Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles

Mingfu Gong,1 Hua Yang,1,2 Song Zhang,1 Yan Yang,1 Dong Zhang,1 Zhaohui Li,3 Liguang Zou1 1Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China; 2Department of Radiology, Chongqing Traditional Chinese Medicine Hospital, Chongq...

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Autores principales:	Gong M, Yang H, Zhang S, Yang Y, Zhang D, Li Z, Zou L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	CL1555 CL-PEG-MnFe2O4 TVECs T1/T2 dual-modality MRI Tumor angiogenesis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fee0c87d31674411996217766c8f2171
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spelling	oai:doaj.org-article:fee0c87d31674411996217766c8f21712021-12-02T05:04:29ZTargeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles1178-2013https://doaj.org/article/fee0c87d31674411996217766c8f21712016-08-01T00:00:00Zhttps://www.dovepress.com/targeting-t1-and-t2-dual-modality-enhanced-magnetic-resonance-imaging--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Mingfu Gong,1 Hua Yang,1,2 Song Zhang,1 Yan Yang,1 Dong Zhang,1 Zhaohui Li,3 Liguang Zou1 1Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China; 2Department of Radiology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, People’s Republic of China; 3Geosciences Department, University of Wisconsin-Parkside, Kenosha, WI, USA Abstract: Tumor angiogenesis plays very important roles for tumorigenesis, tumor development, metastasis, and prognosis. Targeting T1/T2 dual modality magnetic resonance (MR) imaging of the tumor vascular endothelial cells (TVECs) with MR molecular probes can greatly improve diagnostic sensitivity and specificity, as well as helping to make an early diagnosis of tumor at the preclinical stage. In this study, a new T1 and T2 dual modality nanoprobe was successfully fabricated. The prepared nanoprobe comprise peptides CL 1555, poly(ε-caprolactone)-block-poly(ethylene glycol) amphiphilic copolymer shell, and dozens of manganese ferrite (MnFe2O4) nanoparticle core. The results showed that the hydrophobic MnFe2O4 nanoparticles were of uniform spheroidal appearance and narrow size distribution. Due to the self-assembled nanomicelles structure, the prepared probes were of high relaxivity of 281.7 mM-1 s-1, which was much higher than that of MnFe2O4 nanoparticles (67.5 mM-1 s-1). After being grafted with the targeted CD105 peptide CL 1555, the nanomicelles can combine TVECs specifically and make the labeled TVECs dark in T2-weighted MR imaging. With the passage on, the Mn2+ ions were released from MnFe2O4 and the size decreased gradually, making the signal intensity of the second and third passage of labeled TVECs increased in T1-weighted MR imaging. Our results demonstrate that CL-poly(ethylene glycol)-MnFe2O4 can conjugate TVECs and induce dark and bright contrast in MR imaging, and act as a novel molecular probe for T1- and T2-enhanced MR imaging of tumor angiogenesis. Keywords: CL 1555, CL-PEG-MnFe2O4, TVECs, CD105, tumor angiogenesisGong MYang HZhang SYang YZhang DLi ZZou LDove Medical PressarticleCL1555CL-PEG-MnFe2O4TVECsT1/T2 dual-modality MRITumor angiogenesisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 4051-4063 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	CL1555 CL-PEG-MnFe2O4 TVECs T1/T2 dual-modality MRI Tumor angiogenesis Medicine (General) R5-920
spellingShingle	CL1555 CL-PEG-MnFe2O4 TVECs T1/T2 dual-modality MRI Tumor angiogenesis Medicine (General) R5-920 Gong M Yang H Zhang S Yang Y Zhang D Li Z Zou L Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
description	Mingfu Gong,1 Hua Yang,1,2 Song Zhang,1 Yan Yang,1 Dong Zhang,1 Zhaohui Li,3 Liguang Zou1 1Department of Radiology, Xinqiao Hospital, Third Military Medical University, Chongqing, People’s Republic of China; 2Department of Radiology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, People’s Republic of China; 3Geosciences Department, University of Wisconsin-Parkside, Kenosha, WI, USA Abstract: Tumor angiogenesis plays very important roles for tumorigenesis, tumor development, metastasis, and prognosis. Targeting T1/T2 dual modality magnetic resonance (MR) imaging of the tumor vascular endothelial cells (TVECs) with MR molecular probes can greatly improve diagnostic sensitivity and specificity, as well as helping to make an early diagnosis of tumor at the preclinical stage. In this study, a new T1 and T2 dual modality nanoprobe was successfully fabricated. The prepared nanoprobe comprise peptides CL 1555, poly(ε-caprolactone)-block-poly(ethylene glycol) amphiphilic copolymer shell, and dozens of manganese ferrite (MnFe2O4) nanoparticle core. The results showed that the hydrophobic MnFe2O4 nanoparticles were of uniform spheroidal appearance and narrow size distribution. Due to the self-assembled nanomicelles structure, the prepared probes were of high relaxivity of 281.7 mM-1 s-1, which was much higher than that of MnFe2O4 nanoparticles (67.5 mM-1 s-1). After being grafted with the targeted CD105 peptide CL 1555, the nanomicelles can combine TVECs specifically and make the labeled TVECs dark in T2-weighted MR imaging. With the passage on, the Mn2+ ions were released from MnFe2O4 and the size decreased gradually, making the signal intensity of the second and third passage of labeled TVECs increased in T1-weighted MR imaging. Our results demonstrate that CL-poly(ethylene glycol)-MnFe2O4 can conjugate TVECs and induce dark and bright contrast in MR imaging, and act as a novel molecular probe for T1- and T2-enhanced MR imaging of tumor angiogenesis. Keywords: CL 1555, CL-PEG-MnFe2O4, TVECs, CD105, tumor angiogenesis
format	article
author	Gong M Yang H Zhang S Yang Y Zhang D Li Z Zou L
author_facet	Gong M Yang H Zhang S Yang Y Zhang D Li Z Zou L
author_sort	Gong M
title	Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
title_short	Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
title_full	Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
title_fullStr	Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
title_full_unstemmed	Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
title_sort	targeting t1 and t2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/fee0c87d31674411996217766c8f2171
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Targeting T1 and T2 dual modality enhanced magnetic resonance imaging of tumor vascular endothelial cells based on peptides-conjugated manganese ferrite nanomicelles

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