT markjtobin orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT sauliusjuodkazis orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk AT junkomorikawa orientationalmappingaugmentedsubwavelengthhyperspectralimagingofsilk
_version_	1718385003326341120

Orientational Mapping Augmented Sub-Wavelength Hyper-Spectral Imaging of Silk

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