The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.

<h4>Background</h4>Detecting a new effective and hypotoxic anticancer drug is an emerging new strategy for cancer chemotherapy. Doxycycline (DC) is a kind of antibiotics but also inhibits tumorigenesis.<h4>Methods</h4>MTT and cell invasion assay, flow cytometry, western-blot...

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Autores principales:	Wei Wu, Li-Hua Yu, Bei Ma, Ming-Juan Xu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fee10e1eb0e14229a6adecb88761f271
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spelling	oai:doaj.org-article:fee10e1eb0e14229a6adecb88761f2712021-11-18T08:29:41ZThe inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.1932-620310.1371/journal.pone.0089841https://doaj.org/article/fee10e1eb0e14229a6adecb88761f2712014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24598933/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Detecting a new effective and hypotoxic anticancer drug is an emerging new strategy for cancer chemotherapy. Doxycycline (DC) is a kind of antibiotics but also inhibits tumorigenesis.<h4>Methods</h4>MTT and cell invasion assay, flow cytometry, western-blot analysis and nude mice were used to investigate the effects and underlying mechanisms of doxycycline on epithelial ovarian cancer cells.<h4>Results</h4>Doxycycline inhibited the proliferation and invasion of SKOV3 and SKOV3/DDP; induced moderate apoptosis of SKOV3/DDP. CXCR4 expression at both mRNA and protein levels was downregulated in both cell lines when treated with doxycycline. Akt and ERK1/2 were involved in doxycycline effect on cell proliferation of SKOV3 but not of SKOV3/DDP. Akt and EKR1/2 phosphorylation were activated by SDF-1α, which was then inhibited by doxycycline in SKOV3. Pro-caspase-3 expression was significantly higher in SKOV3 than that in SKOV3/DDP which was upregulated when treated with doxycycline. In vivo, doxycycline inhibited peritoneal tumor xenograft and decreased malignant ascites.<h4>Conclusion</h4>Doxycycline not only has an inhibitory effect on ovarian cancer, but also can increase sensitivity to cisplatin. SDF-1α/CXCR4-regulated Akt and ERK 1/2 activations are probably involved in the antitumor effect of doxycycline on SKOV3 cells, while upregulation of pro-caspase-3 may be the main mechanism involved in SKOV3/DDP cells.Wei WuLi-Hua YuBei MaMing-Juan XuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e89841 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Wu Li-Hua Yu Bei Ma Ming-Juan Xu The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
description	<h4>Background</h4>Detecting a new effective and hypotoxic anticancer drug is an emerging new strategy for cancer chemotherapy. Doxycycline (DC) is a kind of antibiotics but also inhibits tumorigenesis.<h4>Methods</h4>MTT and cell invasion assay, flow cytometry, western-blot analysis and nude mice were used to investigate the effects and underlying mechanisms of doxycycline on epithelial ovarian cancer cells.<h4>Results</h4>Doxycycline inhibited the proliferation and invasion of SKOV3 and SKOV3/DDP; induced moderate apoptosis of SKOV3/DDP. CXCR4 expression at both mRNA and protein levels was downregulated in both cell lines when treated with doxycycline. Akt and ERK1/2 were involved in doxycycline effect on cell proliferation of SKOV3 but not of SKOV3/DDP. Akt and EKR1/2 phosphorylation were activated by SDF-1α, which was then inhibited by doxycycline in SKOV3. Pro-caspase-3 expression was significantly higher in SKOV3 than that in SKOV3/DDP which was upregulated when treated with doxycycline. In vivo, doxycycline inhibited peritoneal tumor xenograft and decreased malignant ascites.<h4>Conclusion</h4>Doxycycline not only has an inhibitory effect on ovarian cancer, but also can increase sensitivity to cisplatin. SDF-1α/CXCR4-regulated Akt and ERK 1/2 activations are probably involved in the antitumor effect of doxycycline on SKOV3 cells, while upregulation of pro-caspase-3 may be the main mechanism involved in SKOV3/DDP cells.
format	article
author	Wei Wu Li-Hua Yu Bei Ma Ming-Juan Xu
author_facet	Wei Wu Li-Hua Yu Bei Ma Ming-Juan Xu
author_sort	Wei Wu
title	The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
title_short	The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
title_full	The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
title_fullStr	The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
title_full_unstemmed	The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
title_sort	inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fee10e1eb0e14229a6adecb88761f271
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The inhibitory effect of doxycycline on cisplatin-sensitive and -resistant epithelial ovarian cancer.

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