Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019

To understand the biological characteristics of the reemerging pseudorabies virus (PRV) strains, a total of 392 tissue samples were collected from diseased pigs during reemerging PR outbreaks between 2012 and 2019 on farms in central China where swine had been immunized with Bartha-K61 and 51 (13. 0...

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Autores principales:	Hui-Hua Zheng, Yi-Lin Bai, Tong Xu, Lan-Lan Zheng, Xin-Sheng Li, Hong-Ying Chen, Zhen-Ya Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	pseudorabies virus sequencing phylogenetic analysis reemerging virus pig disease Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/fec8fa039c2642bd9405dd0502399ec1
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spelling	oai:doaj.org-article:fec8fa039c2642bd9405dd0502399ec12021-11-16T06:53:40ZIsolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 20192297-176910.3389/fvets.2021.764982https://doaj.org/article/fec8fa039c2642bd9405dd0502399ec12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.764982/fullhttps://doaj.org/toc/2297-1769To understand the biological characteristics of the reemerging pseudorabies virus (PRV) strains, a total of 392 tissue samples were collected from diseased pigs during reemerging PR outbreaks between 2012 and 2019 on farms in central China where swine had been immunized with Bartha-K61 and 51 (13. 01%) were positive for the gE gene by PCR. Sixteen PRV strains were isolated and caused clinical symptoms and death in mice. Subsequently, gE, gC, gB, and gD complete genes were amplified from the 16 PRV isolates and sequenced. Phylogenetic analysis based on these four gene sequences shows that the 16 PRV isolates were more closely related to the Chinese PRV variants (after 2012) but genetically differed from early Chinese PRV isolates (before 2012). Sequence analysis reveals that PRV isolates exhibited amino acid insertions, substitutions, or deletions compared with early Chinese PRV isolates and European–American PRV strains. In addition, this is the first report that eight isolates (8/16) in this study harbor a unique amino acid substitution at position 280 (F to L) of the gC protein, and six isolates have an amino acid substitution at position 338 (A to V) of the gD protein compared with the Chinese PRV variants. The emulsion containing inactivated PRV NY isolate could provide complete protection against the NY isolate. This study might enrich our understanding of the evolution of reemerging PRV strains as well as pave the way for finding a model virus to develop a novel vaccine based on reemerging PRV strains.Hui-Hua ZhengYi-Lin BaiTong XuLan-Lan ZhengXin-Sheng LiHong-Ying ChenZhen-Ya WangFrontiers Media S.A.articlepseudorabies virussequencingphylogenetic analysisreemerging viruspig diseaseVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pseudorabies virus sequencing phylogenetic analysis reemerging virus pig disease Veterinary medicine SF600-1100
spellingShingle	pseudorabies virus sequencing phylogenetic analysis reemerging virus pig disease Veterinary medicine SF600-1100 Hui-Hua Zheng Yi-Lin Bai Tong Xu Lan-Lan Zheng Xin-Sheng Li Hong-Ying Chen Zhen-Ya Wang Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
description	To understand the biological characteristics of the reemerging pseudorabies virus (PRV) strains, a total of 392 tissue samples were collected from diseased pigs during reemerging PR outbreaks between 2012 and 2019 on farms in central China where swine had been immunized with Bartha-K61 and 51 (13. 01%) were positive for the gE gene by PCR. Sixteen PRV strains were isolated and caused clinical symptoms and death in mice. Subsequently, gE, gC, gB, and gD complete genes were amplified from the 16 PRV isolates and sequenced. Phylogenetic analysis based on these four gene sequences shows that the 16 PRV isolates were more closely related to the Chinese PRV variants (after 2012) but genetically differed from early Chinese PRV isolates (before 2012). Sequence analysis reveals that PRV isolates exhibited amino acid insertions, substitutions, or deletions compared with early Chinese PRV isolates and European–American PRV strains. In addition, this is the first report that eight isolates (8/16) in this study harbor a unique amino acid substitution at position 280 (F to L) of the gC protein, and six isolates have an amino acid substitution at position 338 (A to V) of the gD protein compared with the Chinese PRV variants. The emulsion containing inactivated PRV NY isolate could provide complete protection against the NY isolate. This study might enrich our understanding of the evolution of reemerging PRV strains as well as pave the way for finding a model virus to develop a novel vaccine based on reemerging PRV strains.
format	article
author	Hui-Hua Zheng Yi-Lin Bai Tong Xu Lan-Lan Zheng Xin-Sheng Li Hong-Ying Chen Zhen-Ya Wang
author_facet	Hui-Hua Zheng Yi-Lin Bai Tong Xu Lan-Lan Zheng Xin-Sheng Li Hong-Ying Chen Zhen-Ya Wang
author_sort	Hui-Hua Zheng
title	Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
title_short	Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
title_full	Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
title_fullStr	Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
title_full_unstemmed	Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019
title_sort	isolation and phylogenetic analysis of reemerging pseudorabies virus within pig populations in central china during 2012 to 2019
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fec8fa039c2642bd9405dd0502399ec1
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_version_	1718426704088662016

Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019

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