Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro

Genistein exhibits anti-diabetic and anti-lipogenic effects in mammals, however, there are rare reports on the effects of genistein on glucose and lipid metabolism in fish. This study explored the regulation role of genistein on glucose and lipid metabolism of common carp. After oral gavage with gen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liping Yang, Wenlei Zhang, Shaoyang Zhi, Mingyu Liu, Mengjuan Zhao, Chaobin Qin, Xiao Yan, Junchang Feng, Guoxing Nie
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Genistein Cyprinus carpio. L Absorption Glucose metabolism Lipid metabolism Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/fedea53551964c5da5b79c331b264a15
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fedea53551964c5da5b79c331b264a15
record_format	dspace
spelling	oai:doaj.org-article:fedea53551964c5da5b79c331b264a152021-11-12T04:41:26ZEffects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro2352-513410.1016/j.aqrep.2021.100930https://doaj.org/article/fedea53551964c5da5b79c331b264a152022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003471https://doaj.org/toc/2352-5134Genistein exhibits anti-diabetic and anti-lipogenic effects in mammals, however, there are rare reports on the effects of genistein on glucose and lipid metabolism in fish. This study explored the regulation role of genistein on glucose and lipid metabolism of common carp. After oral gavage with genistein (20 mg/kg body weight), the content of genistein in serum was the highest at 4 h and rapidly returned to basal values at 48 h. The blood glucose (GLU) concentrations were decreased at 24 h after gavage with genistein. The total cholesterol (TC) level was significantly down-regulated by genistein except for 24 h (P < 0.01). On the contrary, serum triglycerides (TG), LDL-C, and HDL-C were significantly up-regulated after treatment (P < 0.05). The mRNA level of the genes related to gluconeogenesis, glycogen synthesis, and lipolysis was significantly inhibited after treatment with genistein. Meanwhile, genistein administration has up-regulated the level of lipid synthesis-related genes. To compare the effects of genistein with estradiol, the primary hepatocytes were incubated with different concentrations of genistein (1, 10, or 100 µM) and 17β-estradiol (E2, 1 µM) respectively. Similar to the in vivo results, the lipid synthesis-related genes levels were increased and lipolysis genes were decreased after treatment with a high dose of genistein (100 µM). On the contrary, the genes involved in lipid synthesis were down-regulated, while lipolysis genes were up-regulated at the low and medium doses of genistein (1 and 10 µM). Moreover, low and medium doses of genistein enhanced the expression of gluconeogenesis-related genes. There is a certain similar effect of estrogen and genistein in vitro, it is speculated that genistein may play an estrogen-like role in regulating glucose and lipid metabolism. The effect of genistein on fish glucose and lipid metabolism may be affected by the concentration level, treatment time, or the methods.Liping YangWenlei ZhangShaoyang ZhiMingyu LiuMengjuan ZhaoChaobin QinXiao YanJunchang FengGuoxing NieElsevierarticleGenisteinCyprinus carpio. LAbsorptionGlucose metabolismLipid metabolismAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100930- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genistein Cyprinus carpio. L Absorption Glucose metabolism Lipid metabolism Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Genistein Cyprinus carpio. L Absorption Glucose metabolism Lipid metabolism Aquaculture. Fisheries. Angling SH1-691 Liping Yang Wenlei Zhang Shaoyang Zhi Mingyu Liu Mengjuan Zhao Chaobin Qin Xiao Yan Junchang Feng Guoxing Nie Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
description	Genistein exhibits anti-diabetic and anti-lipogenic effects in mammals, however, there are rare reports on the effects of genistein on glucose and lipid metabolism in fish. This study explored the regulation role of genistein on glucose and lipid metabolism of common carp. After oral gavage with genistein (20 mg/kg body weight), the content of genistein in serum was the highest at 4 h and rapidly returned to basal values at 48 h. The blood glucose (GLU) concentrations were decreased at 24 h after gavage with genistein. The total cholesterol (TC) level was significantly down-regulated by genistein except for 24 h (P < 0.01). On the contrary, serum triglycerides (TG), LDL-C, and HDL-C were significantly up-regulated after treatment (P < 0.05). The mRNA level of the genes related to gluconeogenesis, glycogen synthesis, and lipolysis was significantly inhibited after treatment with genistein. Meanwhile, genistein administration has up-regulated the level of lipid synthesis-related genes. To compare the effects of genistein with estradiol, the primary hepatocytes were incubated with different concentrations of genistein (1, 10, or 100 µM) and 17β-estradiol (E2, 1 µM) respectively. Similar to the in vivo results, the lipid synthesis-related genes levels were increased and lipolysis genes were decreased after treatment with a high dose of genistein (100 µM). On the contrary, the genes involved in lipid synthesis were down-regulated, while lipolysis genes were up-regulated at the low and medium doses of genistein (1 and 10 µM). Moreover, low and medium doses of genistein enhanced the expression of gluconeogenesis-related genes. There is a certain similar effect of estrogen and genistein in vitro, it is speculated that genistein may play an estrogen-like role in regulating glucose and lipid metabolism. The effect of genistein on fish glucose and lipid metabolism may be affected by the concentration level, treatment time, or the methods.
format	article
author	Liping Yang Wenlei Zhang Shaoyang Zhi Mingyu Liu Mengjuan Zhao Chaobin Qin Xiao Yan Junchang Feng Guoxing Nie
author_facet	Liping Yang Wenlei Zhang Shaoyang Zhi Mingyu Liu Mengjuan Zhao Chaobin Qin Xiao Yan Junchang Feng Guoxing Nie
author_sort	Liping Yang
title	Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
title_short	Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
title_full	Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
title_fullStr	Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
title_full_unstemmed	Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro
title_sort	effects of genistein on glucose and lipid metabolism of common carp (cyprinus carpio. l) in vivo and in vitro
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/fedea53551964c5da5b79c331b264a15
work_keys_str_mv	AT lipingyang effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT wenleizhang effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT shaoyangzhi effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT mingyuliu effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT mengjuanzhao effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT chaobinqin effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT xiaoyan effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT junchangfeng effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro AT guoxingnie effectsofgenisteinonglucoseandlipidmetabolismofcommoncarpcyprinuscarpiolinvivoandinvitro
_version_	1718431267796549632

Effects of genistein on glucose and lipid metabolism of common carp (Cyprinus carpio. L) in vivo and in vitro

Ejemplares similares