Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats
Izabela Czyzynska-Cichon,1,* Małgorzata Janik-Hazuka,2,* Joanna Szafraniec-Szczęsny,2,3 Krzysztof Jasinski,4 Władysław P Węglarz,4 Szczepan Zapotoczny,2 Stefan Chlopicki1,5 1Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Krakow, 30-348, Poland; 2Jagiellonian Unive...
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Dove Medical Press
2021
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oai:doaj.org-article:fec67b989f8542ed80e72ae9e13e8c822021-12-02T13:43:49ZLow Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats1178-2013https://doaj.org/article/fec67b989f8542ed80e72ae9e13e8c822021-02-01T00:00:00Zhttps://www.dovepress.com/low-dose-curcumin-administered-in-hyaluronic-acid-based-nanocapsules-i-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Izabela Czyzynska-Cichon,1,* Małgorzata Janik-Hazuka,2,* Joanna Szafraniec-Szczęsny,2,3 Krzysztof Jasinski,4 Władysław P Węglarz,4 Szczepan Zapotoczny,2 Stefan Chlopicki1,5 1Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Krakow, 30-348, Poland; 2Jagiellonian University, Faculty of Chemistry, Krakow, 30-387, Poland; 3Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmaceutics, Krakow, 30-688, Poland; 4Institute of Nuclear Physics Polish Academy of Sciences, Department of Magnetic Resonance Imaging, Krakow, 31-342, Poland; 5Jagiellonian University Medical College, Faculty of Medicine, Department of Pharmacology, Krakow, 31-531, Poland*These authors contributed equally to this workCorrespondence: Stefan Chlopicki; Szczepan Zapotoczny Email stefan.chlopicki@jcet.eu; zapotocz@chemia.uj.edu.plBackground: Vascular drug delivery becomes a promising direction in the development of novel therapeutic strategies in the treatment of cardiovascular pathologies, such as hypertension. However, targeted delivery of hydrophobic substances, with poor bioavailability, remains a challenge. Here, we described the hypotensive effects of a low dose of curcumin delivered to the vascular wall using hyaluronic acid-based nanocapsules.Methods: The group of hypertensive TGR(m-Ren2)27 rats, was administrated respectively with the vehicle, curcumin solution or curcumin delivered using hyaluronic acid-based nanocapsules (HyC12-Cur), for 7 days each, maintaining the wash-out period between treatments. Arterial blood pressure (systolic - SBP, diastolic – DBP) and heart rate (HR) were monitored continuously using a telemetry system (Data Science International), and Mean Arterial Pressure (MAP) was calculated from SBP and DBP.Results: In hypertensive rats, a low dose of curcumin (4.5 mg/kg) administrated in HyC12-Cur for 7 days resulted in a gradual inhibition of SBP, DBP and MAP increase without an effect on HR. At the end of HyC12-Cur – based treatment changes in SBP, DBP and MAP amounted to − 2.0± 0.8 mmHg, − 3.9± 0.7 mmHg and − 3.3± 0.7 mmHg, respectively. In contrast, the administration of a curcumin solution (4.5 mg/kg) did not result in a significant hypotensive effect and the animals constantly developed hypertension. Vascular delivery of capsules with curcumin was confirmed using newly developed fluorine-rich nanocapsules (HyFC10-PFOB) with a shell based on a HA derivative and similar size as HyC12-Cur. HyFC10-PFOB gave fluorine signals in rat aortas analyzed ex vivo with a 19F NMR technique after a single intragastric administration.Conclusion: These results suggest that nanocapsules based on hyaluronic acid, the ubiquitous glycosaminoglycan of the extracellular matrix and an integral part of endothelial glycocalyx, may represent a suitable approach to deliver hydrophobic, poorly bioavailable compounds, to the vascular wall.Keywords: nanocapsules, hyaluronic acid, 19F NMR, curcumin, hypertension, targeted vascular deliveryCzyzynska-Cichon IJanik-Hazuka MSzafraniec-Szczęsny JJasinski KWęglarz WPZapotoczny SChlopicki SDove Medical Pressarticlenanocapsuleshyaluronic acid19f nmrcurcuminhypertensiontargeted vascular deliveryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 1377-1390 (2021) |
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nanocapsules hyaluronic acid 19f nmr curcumin hypertension targeted vascular delivery Medicine (General) R5-920 |
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nanocapsules hyaluronic acid 19f nmr curcumin hypertension targeted vascular delivery Medicine (General) R5-920 Czyzynska-Cichon I Janik-Hazuka M Szafraniec-Szczęsny J Jasinski K Węglarz WP Zapotoczny S Chlopicki S Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
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Izabela Czyzynska-Cichon,1,* Małgorzata Janik-Hazuka,2,* Joanna Szafraniec-Szczęsny,2,3 Krzysztof Jasinski,4 Władysław P Węglarz,4 Szczepan Zapotoczny,2 Stefan Chlopicki1,5 1Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Krakow, 30-348, Poland; 2Jagiellonian University, Faculty of Chemistry, Krakow, 30-387, Poland; 3Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Technology and Biopharmaceutics, Krakow, 30-688, Poland; 4Institute of Nuclear Physics Polish Academy of Sciences, Department of Magnetic Resonance Imaging, Krakow, 31-342, Poland; 5Jagiellonian University Medical College, Faculty of Medicine, Department of Pharmacology, Krakow, 31-531, Poland*These authors contributed equally to this workCorrespondence: Stefan Chlopicki; Szczepan Zapotoczny Email stefan.chlopicki@jcet.eu; zapotocz@chemia.uj.edu.plBackground: Vascular drug delivery becomes a promising direction in the development of novel therapeutic strategies in the treatment of cardiovascular pathologies, such as hypertension. However, targeted delivery of hydrophobic substances, with poor bioavailability, remains a challenge. Here, we described the hypotensive effects of a low dose of curcumin delivered to the vascular wall using hyaluronic acid-based nanocapsules.Methods: The group of hypertensive TGR(m-Ren2)27 rats, was administrated respectively with the vehicle, curcumin solution or curcumin delivered using hyaluronic acid-based nanocapsules (HyC12-Cur), for 7 days each, maintaining the wash-out period between treatments. Arterial blood pressure (systolic - SBP, diastolic – DBP) and heart rate (HR) were monitored continuously using a telemetry system (Data Science International), and Mean Arterial Pressure (MAP) was calculated from SBP and DBP.Results: In hypertensive rats, a low dose of curcumin (4.5 mg/kg) administrated in HyC12-Cur for 7 days resulted in a gradual inhibition of SBP, DBP and MAP increase without an effect on HR. At the end of HyC12-Cur – based treatment changes in SBP, DBP and MAP amounted to − 2.0± 0.8 mmHg, − 3.9± 0.7 mmHg and − 3.3± 0.7 mmHg, respectively. In contrast, the administration of a curcumin solution (4.5 mg/kg) did not result in a significant hypotensive effect and the animals constantly developed hypertension. Vascular delivery of capsules with curcumin was confirmed using newly developed fluorine-rich nanocapsules (HyFC10-PFOB) with a shell based on a HA derivative and similar size as HyC12-Cur. HyFC10-PFOB gave fluorine signals in rat aortas analyzed ex vivo with a 19F NMR technique after a single intragastric administration.Conclusion: These results suggest that nanocapsules based on hyaluronic acid, the ubiquitous glycosaminoglycan of the extracellular matrix and an integral part of endothelial glycocalyx, may represent a suitable approach to deliver hydrophobic, poorly bioavailable compounds, to the vascular wall.Keywords: nanocapsules, hyaluronic acid, 19F NMR, curcumin, hypertension, targeted vascular delivery |
format |
article |
author |
Czyzynska-Cichon I Janik-Hazuka M Szafraniec-Szczęsny J Jasinski K Węglarz WP Zapotoczny S Chlopicki S |
author_facet |
Czyzynska-Cichon I Janik-Hazuka M Szafraniec-Szczęsny J Jasinski K Węglarz WP Zapotoczny S Chlopicki S |
author_sort |
Czyzynska-Cichon I |
title |
Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
title_short |
Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
title_full |
Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
title_fullStr |
Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
title_full_unstemmed |
Low Dose Curcumin Administered in Hyaluronic Acid-Based Nanocapsules Induces Hypotensive Effect in Hypertensive Rats |
title_sort |
low dose curcumin administered in hyaluronic acid-based nanocapsules induces hypotensive effect in hypertensive rats |
publisher |
Dove Medical Press |
publishDate |
2021 |
url |
https://doaj.org/article/fec67b989f8542ed80e72ae9e13e8c82 |
work_keys_str_mv |
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