First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection

The widespread use of chemical control agents and pesticides for plant-pathogen control has caused many human health and environmental issues. Plant extracts and biocontrol agents have robust antimicrobial activity against different plant pathogens. However, their antiviral activities are still bein...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahmed Abdelkhalek, Abdulaziz A. Al-Askar, Maha M. Alsubaie, Said I. Behiry
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Paronychia argentea</i> plant extract TMV tomato antioxidant enzymes gene expression Botany QK1-989
Acceso en línea:	https://doaj.org/article/fef8a2d9094d41d49e6d76ea831b28a4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fef8a2d9094d41d49e6d76ea831b28a4
record_format	dspace
spelling	oai:doaj.org-article:fef8a2d9094d41d49e6d76ea831b28a42021-11-25T18:46:34ZFirst Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection10.3390/plants101124352223-7747https://doaj.org/article/fef8a2d9094d41d49e6d76ea831b28a42021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2435https://doaj.org/toc/2223-7747The widespread use of chemical control agents and pesticides for plant-pathogen control has caused many human health and environmental issues. Plant extracts and biocontrol agents have robust antimicrobial activity against different plant pathogens. However, their antiviral activities are still being investigated. In the present study, the methanol extract of <i>Paronychia argentea</i> was characterized and evaluated for its protective activity against the tobacco mosaic virus (TMV) infection in tomato plants under greenhouse conditions at 21 days post-inoculation. The results showed that the foliar application of <i>P. argentea</i> extract (10 µg/mL) enhanced tomato plant growth, resulting in significant increases in shoot and root parameters and total chlorophyll contents. Moreover, a significant reduction in TMV accumulation level in <i>P. argentea</i>-treated plants of 77.88% compared to non-treated plants was reported. Furthermore, induction of systemic resistance with significant elevation in production of antioxidant enzymes (PPO, CAT, and SOD) and transcriptional levels of the pathogenesis-related proteins (PR-1 and PR-7) and polyphenolic genes (CHS and HQT) were also observed. Out of 16 detected compounds, HPLC analysis revealed that the most abundant polyphenolic compounds found in <i>P. argentea</i> extract were gallic acid (5.36 µg/mL), kaempferol (7.39 µg/mL), quercetin (7.44 µg/mL), ellagic acid (7.89 µg/mL), myricetin (8.36 µg/mL), and ferulic acid (8.69 µg/mL). The findings suggest that the use of <i>P. argentea</i> extract as an effective and safe source for the production of bioactive compounds may offer a solution for a promising approach for the management of plant viral infections. To the best of our knowledge, this is the first report of the protective activity of <i>P. argentea</i> extract against plant viral diseases.Ahmed AbdelkhalekAbdulaziz A. Al-AskarMaha M. AlsubaieSaid I. BehiryMDPI AGarticle<i>Paronychia argentea</i>plant extractTMVtomatoantioxidant enzymesgene expressionBotanyQK1-989ENPlants, Vol 10, Iss 2435, p 2435 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Paronychia argentea</i> plant extract TMV tomato antioxidant enzymes gene expression Botany QK1-989
spellingShingle	<i>Paronychia argentea</i> plant extract TMV tomato antioxidant enzymes gene expression Botany QK1-989 Ahmed Abdelkhalek Abdulaziz A. Al-Askar Maha M. Alsubaie Said I. Behiry First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
description	The widespread use of chemical control agents and pesticides for plant-pathogen control has caused many human health and environmental issues. Plant extracts and biocontrol agents have robust antimicrobial activity against different plant pathogens. However, their antiviral activities are still being investigated. In the present study, the methanol extract of <i>Paronychia argentea</i> was characterized and evaluated for its protective activity against the tobacco mosaic virus (TMV) infection in tomato plants under greenhouse conditions at 21 days post-inoculation. The results showed that the foliar application of <i>P. argentea</i> extract (10 µg/mL) enhanced tomato plant growth, resulting in significant increases in shoot and root parameters and total chlorophyll contents. Moreover, a significant reduction in TMV accumulation level in <i>P. argentea</i>-treated plants of 77.88% compared to non-treated plants was reported. Furthermore, induction of systemic resistance with significant elevation in production of antioxidant enzymes (PPO, CAT, and SOD) and transcriptional levels of the pathogenesis-related proteins (PR-1 and PR-7) and polyphenolic genes (CHS and HQT) were also observed. Out of 16 detected compounds, HPLC analysis revealed that the most abundant polyphenolic compounds found in <i>P. argentea</i> extract were gallic acid (5.36 µg/mL), kaempferol (7.39 µg/mL), quercetin (7.44 µg/mL), ellagic acid (7.89 µg/mL), myricetin (8.36 µg/mL), and ferulic acid (8.69 µg/mL). The findings suggest that the use of <i>P. argentea</i> extract as an effective and safe source for the production of bioactive compounds may offer a solution for a promising approach for the management of plant viral infections. To the best of our knowledge, this is the first report of the protective activity of <i>P. argentea</i> extract against plant viral diseases.
format	article
author	Ahmed Abdelkhalek Abdulaziz A. Al-Askar Maha M. Alsubaie Said I. Behiry
author_facet	Ahmed Abdelkhalek Abdulaziz A. Al-Askar Maha M. Alsubaie Said I. Behiry
author_sort	Ahmed Abdelkhalek
title	First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
title_short	First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
title_full	First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
title_fullStr	First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
title_full_unstemmed	First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection
title_sort	first report of protective activity of <i>paronychia argentea</i> extract against tobacco mosaic virus infection
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fef8a2d9094d41d49e6d76ea831b28a4
work_keys_str_mv	AT ahmedabdelkhalek firstreportofprotectiveactivityofiparonychiaargenteaiextractagainsttobaccomosaicvirusinfection AT abdulazizaalaskar firstreportofprotectiveactivityofiparonychiaargenteaiextractagainsttobaccomosaicvirusinfection AT mahamalsubaie firstreportofprotectiveactivityofiparonychiaargenteaiextractagainsttobaccomosaicvirusinfection AT saidibehiry firstreportofprotectiveactivityofiparonychiaargenteaiextractagainsttobaccomosaicvirusinfection
_version_	1718410751337562112

First Report of Protective Activity of <i>Paronychia argentea</i> Extract against Tobacco Mosaic Virus Infection

Ejemplares similares