The production of fibroblast growth factor 23 is controlled by TGF-β2

Abstract Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in cell growth, differentiation, apoptosis, and inflammation. It stimulates store-operated calcium entry (SOCE) through the calcium release-activated calcium (CRAC) channel Orai1/Stim1 in endometri...

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Autores principales:	Martina Feger, Philipp Hase, Bingbing Zhang, Frank Hirche, Philipp Glosse, Florian Lang, Michael Föller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fefce797720346d5b0acfe927ed1297b
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spelling	oai:doaj.org-article:fefce797720346d5b0acfe927ed1297b2021-12-02T16:06:08ZThe production of fibroblast growth factor 23 is controlled by TGF-β210.1038/s41598-017-05226-y2045-2322https://doaj.org/article/fefce797720346d5b0acfe927ed1297b2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05226-yhttps://doaj.org/toc/2045-2322Abstract Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in cell growth, differentiation, apoptosis, and inflammation. It stimulates store-operated calcium entry (SOCE) through the calcium release-activated calcium (CRAC) channel Orai1/Stim1 in endometrial Ishikawa cells. Bone cells generate fibroblast growth factor (FGF) 23, which inhibits renal phosphate reabsorption and 1,25(OH)2D3 formation in concert with its co-receptor Klotho. Moreover, Klotho and FGF23 counteract aging and age-related clinical conditions. FGF23 production is dependent on Orai1-mediated SOCE and inflammation. Here, we explored a putative role of TGF-β2 in FGF23 synthesis. To this end, UMR106 osteoblast-like cells were cultured, Fgf23 transcript levels determined by qRT-PCR, FGF23 protein measured by ELISA, and SOCE analyzed by fluorescence optics. UMR106 cells expressed TGF-β receptors 1 and 2. TGF-β2 enhanced SOCE and potently stimulated the production of FGF23, an effect significantly attenuated by SB431542, an inhibitor of the transforming growth factor-β (TGF-β) type I receptor activin receptor-like kinases ALK5, ALK4, and ALK7. Furthermore, the TGF-β2 effect on FGF23 production was blunted by SOCE inhibitor 2-APB. We conclude that TGF-β2 induces FGF23 production, an effect involving up-regulation of SOCE.Martina FegerPhilipp HaseBingbing ZhangFrank HirchePhilipp GlosseFlorian LangMichael FöllerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martina Feger Philipp Hase Bingbing Zhang Frank Hirche Philipp Glosse Florian Lang Michael Föller The production of fibroblast growth factor 23 is controlled by TGF-β2
description	Abstract Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in cell growth, differentiation, apoptosis, and inflammation. It stimulates store-operated calcium entry (SOCE) through the calcium release-activated calcium (CRAC) channel Orai1/Stim1 in endometrial Ishikawa cells. Bone cells generate fibroblast growth factor (FGF) 23, which inhibits renal phosphate reabsorption and 1,25(OH)2D3 formation in concert with its co-receptor Klotho. Moreover, Klotho and FGF23 counteract aging and age-related clinical conditions. FGF23 production is dependent on Orai1-mediated SOCE and inflammation. Here, we explored a putative role of TGF-β2 in FGF23 synthesis. To this end, UMR106 osteoblast-like cells were cultured, Fgf23 transcript levels determined by qRT-PCR, FGF23 protein measured by ELISA, and SOCE analyzed by fluorescence optics. UMR106 cells expressed TGF-β receptors 1 and 2. TGF-β2 enhanced SOCE and potently stimulated the production of FGF23, an effect significantly attenuated by SB431542, an inhibitor of the transforming growth factor-β (TGF-β) type I receptor activin receptor-like kinases ALK5, ALK4, and ALK7. Furthermore, the TGF-β2 effect on FGF23 production was blunted by SOCE inhibitor 2-APB. We conclude that TGF-β2 induces FGF23 production, an effect involving up-regulation of SOCE.
format	article
author	Martina Feger Philipp Hase Bingbing Zhang Frank Hirche Philipp Glosse Florian Lang Michael Föller
author_facet	Martina Feger Philipp Hase Bingbing Zhang Frank Hirche Philipp Glosse Florian Lang Michael Föller
author_sort	Martina Feger
title	The production of fibroblast growth factor 23 is controlled by TGF-β2
title_short	The production of fibroblast growth factor 23 is controlled by TGF-β2
title_full	The production of fibroblast growth factor 23 is controlled by TGF-β2
title_fullStr	The production of fibroblast growth factor 23 is controlled by TGF-β2
title_full_unstemmed	The production of fibroblast growth factor 23 is controlled by TGF-β2
title_sort	production of fibroblast growth factor 23 is controlled by tgf-β2
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fefce797720346d5b0acfe927ed1297b
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The production of fibroblast growth factor 23 is controlled by TGF-β2

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