Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides

Abstract Antibodies have different chemical properties capable of targeting a diverse nature of antigens. Traditionally, immune antibody libraries are perceived to be disease-specific with a skewed repertoire. The complexity during the generation of a combinatorial antibody library allows for a skew...

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Autores principales:	Anizah Rahumatullah, Dinesh Balachandra, Rahmah Noordin, Zamrina Baharudeen, Yee Ying Lim, Yee Siew Choong, Theam Soon Lim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fee29d8c22f84d63ba6aa14747bdfd8c
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spelling	oai:doaj.org-article:fee29d8c22f84d63ba6aa14747bdfd8c2021-12-02T13:57:37ZBroad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides10.1038/s41598-021-82125-32045-2322https://doaj.org/article/fee29d8c22f84d63ba6aa14747bdfd8c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82125-3https://doaj.org/toc/2045-2322Abstract Antibodies have different chemical properties capable of targeting a diverse nature of antigens. Traditionally, immune antibody libraries are perceived to be disease-specific with a skewed repertoire. The complexity during the generation of a combinatorial antibody library allows for a skewed but diverse repertoire to be generated. Strongyloides stercoralis is a parasite that causes strongyloidiasis, a potentially life-threatening disease with a complex diagnosis that impedes effective control and treatment of the disease. This study describes the isolation of monoclonal antibodies against S. stercoralis NIE recombinant protein using an immune antibody phage display library derived from lymphatic filaria-infected individuals. The isolated antibody clones showed both lambda and kappa light chains gene usage, with diverse amino acid distributions. Structural analysis showed that electropositivity and the interface area could determine the binding affinity of the clones with NIE. The successful identification of S. stercoralis antibodies from the filarial immune library highlights the breadth of antibody gene diversification in an immune antibody library that can be applied for closely related infections.Anizah RahumatullahDinesh BalachandraRahmah NoordinZamrina BaharudeenYee Ying LimYee Siew ChoongTheam Soon LimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anizah Rahumatullah Dinesh Balachandra Rahmah Noordin Zamrina Baharudeen Yee Ying Lim Yee Siew Choong Theam Soon Lim Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
description	Abstract Antibodies have different chemical properties capable of targeting a diverse nature of antigens. Traditionally, immune antibody libraries are perceived to be disease-specific with a skewed repertoire. The complexity during the generation of a combinatorial antibody library allows for a skewed but diverse repertoire to be generated. Strongyloides stercoralis is a parasite that causes strongyloidiasis, a potentially life-threatening disease with a complex diagnosis that impedes effective control and treatment of the disease. This study describes the isolation of monoclonal antibodies against S. stercoralis NIE recombinant protein using an immune antibody phage display library derived from lymphatic filaria-infected individuals. The isolated antibody clones showed both lambda and kappa light chains gene usage, with diverse amino acid distributions. Structural analysis showed that electropositivity and the interface area could determine the binding affinity of the clones with NIE. The successful identification of S. stercoralis antibodies from the filarial immune library highlights the breadth of antibody gene diversification in an immune antibody library that can be applied for closely related infections.
format	article
author	Anizah Rahumatullah Dinesh Balachandra Rahmah Noordin Zamrina Baharudeen Yee Ying Lim Yee Siew Choong Theam Soon Lim
author_facet	Anizah Rahumatullah Dinesh Balachandra Rahmah Noordin Zamrina Baharudeen Yee Ying Lim Yee Siew Choong Theam Soon Lim
author_sort	Anizah Rahumatullah
title	Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
title_short	Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
title_full	Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
title_fullStr	Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
title_full_unstemmed	Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides
title_sort	broad specificity of immune helminth scfv library to identify monoclonal antibodies targeting strongyloides
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fee29d8c22f84d63ba6aa14747bdfd8c
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Broad specificity of immune helminth scFv library to identify monoclonal antibodies targeting Strongyloides

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