NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas

Abstract The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatos...

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Autores principales:	Ping Tang, Hao Dang, Jie Huang, Tao Xu, Ping Yuan, Jun Hu, Jian-feng Sheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	P47phox Knockout Thyroid Cancer Cells Extracellular Acidification Rate (ECAR) NOX4 Knockdown Papillary Thyroid Cancer Cell Line Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fefb3b60bdcf4c568ab5e80878a550a8
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spelling	oai:doaj.org-article:fefb3b60bdcf4c568ab5e80878a550a82021-12-02T15:07:59ZNADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas10.1038/s41598-018-34154-82045-2322https://doaj.org/article/fefb3b60bdcf4c568ab5e80878a550a82018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34154-8https://doaj.org/toc/2045-2322Abstract The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroid cancer cells were used to study the cell growth by knocking down the expression of NOX4 and knocking out its functional partner p22phox/CYBA. As a result, the increasement of mitochondrial ROS(mROS) was abolished due to both knockdown of NOX4 and p22phox knockout in hypoxia, which destabilized HIF1α decreasing glycolysis and retarded cell growth. These data suggests that Nox4 is potent oncotarget due to its role in regulating glycolysis through mROS-HIF1α pathway, thereby mediating proliferation in thyroid carcinomas.Ping TangHao DangJie HuangTao XuPing YuanJun HuJian-feng ShengNature PortfolioarticleP47phox KnockoutThyroid Cancer CellsExtracellular Acidification Rate (ECAR)NOX4 KnockdownPapillary Thyroid Cancer Cell LineMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	P47phox Knockout Thyroid Cancer Cells Extracellular Acidification Rate (ECAR) NOX4 Knockdown Papillary Thyroid Cancer Cell Line Medicine R Science Q
spellingShingle	P47phox Knockout Thyroid Cancer Cells Extracellular Acidification Rate (ECAR) NOX4 Knockdown Papillary Thyroid Cancer Cell Line Medicine R Science Q Ping Tang Hao Dang Jie Huang Tao Xu Ping Yuan Jun Hu Jian-feng Sheng NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
description	Abstract The function of the NAD(P)H oxidases (NOXs) family member NOX4 is to generate reactive oxygen species (ROS), however, the molecular function of NOX4 has not been fully studied and waiting to be clarified. To elucidate the function of endogenous Nox4 in human thyroid carcinomas, papillomatosis thyroid cancer cells were used to study the cell growth by knocking down the expression of NOX4 and knocking out its functional partner p22phox/CYBA. As a result, the increasement of mitochondrial ROS(mROS) was abolished due to both knockdown of NOX4 and p22phox knockout in hypoxia, which destabilized HIF1α decreasing glycolysis and retarded cell growth. These data suggests that Nox4 is potent oncotarget due to its role in regulating glycolysis through mROS-HIF1α pathway, thereby mediating proliferation in thyroid carcinomas.
format	article
author	Ping Tang Hao Dang Jie Huang Tao Xu Ping Yuan Jun Hu Jian-feng Sheng
author_facet	Ping Tang Hao Dang Jie Huang Tao Xu Ping Yuan Jun Hu Jian-feng Sheng
author_sort	Ping Tang
title	NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_short	NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_full	NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_fullStr	NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_full_unstemmed	NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas
title_sort	nadph oxidase nox4 is a glycolytic regulator through mros-hif1α axis in thyroid carcinomas
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fefb3b60bdcf4c568ab5e80878a550a8
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NADPH oxidase NOX4 is a glycolytic regulator through mROS-HIF1α axis in thyroid carcinomas

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