Repression of Akt3 gene transcription by the tumor suppressor RIZ1

Abstract RIZ1 has been studied as a tumor suppressor and may play a role in metabolic diseases related to the Western style diet, such as cancer and obesity. The Akt pathway is known to play a role in both cancer and obesity, and a link between Akt and RIZ1 has also been found. To better understand...

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Autores principales:	Qingnan Liu, Xiaotian Qu, Xiaolei Xie, Pei He, Shi Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fede120718e1409bae95cd626a0302ff
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spelling	oai:doaj.org-article:fede120718e1409bae95cd626a0302ff2021-12-02T15:08:23ZRepression of Akt3 gene transcription by the tumor suppressor RIZ110.1038/s41598-018-19943-52045-2322https://doaj.org/article/fede120718e1409bae95cd626a0302ff2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19943-5https://doaj.org/toc/2045-2322Abstract RIZ1 has been studied as a tumor suppressor and may play a role in metabolic diseases related to the Western style diet, such as cancer and obesity. The Akt pathway is known to play a role in both cancer and obesity, and a link between Akt and RIZ1 has also been found. To better understand the role of RIZ1 in obesity and cancer, we investigated how RIZ1 regulates the expression of Akt3. We found that overexpression of RIZ1 in HEK293 cells reduced the expression of Akt3 protein. Luciferase reporter activity of Akt3 gene promoter was significantly reduced in cells co-transfected with RIZ1. Recombinant proteins of RIZ1 was able to bind the Akt3 promoter in vitro, and chromatin immunoprecipitation assay also demonstrated the ability of RIZ1 binding to the Akt3 promoter in vivo. Overexpression of RIZ1 increased H3K9 methylation on the Akt3 promoter. These results identify Akt3 as a target of RIZ1 regulation and expand our understanding of the Akt pathway in cancer and obesity.Qingnan LiuXiaotian QuXiaolei XiePei HeShi HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qingnan Liu Xiaotian Qu Xiaolei Xie Pei He Shi Huang Repression of Akt3 gene transcription by the tumor suppressor RIZ1
description	Abstract RIZ1 has been studied as a tumor suppressor and may play a role in metabolic diseases related to the Western style diet, such as cancer and obesity. The Akt pathway is known to play a role in both cancer and obesity, and a link between Akt and RIZ1 has also been found. To better understand the role of RIZ1 in obesity and cancer, we investigated how RIZ1 regulates the expression of Akt3. We found that overexpression of RIZ1 in HEK293 cells reduced the expression of Akt3 protein. Luciferase reporter activity of Akt3 gene promoter was significantly reduced in cells co-transfected with RIZ1. Recombinant proteins of RIZ1 was able to bind the Akt3 promoter in vitro, and chromatin immunoprecipitation assay also demonstrated the ability of RIZ1 binding to the Akt3 promoter in vivo. Overexpression of RIZ1 increased H3K9 methylation on the Akt3 promoter. These results identify Akt3 as a target of RIZ1 regulation and expand our understanding of the Akt pathway in cancer and obesity.
format	article
author	Qingnan Liu Xiaotian Qu Xiaolei Xie Pei He Shi Huang
author_facet	Qingnan Liu Xiaotian Qu Xiaolei Xie Pei He Shi Huang
author_sort	Qingnan Liu
title	Repression of Akt3 gene transcription by the tumor suppressor RIZ1
title_short	Repression of Akt3 gene transcription by the tumor suppressor RIZ1
title_full	Repression of Akt3 gene transcription by the tumor suppressor RIZ1
title_fullStr	Repression of Akt3 gene transcription by the tumor suppressor RIZ1
title_full_unstemmed	Repression of Akt3 gene transcription by the tumor suppressor RIZ1
title_sort	repression of akt3 gene transcription by the tumor suppressor riz1
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fede120718e1409bae95cd626a0302ff
work_keys_str_mv	AT qingnanliu repressionofakt3genetranscriptionbythetumorsuppressorriz1 AT xiaotianqu repressionofakt3genetranscriptionbythetumorsuppressorriz1 AT xiaoleixie repressionofakt3genetranscriptionbythetumorsuppressorriz1 AT peihe repressionofakt3genetranscriptionbythetumorsuppressorriz1 AT shihuang repressionofakt3genetranscriptionbythetumorsuppressorriz1
_version_	1718388166095798272

Repression of Akt3 gene transcription by the tumor suppressor RIZ1

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