Functional role of Mst1/Mst2 in embryonic stem cell differentiation.

The Hippo pathway is an evolutionary conserved pathway that involves cell proliferation, differentiation, apoptosis and organ size regulation. Mst1 and Mst2 are central components of this pathway that are essential for embryonic development, though their role in controlling embryonic stem cells (ES...

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Autores principales:	Peng Li, Ying Chen, Kinglun Kingston Mak, Chun Kwok Wong, Chi Chiu Wang, Ping Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fed2036ea5f0413caaceeb5d6df4de90
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spelling	oai:doaj.org-article:fed2036ea5f0413caaceeb5d6df4de902021-11-18T08:48:15ZFunctional role of Mst1/Mst2 in embryonic stem cell differentiation.1932-620310.1371/journal.pone.0079867https://doaj.org/article/fed2036ea5f0413caaceeb5d6df4de902013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24224013/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The Hippo pathway is an evolutionary conserved pathway that involves cell proliferation, differentiation, apoptosis and organ size regulation. Mst1 and Mst2 are central components of this pathway that are essential for embryonic development, though their role in controlling embryonic stem cells (ES cells) has yet to be exploited. To further understand the Mst1/Mst2 function in ES cell pluripotency and differentiation, we derived Mst1/Mst2 double knockout (Mst-/-) ES cells to completely perturb Hippo signaling. We found that Mst-/- ES cells express higher level of Nanog than wild type ES cells and show differentiation resistance after LIF withdrawal. They also proliferate faster than wild type ES cells. Although Mst-/- ES cells can form embryoid bodies (EBs), their differentiation into tissues of three germ layers is distorted. Intriguingly, Mst-/- ES cells are unable to form teratoma. Mst-/- ES cells can differentiate into mesoderm lineage, but further differentiation to cardiac lineage cells is significantly affected. Microarray analysis revealed that ligands of non-canonical Wnt signaling, which is critical for cardiac progenitor specification, are significantly repressed in Mst-/- EBs. Taken together our results showed that Mst1/Mst2 are required for proper cardiac lineage cell development and teratoma formation.Peng LiYing ChenKinglun Kingston MakChun Kwok WongChi Chiu WangPing YuanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e79867 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Li Ying Chen Kinglun Kingston Mak Chun Kwok Wong Chi Chiu Wang Ping Yuan Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
description	The Hippo pathway is an evolutionary conserved pathway that involves cell proliferation, differentiation, apoptosis and organ size regulation. Mst1 and Mst2 are central components of this pathway that are essential for embryonic development, though their role in controlling embryonic stem cells (ES cells) has yet to be exploited. To further understand the Mst1/Mst2 function in ES cell pluripotency and differentiation, we derived Mst1/Mst2 double knockout (Mst-/-) ES cells to completely perturb Hippo signaling. We found that Mst-/- ES cells express higher level of Nanog than wild type ES cells and show differentiation resistance after LIF withdrawal. They also proliferate faster than wild type ES cells. Although Mst-/- ES cells can form embryoid bodies (EBs), their differentiation into tissues of three germ layers is distorted. Intriguingly, Mst-/- ES cells are unable to form teratoma. Mst-/- ES cells can differentiate into mesoderm lineage, but further differentiation to cardiac lineage cells is significantly affected. Microarray analysis revealed that ligands of non-canonical Wnt signaling, which is critical for cardiac progenitor specification, are significantly repressed in Mst-/- EBs. Taken together our results showed that Mst1/Mst2 are required for proper cardiac lineage cell development and teratoma formation.
format	article
author	Peng Li Ying Chen Kinglun Kingston Mak Chun Kwok Wong Chi Chiu Wang Ping Yuan
author_facet	Peng Li Ying Chen Kinglun Kingston Mak Chun Kwok Wong Chi Chiu Wang Ping Yuan
author_sort	Peng Li
title	Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
title_short	Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
title_full	Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
title_fullStr	Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
title_full_unstemmed	Functional role of Mst1/Mst2 in embryonic stem cell differentiation.
title_sort	functional role of mst1/mst2 in embryonic stem cell differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fed2036ea5f0413caaceeb5d6df4de90
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_version_	1718421301546188800

Functional role of Mst1/Mst2 in embryonic stem cell differentiation.

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