Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues
Tissue clearing has revolutionised histology, but limited penetration of antibodies and stains into thick tissue segments is still a bottleneck. Here, the authors characterise optically cleared tissue as an electrolyte gel and apply this knowledge to stain the entirety of thick tissue samples.
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fef62ea755aa42a98c3b75bb70274b5d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:fef62ea755aa42a98c3b75bb70274b5d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:fef62ea755aa42a98c3b75bb70274b5d2021-12-02T14:41:15ZVersatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues10.1038/s41467-020-15906-52041-1723https://doaj.org/article/fef62ea755aa42a98c3b75bb70274b5d2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15906-5https://doaj.org/toc/2041-1723Tissue clearing has revolutionised histology, but limited penetration of antibodies and stains into thick tissue segments is still a bottleneck. Here, the authors characterise optically cleared tissue as an electrolyte gel and apply this knowledge to stain the entirety of thick tissue samples.Etsuo A. SusakiChika ShimizuAkihiro KunoKazuki TainakaXiang LiKengo NishiKen MorishimaHiroaki OnoKoji L. OdeYuki SaekiKazunari MiyamichiKaoru IsaChihiro YokoyamaHiroki KitauraMasako IkemuraTetsuo UshikuYoshihiro ShimizuTakashi SaitoTakaomi C. SaidoMasashi FukayamaHirotaka OnoeKazushige TouharaTadashi IsaAkiyoshi KakitaMitsuhiro ShibayamaHiroki R. UedaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-22 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Etsuo A. Susaki Chika Shimizu Akihiro Kuno Kazuki Tainaka Xiang Li Kengo Nishi Ken Morishima Hiroaki Ono Koji L. Ode Yuki Saeki Kazunari Miyamichi Kaoru Isa Chihiro Yokoyama Hiroki Kitaura Masako Ikemura Tetsuo Ushiku Yoshihiro Shimizu Takashi Saito Takaomi C. Saido Masashi Fukayama Hirotaka Onoe Kazushige Touhara Tadashi Isa Akiyoshi Kakita Mitsuhiro Shibayama Hiroki R. Ueda Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| description |
Tissue clearing has revolutionised histology, but limited penetration of antibodies and stains into thick tissue segments is still a bottleneck. Here, the authors characterise optically cleared tissue as an electrolyte gel and apply this knowledge to stain the entirety of thick tissue samples. |
| format |
article |
| author |
Etsuo A. Susaki Chika Shimizu Akihiro Kuno Kazuki Tainaka Xiang Li Kengo Nishi Ken Morishima Hiroaki Ono Koji L. Ode Yuki Saeki Kazunari Miyamichi Kaoru Isa Chihiro Yokoyama Hiroki Kitaura Masako Ikemura Tetsuo Ushiku Yoshihiro Shimizu Takashi Saito Takaomi C. Saido Masashi Fukayama Hirotaka Onoe Kazushige Touhara Tadashi Isa Akiyoshi Kakita Mitsuhiro Shibayama Hiroki R. Ueda |
| author_facet |
Etsuo A. Susaki Chika Shimizu Akihiro Kuno Kazuki Tainaka Xiang Li Kengo Nishi Ken Morishima Hiroaki Ono Koji L. Ode Yuki Saeki Kazunari Miyamichi Kaoru Isa Chihiro Yokoyama Hiroki Kitaura Masako Ikemura Tetsuo Ushiku Yoshihiro Shimizu Takashi Saito Takaomi C. Saido Masashi Fukayama Hirotaka Onoe Kazushige Touhara Tadashi Isa Akiyoshi Kakita Mitsuhiro Shibayama Hiroki R. Ueda |
| author_sort |
Etsuo A. Susaki |
| title |
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| title_short |
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| title_full |
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| title_fullStr |
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| title_full_unstemmed |
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| title_sort |
versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/fef62ea755aa42a98c3b75bb70274b5d |
| work_keys_str_mv |
AT etsuoasusaki versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT chikashimizu versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT akihirokuno versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kazukitainaka versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT xiangli versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kengonishi versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kenmorishima versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT hiroakiono versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kojilode versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT yukisaeki versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kazunarimiyamichi versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kaoruisa versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT chihiroyokoyama versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT hirokikitaura versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT masakoikemura versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT tetsuoushiku versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT yoshihiroshimizu versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT takashisaito versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT takaomicsaido versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT masashifukayama versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT hirotakaonoe versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT kazushigetouhara versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT tadashiisa versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT akiyoshikakita versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT mitsuhiroshibayama versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues AT hirokirueda versatilewholeorganbodystainingandimagingbasedonelectrolytegelpropertiesofbiologicaltissues |
| _version_ |
1718389969755570176 |