Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.

Green tea extract (GTE) ingested by rats exerted anti-oxidative activities in various ocular tissues as shown in our previous studies. The present work investigated anti-inflammatory effects of GTE on endotoxin-induced uveitis (EIU). EIU was generated in adult rats by a footpad injection of 1 mg/kg...

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Autores principales:	Yong Jie Qin, Kai On Chu, Yolanda Wong Ying Yip, Wai Ying Li, Ya Ping Yang, Kwok Ping Chan, Jia Lin Ren, Sun On Chan, Chi Pui Pang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fefda8ef31d2456c9cbb3d2645864988
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spelling	oai:doaj.org-article:fefda8ef31d2456c9cbb3d26458649882021-11-25T06:05:53ZGreen tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.1932-620310.1371/journal.pone.0103995https://doaj.org/article/fefda8ef31d2456c9cbb3d26458649882014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25093862/?tool=EBIhttps://doaj.org/toc/1932-6203Green tea extract (GTE) ingested by rats exerted anti-oxidative activities in various ocular tissues as shown in our previous studies. The present work investigated anti-inflammatory effects of GTE on endotoxin-induced uveitis (EIU). EIU was generated in adult rats by a footpad injection of 1 mg/kg lipopolysaccharide (LPS). Oral administration of GTE (550 mg/kg) was given one, two or four times after LPS injection. Twenty-four hours later, LPS produced severe hyperemia and edema in the iris. Immunocytochemical examinations showed an accumulation of infiltrating cells in the aqueous humor that were immunopositive for cluster of differentiation 43 (CD43) and CD68, markers for leucocytes and macrophages, respectively. Analyses of the aqueous humor showed an increase in pro-inflammatory mediators including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). GTE treatments improved the clinical manifestations and reduced infiltrating cells and protein exudation in the aqueous humor, which were not observed under half dose of GTE (275 mg/kg). The number of CD68 positive macrophages residing in the iris and ciliary was also reduced. GTE suppressed production of TNF-α, IL-6 and MCP-1 in the aqueous humor, which was associated with a down-regulation of LPS receptor complex subunits, Toll-like receptor 4 (TLR-4) and CD14, and suppression of nuclear factor-kappa Bp65 (NF-κBp65) in the iris and ciliary body. Our findings show that GTE is a potent anti-inflammatory agent against the inflammation of EIU, and suggest a potential use in treatment of acute uveitis.Yong Jie QinKai On ChuYolanda Wong Ying YipWai Ying LiYa Ping YangKwok Ping ChanJia Lin RenSun On ChanChi Pui PangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103995 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong Jie Qin Kai On Chu Yolanda Wong Ying Yip Wai Ying Li Ya Ping Yang Kwok Ping Chan Jia Lin Ren Sun On Chan Chi Pui Pang Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
description	Green tea extract (GTE) ingested by rats exerted anti-oxidative activities in various ocular tissues as shown in our previous studies. The present work investigated anti-inflammatory effects of GTE on endotoxin-induced uveitis (EIU). EIU was generated in adult rats by a footpad injection of 1 mg/kg lipopolysaccharide (LPS). Oral administration of GTE (550 mg/kg) was given one, two or four times after LPS injection. Twenty-four hours later, LPS produced severe hyperemia and edema in the iris. Immunocytochemical examinations showed an accumulation of infiltrating cells in the aqueous humor that were immunopositive for cluster of differentiation 43 (CD43) and CD68, markers for leucocytes and macrophages, respectively. Analyses of the aqueous humor showed an increase in pro-inflammatory mediators including tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1). GTE treatments improved the clinical manifestations and reduced infiltrating cells and protein exudation in the aqueous humor, which were not observed under half dose of GTE (275 mg/kg). The number of CD68 positive macrophages residing in the iris and ciliary was also reduced. GTE suppressed production of TNF-α, IL-6 and MCP-1 in the aqueous humor, which was associated with a down-regulation of LPS receptor complex subunits, Toll-like receptor 4 (TLR-4) and CD14, and suppression of nuclear factor-kappa Bp65 (NF-κBp65) in the iris and ciliary body. Our findings show that GTE is a potent anti-inflammatory agent against the inflammation of EIU, and suggest a potential use in treatment of acute uveitis.
format	article
author	Yong Jie Qin Kai On Chu Yolanda Wong Ying Yip Wai Ying Li Ya Ping Yang Kwok Ping Chan Jia Lin Ren Sun On Chan Chi Pui Pang
author_facet	Yong Jie Qin Kai On Chu Yolanda Wong Ying Yip Wai Ying Li Ya Ping Yang Kwok Ping Chan Jia Lin Ren Sun On Chan Chi Pui Pang
author_sort	Yong Jie Qin
title	Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
title_short	Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
title_full	Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
title_fullStr	Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
title_full_unstemmed	Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
title_sort	green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fefda8ef31d2456c9cbb3d2645864988
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_version_	1718414201130582016

Green tea extract treatment alleviates ocular inflammation in a rat model of endotoxin-induced uveitis.

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